The oral–gut microbiome axis in health and disease

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(12), P. 791 - 805

Published: July 22, 2024

Language: Английский

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Differences in diversity and composition of mucosa-associated colonic microbiota in colorectal cancer and non-colorectal cancer in Indonesia

World Journal of Gastroenterology, Journal Year: 2025, Volume and Issue: 31(7)

Published: Jan. 18, 2025

Colorectal cancer is the third most common malignancy and fourth leading cause of cancer-related deaths worldwide. Several studies have shown an association between gut microbiota colorectal cancer. Gut unique can be influenced by geographic factors habits. This study aimed to determine diversity composition colonic mucosal in patients with without To Indonesia. case-control included 59 subjects (35 24 non-colorectal indicated for colonoscopy at Dr. Cipto Mangunkusumo Gastrointestinal Endoscopy Center Fatmawati Hospital. Microbiota examination was performed using 16S rRNA sequencing. Bioinformatics analysis wf-metagenomics pipeline from EPI2Me-Labs (Oxford Nanopore Technologies platform). Patients had a higher median index value on Shannon (3.28 vs 2.82, P > 0.05) lower Simpson (0.050 0.060, 0.05). Significant differences beta were observed genus (P = 0.002) species levels 0.001). Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria dominant phyla. The genera Bacteroides, Campylobacter, Peptostreptococcus, Parvimonas found more frequently cancer, while Faecalibacterium, Haemophilus, Phocaeicola relative abundance Fusobacterium nucleatum, Bacteroides fragilis, Enterococcus faecalis, Campylobacter hominis, faecalis significantly elevated Meanwhile, Faecalibacterium prausnitzii, duncaniae, Prevotella copri commonly exhibit distinct their compared those reviewed approved Ethics Committee Faculty Medicine, Universitas Indonesia (No. KET-1517/UN2.F1/ETIK/PPM.00.02/2023).

Language: Английский

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Metal-organic frameworks derived emerging theranostic platforms

Nano Today, Journal Year: 2024, Volume and Issue: 58, P. 102404 - 102404

Published: July 24, 2024

Language: Английский

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The Microbiome Matters: Its Impact on Cancer Development and Therapeutic Responses

The Journal of Microbiology, Journal Year: 2024, Volume and Issue: 62(3), P. 137 - 152

Published: March 1, 2024

Language: Английский

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Au@Fe₃O₄ Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micra

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Colorectal cancer (CRC) is a common malignancy requiring early screening to improve patient outcomes. Current methods such as colonoscopy and fecal occult blood tests have several limitations including high cost, poor specificity, invasiveness, inconvenience. Recent research has identified specific bacterial communities associated with CRC, notably Parvimonas micra (P. micra), which serves biomarker for diagnosis owing its accumulation in the malignant tissues feces of CRC patients. Herein, we employed whole-bacterium systematic evolution ligands by exponential enrichment (SELEX) method isolate high-affinity aptamers against P. using 17 selection cycles. These were subsequently bound Au@Fe3O4 nanoparticles, interaction inhibited peroxidase-like activity thereby blocking 3,3',5,5'-tetramethylbenzidine (TMB) chromogenic reaction resulting measurable reduction absorbance. This colorimetric detection strategy demonstrated linear response across range 100-108 CFU/mL limit 11 CFU/mL. Using aptasensor, assessed abundance clinical samples found significantly higher levels patients compared that healthy individuals, was consistent quantitative polymerase chain results. study therefore represents first successful identification an aptamer affinity specificity micra, leading development highly sensitive aptasensor detection. The presented approach significant potential diagnosis.

Language: Английский

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Novel Therapeutic Strategies and Recent Advances in Gut Microbiota Synergy with Nanotechnology for Colorectal Cancer Treatment

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101601 - 101601

Published: Feb. 20, 2025

Over the last two decades, molecular biology advances have revealed gut microbiota's active and crucial role in colorectal cancer (CRC) pathogenesis its dysregulation during tumorigenesis. This understanding has underscored urgency of exploring novel therapeutic approaches to address challenges posed by CRC. Among these approaches, nanosynergistic therapies, with their potential modulate microbiota, increase drug bioavailability stability, reduce side effects, emerged as a promising avenue. Furthermore, efficacy nanotechnology-based can be enhanced combining them different methods, including chemotherapy, radiotherapy, immunotherapy, photothermal therapy, sonodynamic therapy. Herein, recent progress nano synergistic therapy been reviewed, focusing on synergy between microbiota nanotechnology CRC treatment. Additionally, diverse applications nanoparticles analyzed provide innovative ideas effective solutions for developing highly treatment strategies.

Language: Английский

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Fecal bacterial biomarkers and blood biochemical indicators as potential key factors in the development of colorectal cancer

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

ABSTRACT The incidence of colorectal cancer (CRC) has been increasing in recent decades. Current methods for CRC screening have their own drawbacks, thus there is an urgent need to identify the key microbes that drive development wider application early detection and prevention CRC. To address this issue, we performed fecal microbiome analysis by high-throughput sequencing 16S rRNA gene combined with blood biochemical indicators patients stages I, II, III, IV, healthy people, polyps. Fecal microbiota was disturbed, as evidenced significantly reduced α-diversity stage IV markedly different β-diversity. random forest model identified top 25 genera from 174 training data, resulting a diagnostic accuracy 87.95%. Further, combining differential analysis, screened out 11 biomarkers changed groups. Peptostreptococcus , Parvimonas Shewanella Oscillibacter Eggerthella Gemella associated were enriched, while Fenollaria Staphylococcus Ezakiella Finegoldia, Neisseria remission suppressed Importantly, carcinoembryonic antigen (CEA) correlated these microbial biomarkers, carbohydrate 19-9 (CA 19-9) . Notably, co-occurrence network at genus level exhibited most complex stable. These results suggested CEA, CA may be co-biomarkers disease occurrence development, non-invasive diagnosis IMPORTANCE Identifying important field. We delved into research on association between study, providing detailed characteristics during transition normal intestine polyps cancer. bacterial

Language: Английский

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The Oral Microbiome and Cancer

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown, P. 151 - 170

Published: Jan. 1, 2025

Language: Английский

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Poor prognosis in IBD-complicated colon cancer through gut dysbiosis-related immune response failure

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Summary Background Colorectal cancer (CRC) results from the accumulation of mutations and epigenetic changes in gut epithelial cells likely due to microbiota dysbiosis. However, limited research has been done explore link between host tumour dysbiosis disease outcome. Methods The mechanisms influencing outcomes 97 colorectal patients, including 13 with Lynch syndrome, 20 inflammatory bowel (IBD), 64 sporadic cases, were analyzed using a multiomics approach. These patients categorized into two groups: “disease-free/stable disease” “progression survival outcomes. analysis included tumor adherent composition (16S rRNA), somatic gene (WES), expression (RNAseq), immune markers (RNAscope), infiltrate (immunohistochemistry). Results IBD-CRC had worse than those or CRC, regardless TNM staging treatment. Symbiotic bacteria like Lactococcus lactis significantly reduced tissues. Patient influenced by abundance virulent ( Escherichia coli ) relative beneficial ). Although no significant increase deleterious was found IBD-CRC. 16sRNA revealed increased virulent- decreased anti-inflammatory symbiotic-bacteria correlating upregulation oncogenes downregulation anti-oncogenes PHLPP1. multiplex situ hybridization CD8, IFNγ PHLPP1 an anti-oncogene decrease detectable tissues as compared CRCs. Conclusion poor are cell alterations, possibly triggered microbiota-related pathways. What You Need Know BACKGROUND AND CONTEXT is associated Inflammatory disease-related CRC (IBD-CRC) classified environment-related condition. NEW FINDINGS In relation patient outcomes, three types (Sporadic-, IBD-, syndrome-CRC) multiomic This examining tissue bacteria, analyses, quantifying infiltration mucosa. worst down regulation gene, virulent/symbiotic imbalance, response failure. LIMITATIONS Lack animal experiments FMT fresh stool patients. CLINICAL TRANSLATIONAL RESEARCH RELEVANCE Among different showed greater imbalance harmful along Lay summary study compares pathological clinical characteristics across distinct etiologies: (IBD)-associated syndrome-associated (LS-CRC). Distinct differences tumor-adherent microbiota, profiles observed. Notably, demonstrated poorest prognosis depending on microbe-host interaction highlighting potential biomarkers for treatment strategies.

Language: Английский

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TiO2-Coated Honeycomb-Like Super-Macroporous Silica for High-Purity Extraction of Exosomes from Human Plasma

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128098 - 128098

Published: April 1, 2025

Language: Английский

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