The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity Through the cAMP/Rac1 Pathway DOI Open Access
Tingting Gao,

Ruiyuan Gu,

Heng Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11110 - 11110

Published: Oct. 16, 2024

Contrast-induced acute kidney injury (CIAKI) is a common complication with limited treatments. Intermedin (IMD), peptide belonging to the calcitonin gene-related family, promotes vasodilation and endothelial stability, but its role in mitigating CIAKI remains unexplored. This study investigates protective effects of IMD CIAKI, focusing on mechanisms, particularly cAMP/Rac1 signaling pathway. Human umbilical vein cells (HUVECs) were treated iohexol simulate vitro. The assessed using CCK8 assay, flow cytometry, ELISA, Western blotting. A rat model was utilized evaluate renal peritubular capillary cell function through histopathology, immunohistochemistry, immunofluorescence, blotting, transmission electron microscopy. In vitro, significantly enhanced HUVEC viability mitigated iohexol-induced toxicity by preserving intercellular adhesion junctions activating pathway, Rac1 inhibition attenuating these effects. vivo, caused severe damage junctions, impairing function. treatment markedly improved function, an effect negated inhibition. protects against integrity alleviating from contrast media. These findings suggest that has therapeutic potential highlight pathway as promising target for preventing contrast-induced at-risk patients, ultimately improving clinical outcomes.

Language: Английский

Advanced visible surgical scaffold to enhance urogynaecological health DOI

Chaya Tiktin,

Rumbidzai Zizhou, Elena Pirogova

et al.

MRS Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Language: Английский

Citations

1
Iodinated contrast media (ICM)-induced thyroid dysfunction: a review of potential mechanisms and clinical management DOI Creative Commons
Yue Hu, Xia Zhong, Dan Peng

et al.

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 29, 2025

Language: Английский

Citations

0
The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity through the cAMP/Rac1 Pathway DOI Open Access
Tingting Gao,

Ruiyuan Gu,

Heng Wang

et al.

Published: Aug. 27, 2024

Background: Contrast-induced acute kidney injury (CIAKI) is a common complication in hospital-acquired renal conditions, yet its pathogenesis remains unclear and effective treatments are limited. Intermedin (IMD), peptide related to the calcitonin gene, exhibits vasodilatory endothelial barrier-stabilizing effects via receptor-like receptor (CLR). However, potential of IMD mitigating CIAKI has not been thoroughly investigated. Aims: This study aimed delineate protective role through both vitro vivo models, with particular focus on elucidating underlying mechanisms, specifically cAMP/Rac1 signaling pathway. Methods: Human umbilical vein cells (HUVECs) were treated iohexol simulate vitro. The evaluated using CCK8 assay, flow cytometry, ELISA, western blotting. A rat model was developed assess peritubular capillary cell function range techniques, including histopathology, immunohistochemistry, immunofluorescence, blotting, transmission electron microscopy. Results: In vitro, significantly enhanced HUVEC viability mitigated iohexol-induced toxicity by preserving intercellular adhesion junctions activating attenuated Rac1 inhibition. vivo, caused considerable damage junctions, leading impaired function. treatment markedly improved function, an effect that abolished Conclusion: protects against pathway, thereby integrity induced contrast media. These findings underscore therapeutic emphasize critical pathway protection.

Language: Английский

Citations

1
The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity Through the cAMP/Rac1 Pathway DOI Open Access
Tingting Gao,

Ruiyuan Gu,

Heng Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11110 - 11110

Published: Oct. 16, 2024

Contrast-induced acute kidney injury (CIAKI) is a common complication with limited treatments. Intermedin (IMD), peptide belonging to the calcitonin gene-related family, promotes vasodilation and endothelial stability, but its role in mitigating CIAKI remains unexplored. This study investigates protective effects of IMD CIAKI, focusing on mechanisms, particularly cAMP/Rac1 signaling pathway. Human umbilical vein cells (HUVECs) were treated iohexol simulate vitro. The assessed using CCK8 assay, flow cytometry, ELISA, Western blotting. A rat model was utilized evaluate renal peritubular capillary cell function through histopathology, immunohistochemistry, immunofluorescence, blotting, transmission electron microscopy. In vitro, significantly enhanced HUVEC viability mitigated iohexol-induced toxicity by preserving intercellular adhesion junctions activating pathway, Rac1 inhibition attenuating these effects. vivo, caused severe damage junctions, impairing function. treatment markedly improved function, an effect negated inhibition. protects against integrity alleviating from contrast media. These findings suggest that has therapeutic potential highlight pathway as promising target for preventing contrast-induced at-risk patients, ultimately improving clinical outcomes.

Language: Английский

Citations

0