ADVANCES In DIELECTROPHORESIS ON-A Chip DOI
Ciprian Iliescu, Florina Silvia Iliescu

Annals of the Academy of Romanian Scientists Series on Science and Technology of Information, Journal Year: 2024, Volume and Issue: 17(2), P. 52 - 67

Published: Dec. 31, 2024

Liquid biopsy has emerged as a transformative tool in oncology, offering non-invasive alternative to traditional tissue biopsies. One approach utilizes circulating tumour cells (CTCs) from body fluids such blood, urine, and saliva provide insights into genetics dynamics. A method for isolating CTCs uses the dielectrophoresis phenomenon microfluidic platforms. The present work reviews advances on chip. First, we fundamental theory of dielectrophoresis, followed by main operating principles. We further discuss some separation techniques using dielectrophoresis. finally challenges concluding remarks. Translating DEP technology lab-scale industrial applications remains challenging due complexities creating large-scale, non-uniform electric fields.

Language: Английский

Genomic synergy in food traceability: Enhancing global food safety and security DOI
Jacob Tizhe Liberty

Ecological Genetics and Genomics, Journal Year: 2025, Volume and Issue: 34, P. 100324 - 100324

Published: Jan. 5, 2025

Language: Английский

Citations

1
Perspective Chapter: Molecular Diagnostics in Viral Outbreak Surveillance DOI
Jennifer Giandhari, Amsha Viraragavan, Michelle Gordon

et al.

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Understanding and adopting various methods for monitoring viral outbreaks is required pathogen surveillance. Accurate diagnosis can play a significant role in the safe effective treatment prescribed. Africa remains burdened with host of infectious diseases, which challenges healthcare systems proper management diseases. Surveillance are implemented some parts that have infrastructure funding to perform routine testing screening. However, not all this continent equipped necessary tools support. With travel, tourism goods exchange, diseases spread across borders rapidly, posing threat global health, emphasising unified need efforts prevent, detect, act on disease through improved access vaccinations treatments. Effective response depends several elements, including laboratory capacity, skilled health staff, surveillance detect limit illnesses rapidly. Traditional molecular such as genotyping polymerase chain reaction (PCR)-based detection now being complemented like next-generation sequencing clustered regularly interspaced short palindromic repeats (CRISPR). This book chapter aims summarise current broadly describes issue climate change its impact outbreaks.

Language: Английский

Citations

0
Advancing gut microbiome insights: Graphene quantum dot nanobiosensors for microbial metabolite detection DOI
Soheil Sadr,

Shakiba Nazemian,

Shiva Dianaty

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162954 - 162954

Published: April 1, 2025

Citations

0
CRISPR revolution: Unleashing precision pathogen detection to safeguard public health and food safety DOI
Jacob Tizhe Liberty, Sabri Bromage,

Endurance Peter

et al.

Methods, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Au–Pt Coating Improved Catalytic Stability of Au@AuPt Nanoparticles for Pressure-Based Point-of-Care Detection of Escherichia coli O157:H7 DOI
Dan Liu,

Xingbo Yu,

Congying Li

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(27), P. 34632 - 34640

Published: June 25, 2024

Point-of-care testing (POCT) technologies facilitate onsite detection of pathogens in minutes to hours. Among various POCT approaches, pressure-based sensors that utilize gas-generating reactions, particularly those catalyzed by nanozymes (e.g., platinum nanoparticles, PtNPs, or platinum-coated gold and Au@PtNPs) have been shown provide rapid sensitive capabilities. The current study introduces Au-Pt alloy-coated nanoparticles (Au@AuPtNPs), an innovative nanozyme with enhanced catalytic activity relatively high stability. For pathogen detection, Au@AuPtNPs are modified H1 H2 hairpin DNAs can be triggered undergo a hybridization chain reaction (HCR) leads their aggregation upon recognition initiator strand (Ini) H1-/H2-complementary aptamers tethered magnetic beads (MBs). Pathogen binding the aptamer exposes Ini, which then binds initiates HCR, resulting Au@AuPtNP on MBs. These aggregates exhibit strong catalysis O2 from H2O2 substrate, is measured pressure meter, enabling Escherichia coli (E. coli) O157:H7 at concentrations as low 3 CFU/mL specificity. Additionally, E. could also detected simulated water tea samples. This method eliminates need for costly, labor- training-intensive instruments, supporting its further validation deployment rapid-response application bacterial contaminants.

Language: Английский

Citations

3
Advancing microbiota therapeutics: the role of synthetic biology in engineering microbial communities for precision medicine DOI Creative Commons
Asiya Nazir,

Fathima Hasnain Nadeem Hussain,

Afsheen Raza

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 4, 2024

Over recent years, studies on microbiota research and synthetic biology have explored novel approaches microbial manipulation for therapeutic purposes. However, fragmented information is available this aspect with key insights scattered across various disciplines such as molecular biology, genetics, bioengineering, medicine. This review aims to the transformative potential of in advancing microbiome therapies, significant implications healthcare, agriculture, environmental sustainability. By merging computer science, engineering, allows precise design modification biological systems via cutting edge technologies like CRISPR/Cas9 gene editing, metabolic oligonucleotide synthesis, thus paving way targeted treatments personalized probiotics engineered microorganisms. The will also highlight vital role gut disorders caused by its dysbiosis suggesting microbiota-based therapies innovations biosensors real-time health monitoring, non-invasive diagnostic tools, automated bio foundries better outcomes. Moreover, challenges including genetic stability, safety, robust regulatory frameworks be discussed understand importance ongoing ensure safe effective interventions.

Language: Английский

Citations

3
Opportunities and challenges of point of care testing paradigms in the post-COVID era DOI Open Access
Barbara Van Der Pol

Expert Review of Molecular Diagnostics, Journal Year: 2024, Volume and Issue: 24(3), P. 135 - 137

Published: March 3, 2024

Language: Английский

Citations

2
Specific detection of gut pathogens for one-pot chip based on RPA-CRISPR/Cas12a DOI
Na Ren,

Boren Sui,

Chunhong Liu

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1318, P. 342886 - 342886

Published: June 22, 2024

Language: Английский

Citations

2
Validation and evaluation of the performance of QIAstat-Dx gastrointestinal panel in pooled stool samples DOI
Xinbin Chen,

Huina Huang,

Su Ying

et al.

Acta Tropica, Journal Year: 2024, Volume and Issue: 261, P. 107514 - 107514

Published: Dec. 26, 2024

Language: Английский

Citations

0
ADVANCES In DIELECTROPHORESIS ON-A Chip DOI
Ciprian Iliescu, Florina Silvia Iliescu

Annals of the Academy of Romanian Scientists Series on Science and Technology of Information, Journal Year: 2024, Volume and Issue: 17(2), P. 52 - 67

Published: Dec. 31, 2024

Liquid biopsy has emerged as a transformative tool in oncology, offering non-invasive alternative to traditional tissue biopsies. One approach utilizes circulating tumour cells (CTCs) from body fluids such blood, urine, and saliva provide insights into genetics dynamics. A method for isolating CTCs uses the dielectrophoresis phenomenon microfluidic platforms. The present work reviews advances on chip. First, we fundamental theory of dielectrophoresis, followed by main operating principles. We further discuss some separation techniques using dielectrophoresis. finally challenges concluding remarks. Translating DEP technology lab-scale industrial applications remains challenging due complexities creating large-scale, non-uniform electric fields.

Language: Английский

Citations

0