Computational studies on the interaction of SARS-CoV-2 Omicron SGp RBD with human receptor ACE2, limonin and glycyrrhizic acid

Computers in Biology and Medicine, Journal Year: 2022, Volume and Issue: 144, P. 105367 - 105367

Published: March 1, 2022

Language: Английский

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SARS-CoV-2 vs. human gametes, embryos and cryopreservation

Systems Biology in Reproductive Medicine, Journal Year: 2021, Volume and Issue: 67(4), P. 260 - 269

Published: June 1, 2021

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, is an unprecedented global situation, and all countries have adopted their own measurements to mitigate spread of virus in first as well subsequent waves infection. All measures, especially wave were combination with recommendations provided professional scientific organizations. Similar measures applied specific procedures, such management infertility, including vitro fertilization-embryo transfer (IVF-ET) treatments. Although there no clear evidence yet that may exert negative effects on IVF outcome, at early stages, several clinical reports indicate impact male fertility through receptors presented somatic cells testis used order gain entry respective cells. Nevertheless, it not unreasonable suspect affect sperm function oocyte performance directly or indirectly other signaling pathways. Despite good practice laboratory techniques, culture media also be contaminated during equilibration when airborne virus's particles can contaminate from already infected embryology area staff. Furthermore, although evidence, liquid nitrogen could a route infection for gametes embryos has been production transportation. Therefore, cryopreservation must virus-free. This communication aims provide some aspects possible how procedures.

Language: Английский

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Molecular mimicry between SARS‐CoV‐2 and the female reproductive system

American Journal of Reproductive Immunology, Journal Year: 2021, Volume and Issue: 86(6)

Published: Aug. 18, 2021

Oogenesis, the process of egg production by ovary, involves a complex differentiation program leading to functional oocytes. This comprises sequential pathway steps that are finely regulated. The question related SARS-CoV-2 infection and fertility has been evoked for several reasons, including mechanism molecular mimicry, which may contribute female infertility generation deleterious autoantibodies, possibly contributing onset an autoimmune disease in infected patients.The immunological potential peptides shared between spike glycoprotein oogenesis-related proteins; Thus we planned systematic study improve our understanding possible effects on using angle mimicry as starting point.A library 82 human proteins linked oogenesis was assembled at random from UniProtKB database oogenesis, uterine receptivity, decidualization, placentation key words. For analyses, artificial polyprotein built joining sequences oogenesis-associated proteins. These were analyzed searching Immune Epitope DataBase immunoreactive epitopes hosting pentapeptides.SARS-CoV-2 found share 41 minimal immune determinants, is, pentapeptides, with 27 relate placentation. All pentapeptides identified, exception four, also present glycoprotein-derived have experimentally validated immunoreactive.

Language: Английский

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Effects of mild/asymptomatic COVID-19 on semen parameters and sex-related hormone levels in men: a systematic review and meta-analysis

Asian Journal of Andrology, Journal Year: 2022, Volume and Issue: 25(3), P. 382 - 388

Published: Aug. 5, 2022

Coronavirus disease 2019 (COVID-19) has yet to be proven alter male reproductive function, particularly in the majority of mild/asymptomatic patients. The purpose this study was explore whether COVID-19 affects semen quality and sex-related hormone levels. To find suitable comparative studies, a systematic review meta-analysis done up January 22, 2022, by using multiple databases (Web Science, PubMed, Embase). Preferred Reporting Items for Systematic Reviews Meta-Analyses (PRISMA) guidelines were used identify choose studies. Meta-analysis examine parameters hormones patients before after infection. effects collection time, fever, intensity verification on following infection also investigated. A total 13 studies (n = 770) included analysis, including three case-control six pre-post four single-arm five showed that with COVID-19, sperm concentration ( I 2 0; P 0.003), count 46.3%; 0.043), progressive motility 50.0%; < 0.001), 76.1%; 0.047), normal morphology 0.001) decreased. Simultaneously, found significant relationship between time infection, inflammation severity, parameter values, fever having only bearing concentration. Furthermore, there no difference levels Mild/asymptomatic had effect short term. It is recommended avoid initiating pregnancy during period time.

Language: Английский

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Association between COVID-19 and Male Fertility: Systematic Review and Meta-Analysis of Observational Studies

The World Journal of Men s Health, Journal Year: 2022, Volume and Issue: 41(2), P. 311 - 311

Published: Oct. 7, 2022

Whether COVID-19 reduces male fertility remains requires further investigation. This meta-analysis and systematic review evaluated the impact of on fertility.The literature in PubMed, Embase, MEDLINE, Web Science, Cochrane Library up to January 01, 2022 was systematically searched, a conducted investigate effect fertility. Totally 17 studies with total 1,627 patients 1,535 control subjects were included our meta-analysis.Regarding sperm quality, decreased count (p=0.012), concentration (p=0.001), motility progressive (p=0.048), viability (p=0.031). Subgroup analyses showed that different group populations did not change results. It found during illness stage COVID-19, semen volume decreased, recovery <90 days. We for ≥90 Fever because significantly reduced motility, without fever days, decreased. Regarding disease severity, moderate type but mild type. sex hormones, increased prolactin estradiol. stage, testosterone (T) levels luteinizing hormone levels. A potential publication bias may have existed meta-analysis.COVID-19 men quality caused disruption. had long-term effects especially motility. is critical conduct larger multicenter determine consequences

Language: Английский

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COVID-19 vaccination does not affect male sexual functions

Reproductive Biology and Endocrinology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Jan. 13, 2023

COVID-19 infection has been linked with erectile dysfunction, which also raised apprehensions about the impact of vaccination on male sexual functions. The purpose this study was to investigate functions, such as function, orgasmic desire, intercourse satisfaction, and overall satisfaction.We used International Index Erectile Function (IIEF) questionnaire for data collection. Mixed methods were adopted study, consisted Google online form distribution hard copies those who not internet friendly. All entered in a spreadsheet scores assigned each response according standard given IIEF questionnaire. Fifteen questions, one corresponding question questionnaire, included assess function.In first part analysis, we calculated function men reporting low (~ 15%) excluded, providing us 465 individuals further analysis. Regarding 71% reported no impact, 3% decline, 2.7% an improvement, 23.3% could impact. We performed analysis basis age-groups participants duration after vaccination, finding that there irrespective age subjects or length period vaccination.COVID-19 does affect including satisfaction.

Language: Английский

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Human semen quality as affected by SARS‐CoV‐2 infection: An up‐to‐date review

Andrologia, Journal Year: 2021, Volume and Issue: 54(2)

Published: Oct. 30, 2021

Revealing the effect of SARS-CoV-2 infection on semen quality is a dynamic priority for many healthcare providers in field as well subfertile patients. Therefore, collective summary this research approach still highly needed, especially with continuous increase original publications. Here, we provide an up-to-date review and discussion to collectively reveal human quality. To do this, reviewed all articles abstracts published Scopus PubMed using keywords 'SARS-CoV-2' 'COVID' versus 'sperm' 'semen'. In summary, it can be revealed that, at both symptomatic recovery stages infection, no investigational evidence shedding semen. Also, mainstream work reveals negative impact parameters, particularly sperm count motility. However, long-term post-recovery comparative studies seem very important particular setting.

Language: Английский

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A dual inhibitor of PIP5K1C and PIKfyve prevents SARS-CoV-2 entry into cells

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(8), P. 1736 - 1749

Published: Aug. 1, 2024

Abstract The SARS-CoV-2 pandemic has had an unprecedented impact on global public health and the economy. Although vaccines antivirals have provided effective protection treatment, development of new small molecule-based antiviral candidates is imperative to improve clinical outcomes against SARS-CoV-2. In this study, we identified UNI418, a dual PIKfyve PIP5K1C inhibitor, as chemical agent that inhibits entry into host cells. UNI418 inhibited proteolytic activation cathepsins, which regulated by PIKfyve, resulting in inhibition cathepsin L-dependent cleavage spike protein its mature form, critical step for viral endosomal escape. We also demonstrated prevented ACE2-mediated endocytosis virus via inhibition. Our results potential targets putative therapeutic compound

Language: Английский

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Ovarian Hyperstimulation Syndrome (OHSS): A Narrative Review and Legal Implications

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(9), P. 915 - 915

Published: Aug. 28, 2024

Background: Infertility is a highly meaningful issue with potentially life-changing consequences, and its incidence has been growing worldwide. Assisted reproductive technology (ART) made giant strides in terms of treating many infertility conditions, despite the risk developing ovarian hyperstimulation syndrome (OHSS), life-threatening complication. Methods: This narrative review draws upon scientific articles found PubMed database. The search spanned 1990–2024 period. Search strings used included “OHSS” or “ovarian hyperstimulation” “IVF” “GnRH” “hCG”; 1098 results were retrieved ultimately narrowed down to 111 suitable sources, i.e., relevant dealing condition’s underlying dynamics, management pathways, evidence-based criteria guidelines, crucial both from clinical perspective standpoint medicolegal tenability. Results: following features constitute OHSS factors: young age, low body weight, polycystic (PCOS), among others. GnRH antagonist can substantially lower severe OHSS, compared long protocol gonadotropin-releasing hormone (GnRH) agonist. However, mild moderate form also possible if used, especially when hCG for final maturation oocytes. For women at agonist trigger freeze-all strategy advisable. one most frequent complications, 30% rate IVF cycles. Conclusion: Providing effective care patients begins early diagnosis, while evaluating comorbidities complications. In addition that, we should pay more attention psychological component this complication as whole. Compliance guidelines best practices essential

Language: Английский

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Impacts of COVID-19 and SARS-CoV-2 on male reproductive function: a systematic review and meta-analysis protocol

BMJ Open, Journal Year: 2022, Volume and Issue: 12(1), P. e053051 - e053051

Published: Jan. 1, 2022

Introduction COVID-19 pandemic caused by SARS-CoV-2 has become a global health challenge. can infect host cells via the ACE2 receptor, which is widely expressed in corpus cavernosum, testis and male reproductive tract, participates erection, spermatogenesis androgen metabolism. Also, immune response persistent fever resulting from may lead to damage of testicular activity, consequently compromising fertility. Methods analysis PubMed, MEDLINE, EMBASE, Web Science, Cochrane Library, China National Knowledge Infrastructure, Science Technology Journal database, Chinese Biomedical Databases Wanfang Data will be systematically searched for observational studies (case–control cohort) published up March 2021 English or literature on impacts function. This protocol follow Preferred Reporting Items Systematic Review Meta-Analysis Protocols guidelines Meta-analysis Observational Studies Epidemiology. The primary outcome semen parameters, additional outcomes include: (a) detection SARS-COV-2 semen, (b) sexual hormones, (c) sperm DNA fragmentation index, (d) erectile function, (e) evaluation also genital tract. Two reviewers independently extract data included based predesigned extraction form. risk bias evaluated through Newcastle-Ottawa Scale studies. Manager software V.5.3 used statistical analysis. Q statistic I² test performed assess heterogeneity among Sensitivity explore robustness pooled effects. We use Grading Recommendations Assessment, Development Evaluation system quality evidence. Ethics dissemination Ethical approval not required results peer-reviewed journal. PROSPERO registration number CRD42021245161.

Language: Английский

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