Recent advances in genetic tools for engineering probiotic lactic acid bacteria

Bioscience Reports, Journal Year: 2023, Volume and Issue: 43(1)

Published: Jan. 1, 2023

Synthetic biology has grown exponentially in the last few years, with a variety of biological applications. One emerging applications synthetic is to exploit link between microorganisms, biologics, and human health. To this link, it critical select effective tools for use appropriate microorganisms that would address unmet needs health through development new game-changing by complementing existing technological capabilities. Lactic acid bacteria (LAB) are considered chassis organisms can be genetically engineered therapeutic industrial Here, we have reviewed comprehensively various techniques engineering probiotic LAB strains, such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 mediated genome editing, homologous recombination, recombineering. In addition, also discussed heterologous protein expression systems used LAB. By combining computational genetic engineering, there lot potential develop next-generation capabilities bottlenecks scale-up complex biologics production. Recently, started working on Lactochassis project where aim next generation biomedical application.

Language: Английский

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Efficacy and safety of a synthetic biotic for treatment of phenylketonuria: a phase 2 clinical trial

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(10), P. 1685 - 1690

Published: Sept. 28, 2023

Language: Английский

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Evolving understanding of autoimmune mechanisms and new therapeutic strategies of autoimmune disorders

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Oct. 4, 2024

Autoimmune disorders are characterized by aberrant T cell and B reactivity to the body's own components, resulting in tissue destruction organ dysfunction. diseases affect a wide range of people many parts world have become one major concerns public health. In recent years, there been substantial progress our understanding epidemiology, risk factors, pathogenesis mechanisms autoimmune diseases. Current approved therapeutic interventions for mainly non-specific immunomodulators may cause broad immunosuppression that leads serious adverse effects. To overcome limitations immunosuppressive drugs treating diseases, precise target-specific strategies urgently needed. date, significant advances made immune tolerance, offering new avenue developing antigen-specific immunotherapies These approaches shown great potential various preclinical animal models recently evaluated clinical trials. This review describes common manifestation with focus on typical including multiple sclerosis, type 1 diabetes, rheumatoid arthritis, systemic lupus erythematosus, sjögren's syndrome. We discuss current therapeutics developed this field, highlight use nanomaterials mRNA vaccine techniques induce tolerance.

Language: Английский

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Bifidobacterium animalis BD400 protects from collagen-induced arthritis through histidine metabolism

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 22, 2025

Background Rheumatoid arthritis (RA) is a common chronic and systemic autoimmune disease. Numerous clinical studies have indicated correlation between alterations in gut microbiota the onset progression of RA. This research aims to restore intestinal healthy state through oral administration Bifidobacterium early stages with goal delaying Methods Collagen-induced (CIA) rat model was constructed assess development RA using scores, paw thickness, pathological analysis knee joint. The immune response evaluated by determinating specific antibodies cytokines serum synovial fluid. expression barrier protein analyzed qPCR evaluate function. Alterations metabolites were assessed 16S rDNA non-targeted metabolomics. Results findings reveal that administering animalis BD400 orally led significant reduction scores swelling thickness CIA rats. Additionally, there decrease osteo-facial fusion calcified cartilage thickening Furthermore, B. resulted down-regulation inflammatory factors TNF-α collagenase MMP-13 Levels (anti-CII IgG, anti-CII IgG1, IgG2a) cytokine IL-17A serum, as well (TNF-α IL-1β) fluid BD400-treated rats, significantly reduced ( p < 0.05). gene levels proteins (occludin-1, MUC-2, ZO-1) showed increase 0.05) altered composition microorganisms rats at phylum genus levels, particularly affecting HT002. alleviates down-regulating 1-methyl-L-histidine urocanate histidine metabolism, laying foundation for prevention. Conclusion By HT002 metabolism restored permeability, inhibited response, ultimately slowed down

Language: Английский

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The benefits of Lactiplantibacillus plantarum: from immunomodulator to vaccine vector

Immunology Letters, Journal Year: 2025, Volume and Issue: 272, P. 106971 - 106971

Published: Jan. 5, 2025

Probiotics have been increasingly recognized for positively influencing many aspects of human health. Lactiplantibacillus plantarum (L. plantarum), a non-pathogenic bacterium, previously known as Lactobacillus plantarum, is one the lactic acid bacteria commonly used in fermentation. The probiotic properties L. highlighted its health benefits to humans when consumed adequate amounts. strains primarily enter body orally and alter intestinal microflora modulate immune responses their host; thereby benefiting Furthermore, use vaccine vectors delivering mucosal antigens has shown be promising strategy. These aspects, from Immunomodulation delivery by preclinical settings, are this review. Along these lines, construction recombinant strain expressing B cell multi-peptide, future immunity confer anti-tumor effect targeting Her-2/neu-overexpressing cancers local distal sites, also presented discussed.

Language: Английский

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Lactobacillus paracasei LCQ-1 Exhibits Good Safety and Effectively Prevents Escherichia coli K99-Induced Diarrhea in Mice

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Escherichia coli is a common pathogen causing diarrhea in livestock and poultry, posing serious threat to intestinal health leading significant economic losses the animal husbandry industry. While antibiotics have been used treat diarrhea, their numerous drawbacks led increasing attention on probiotic interventions. In this study, Lactobacillus paracasei LCQ-1 was isolated from fresh cow dung, its vitro vivo safety properties were evaluated. experiments demonstrated that exhibited good antimicrobial activity against K99, along with key characteristics such as acid production, bile salt tolerance, high-temperature resistance, hydrophobicity, cell adhesion. Furthermore, it showed no hemolytic activity, gelatinase or mucin-degrading only decarboxylase for histamine. resistant certain antibiotics, resistance genes detected, indicating favorable profiles. vivo, acute toxicity tests mice revealed signs of poisoning after 14 days high-dose oral administration. A 28-day test different doses did not affect organ indices, cause bacterial translocation, alter blood biochemical parameters, damage ileum. infection protection experiment, administration significantly increased survival rate K99-infected 50–80%. conclusion, excellent both shows promising potential preventing treating K99 infections, possible applications management.

Language: Английский

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Construction and immunogenicity of recombinant Lactiplantibacillus plantarum vaccine candidate expressing SARS-CoV-2 Omicron S1 protein

Animals and zoonoses., Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Probiotic Potential of Lactic Acid Bacteria with Antioxidant Properties in Modulating Health: Mechanisms, Applications, and Future Directions

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106181 - 106181

Published: Feb. 1, 2025

Language: Английский

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Engineered Probiotic-Based Biomaterials for Inflammatory Bowel Disease Treatment

Theranostics, Journal Year: 2025, Volume and Issue: 15(8), P. 3289 - 3315

Published: Feb. 18, 2025

Inflammatory bowel disease (IBD) is a chronic condition affecting the intestines, marked by immune-mediated inflammation. This known for its recurrent nature and challenges it presents in treatment. Recently, probiotic have gained attention as promising alternative to traditional small molecular drugs monoclonal antibody chemotherapies IBD. Probiotic, recognized "living" therapeutic agent, offers targeted treatment with minimal side effects flexibility biological modifications, making them highly effective IBD management. comprehensive review latest advancements engineering probiotic-based materials, ranging from basic mechanisms modification techniques used It delves deep into how produces intestinal environment discusses various strategies enhance probiotic's efficacy, including genetic modifications formulation improvements. Additionally, addresses challenges, practical application conditions, future research directions of therapies treatment, providing insights their feasibility potential clinical implications.

Language: Английский

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Lacticaseibacillus paracasei subsp. paracasei Q- 1 Exhibits Good Safety and Effectively Prevents Escherichia coli K99-Induced Diarrhea in Mice

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Language: Английский

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