Hybrid immunity to SARS-CoV-2 arises from serological recall of IgG antibodies distinctly imprinted by infection or vaccination

Cell Reports Medicine, Journal Year: 2024, Volume and Issue: 5(8), P. 101668 - 101668

Published: Aug. 1, 2024

We describe the molecular-level composition of polyclonal immunoglobulin G (IgG) anti-spike antibodies from ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, vaccination, or their combination ("hybrid immunity") at monoclonal resolution. Infection primarily triggers S2/N-terminal domain (NTD)-reactive antibodies, whereas vaccination mainly induces anti-receptor-binding (RBD) antibodies. This imprint persists after secondary exposures wherein >60% ensuing hybrid immunity derives original IgG pool. Monoclonal constituents pool can increase breadth, affinity, and prevalence upon exposures, as exemplified by plasma antibody SC27. Following a breakthrough vaccine-induced SC27 gained neutralization breadth potency against SARS-CoV-2 variants zoonotic viruses (half-maximal inhibitory concentration [IC

Language: Английский

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Deciphering Fc-effector functions against SARS-CoV-2

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(8), P. 756 - 768

Published: Feb. 15, 2024

Language: Английский

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Hybrid immunity to SARS-CoV-2 arises from serological recall of IgG antibodies distinctly imprinted by infection or vaccination

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

We used plasma IgG proteomics to study the molecular composition and temporal durability of polyclonal antibodies triggered by ancestral SARS-CoV-2 infection, vaccination, or their combination ("hybrid immunity"). Infection, whether primary post-vaccination, mainly an anti-spike antibody response S2 domain, while vaccination predominantly induced anti-RBD antibodies. Immunological imprinting persisted after a secondary (hybrid) exposure, with >60% ensuing serological originating from initial generated during first exposure. highlight one instance where hybrid immunity arising breakthrough infection resulted in marked increase breadth affinity highly abundant vaccination-elicited antibody, SC27. With intrinsic binding surpassing theoretical maximum (K

Language: Английский

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An antibody-free evaluation of an mRNA COVID-19 vaccine

Biologicals, Journal Year: 2023, Volume and Issue: 85, P. 101738 - 101738

Published: Dec. 14, 2023

Language: Английский

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