Development of SARS‐CoV‐2 animal vaccines using a stable and efficient NDV expression system DOI

Lei He,

Jiaying Zhong,

Guichang Li

et al.

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 95(1)

Published: Oct. 19, 2022

With the continuation of coronavirus disease 2019 pandemic and emergence new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants, control spread virus remains urgent. Various animals, including cats, ferrets, hamsters, nonhuman primates, minks, tree shrews, fruit bats, rabbits, are susceptible to SARS-CoV-2 infection naturally or experimentally. Therefore, avoid animals from becoming mixing vessels virus, vaccination should be considered. In present study, we report establishment an efficient stable system using Newcastle (NDV) as a vector express spike protein/subunit for rapid generation vaccines against in animals. Our data showed that S S1 protein was sufficiently expressed rNDV-S rNDV-S1-infected cells, respectively. The incorporated into displayed on surface viral particles. Intramuscular immunization with found induce highest level binding neutralizing antibodies, well strong S-specific T-cell response mice. Intranasal rNDV-S1 provoked robust but barely any detectable antibodies. Overall, NDV-vectored vaccine candidates were able profound humoral cellular immunity, which will provide good developing targeting both antibody responses.

Language: Английский

Transmission of SARS-CoV-2 delta variant (AY.127) from pet hamsters to humans, leading to onward human-to-human transmission: a case study DOI Creative Commons
Hui‐Ling Yen,

Thomas H. C. Sit,

Christopher J. Brackman

et al.

The Lancet, Journal Year: 2022, Volume and Issue: 399(10329), P. 1070 - 1078

Published: March 1, 2022

Language: Английский

Citations

177
Multiple Introductions of SARS-CoV-2 Alpha and Delta Variants into White-Tailed Deer in Pennsylvania DOI Creative Commons
Andrew D. Marques, Scott Sherrill-Mix, J.K. Everett

et al.

mBio, Journal Year: 2022, Volume and Issue: 13(5)

Published: Aug. 24, 2022

Coronaviruses have been documented to replicate in numerous species of vertebrates, and multiple spillovers coronaviruses from animals into humans founded human epidemics. The COVID-19 epidemic likely derived a spillover SARS-CoV-2 bats humans, possibly via an intermediate host.

Language: Английский

Citations

57
Widespread exposure to SARS-CoV-2 in wildlife communities DOI Creative Commons
Amanda R. Goldberg, Kate E. Langwig, Katherine L. Brown

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 29, 2024

Pervasive SARS-CoV-2 infections in humans have led to multiple transmission events animals. While has a potential broad wildlife host range, most documented been captive animals and single species, the white-tailed deer. The full extent of exposure among communities factors that influence risk remain unknown. We sampled 23 species for examined effects urbanization human use on seropositivity. Here, we document positive detections RNA six including deer mouse, Virginia opossum, raccoon, groundhog, Eastern cottontail, red bat between May 2022-September 2023 across Washington, D.C., USA. In addition, found sites with high activity had three times higher seroprevalence than low human-use areas. obtained genomic sequences from nine individuals which were assigned seven Pango lineages Omicron variant. close match variants circulating at time suggests least recent human-to-animal events. Our data support widespread areas may serve as points contact cross-species transmission.

Language: Английский

Citations

15
Cats and SARS-CoV-2: A Scoping Review DOI Creative Commons
Ramona Doliff, Pim Martens

Animals, Journal Year: 2022, Volume and Issue: 12(11), P. 1413 - 1413

Published: May 30, 2022

Since the beginning of COVID-19 pandemic, various animal species were found to be susceptible SARS-CoV-2 infection. The close contact that exists between humans and cats warrants special attention role this species. Therefore, a scoping review was performed obtain comprehensive overview existing literature, map key concepts, types research, possible gaps in research. A systematic search databases PubMed, Google Scholar, Scopus preprint servers medRxiv bioRxiv performed. After two-step screening process, 27 peer-reviewed articles, 8 scientific communication items, 2 unpublished pre-prints included. main themes discussed susceptibility SARS-CoV-2, induced immunity, prevalence infection, manifestation interspecies transmission cats, lastly, intraspecies cats. research identified lack large-scale studies, underrepresentation stray, feral, shelter cat populations, investigation into cat-to-cat transmissions under non-experimental conditions, relation other regarding SARS-CoV-2. Overall, seemingly play limited spread While are virus reverse zoonotic from happens regularly, there is currently no evidence circulation among

Language: Английский

Citations

30
First serological evidence of SARS-CoV-2 natural infection in small ruminants DOI Creative Commons
Giovanna Fusco, Lorena Cardillo,

Martina Levante

et al.

Veterinary Research Communications, Journal Year: 2023, Volume and Issue: 47(3), P. 1741 - 1748

Published: Jan. 10, 2023

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) emerged in late December 2019 and spread worldwide, quickly becoming a pandemic. This zoonotic coronavirus shows broad host range, including wildlife domestic animals. Small ruminants are shown to be susceptible SARS-CoV-2 but, date, no natural infection has been reported. Herein, we performed survey for among sheep goats the Campania region of Italy using an indirect multispecies ELISA. Next, positive sera were submitted virus serum neutralization quantification specific neutralizing antibodies. Out 612 goats, 23 found ELISA (3.75%) 1 them showed 1:20 antibodies titer. No significant difference was between two species, as well male female, geographical location age. Our findings demonstrate that can occur flocks field situation. Moreover, low susceptibility is reported nevertheless, continuous mutations this open new scenarios on viral range tropism, highlighting importance investigating animal species could represent ongoing or future possible hosts.

Language: Английский

Citations

15
When COVID-19 sits on people's laps: A systematic review of SARS-CoV-2 infection prevalence in household dogs and cats DOI Creative Commons

Ruoshui Guo,

Cecilia Wolff, Joaquín M. Prada

et al.

One Health, Journal Year: 2023, Volume and Issue: 16, P. 100497 - 100497

Published: Feb. 3, 2023

During the COVID-19 pandemic, questions were raised about whether SARS-CoV-2 can infect pets and potential risks posed to by their human owners. We performed a systematic review of studies on infection prevalence in naturally infected household dogs cats conducted worldwide published before January 2022. Data prevalence, as determined either molecular or serological methods, accompanying information, summarized. Screening targeting general dog cat populations differentiated from those households with known COVID-19-positive people. Studies focusing stray, sheltered working animals excluded. In total, 17 included this review. Fourteen investigated cats, 13 dogs, 10 both. Five reported 16 seroprevalence, four All but two started ended 2020. eight European countries (Italy, France, Spain, Croatia, Germany, Netherlands, UK, Poland), three Asian (Iran, Japan, China) USA. Both pet population usually below 5%, exceeded 10% when positive people be present household. A meta-analysis provided pooled seroprevalence estimates population: 2.75% (95% Confidence Interval [CI]: 1.56-4.79%) 0.82% CI: 0.26-2.54%) for respectively. This highlighted need better understanding possible epizootic implications well global standards detection pets.

Language: Английский

Citations

13
DETECTION OF SARS-COV-2 IN A SQUIRREL MONKEY (SAIMIRI SCIUREUS): A ONE HEALTH INVESTIGATION AND RESPONSE DOI Creative Commons
Hayley D. Yaglom, Alexis M. Roth, Carolina Álvarez

et al.

Journal of Zoo and Wildlife Medicine, Journal Year: 2024, Volume and Issue: 55(2)

Published: June 13, 2024

Through collaborative efforts, One Health partners have responded to outbreaks of COVID-19 among animals, including those in human care at zoos. Zoos been faced with numerous challenges, the susceptibility many mammalian species, and therefore need heighten biosecurity measures rapidly. Robust collaborations already exist Arizona address endemic emerging zoonoses, but these rarely included The pandemic shed light on this, subsequently expanded its SARS-CoV-2 surveillance efforts include zoo animals. Testing epidemiologic support was provided expedite detection response zoonotic infection as well understand possible transmission events. Resulting from this program, detected a rectal swab collected an 8-yr-old squirrel monkey (Saimiri sciureus) Southern Arizona. animal had rapidly become ill nonrespiratory symptoms died July 2022. Genomic sequencing revealed mutations consistent Omicron (BA.2) lineage. An investigation identified caretaker close proximity affected who tested positive for same day died. Critical through engagement local, state, federal agencies. Necropsy pathologic evaluation showed significant necrotizing colitis; overall clinical histopathological findings did not implicate alone causal or contributing factor monkey's illness death. This report documents first identification highlights successful timely conducted multisectoral collaboration.

Language: Английский

Citations

3
Possible spread of SARS-CoV-2 in domestic and wild animals and body temperature role DOI Creative Commons
Zinaida Klestova

Virus Research, Journal Year: 2023, Volume and Issue: 327, P. 199066 - 199066

Published: Feb. 10, 2023

The COVID-19 pandemic was officially announced in March 2020 and is still moving around the world. Virus strains, their pathogenicity infectivity are changing, but ability fast to spread harm people's health remained, despite seasonality seasons other circumstances. Most likely, humanity doomed for a long time coexistence with this emergent pathogen, since it already circulating not only among human population, fauna, especially wild animals different regions of planet. Thus, range virus has expanded, material conditions its evolution more than enough. detection SARS-CoV-2 known infected fauna species analyzed possible ongoing circulation domestic discussed. One main focus article role animal body temperature, fluctuations presence entry receptors susceptibility infection spreading new ecological niches. possibility long-term pathogen susceptible organisms

Language: Английский

Citations

8
Widespread exposure to SARS-CoV-2 in wildlife communities DOI Creative Commons
Amanda R. Goldberg, Kate E. Langwig, Katherine L. Brown

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 7, 2022

Abstract Pervasive SARS-CoV-2 infections in humans have led to multiple transmission events captive animals. While has a potential broad wildlife host range, most documented date are found single species, the white-tailed deer. The extent of exposure among species and factors that influence risk remain unknown. We sampled 23 for examined effects urbanization human use on seropositivity. Here, we document positive detections RNA six including deer mouse, Virginia opossum, raccoon, groundhog, Eastern cottontail, red bat. In addition, sites with high activity had three times higher seroprevalence than low human-use areas. detected genomic sequences from nine individuals which were assigned seven Pango lineages Omicron variant. close match variants circulating at time suggests least recent human-to-animal events. Our data support been widespread communities areas may serve as points contact cross-species transmission.

Language: Английский

Citations

14
SARS-CoV-2 Serological and Biomolecular Analyses among Companion Animals in Campania Region (2020–2021) DOI Creative Commons
Lorena Cardillo, Claudio de Martinis, Sergio Brandi

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(2), P. 263 - 263

Published: Jan. 24, 2022

The first reports of SARS-CoV-2 among domestic and wild animals, together with the rapid emergence new variants, have created serious concerns regarding a possible spillback from animal hosts, which could accelerate evolution viral strains. present study aimed to investigate prevalence transmission both owned stray pets. A total 182 dogs 313 cats were tested for SARS-CoV-2. Specimens collected pets subjected RT-PCR serological examinations. No RNA was detected, while anti-N antibodies observed in six animals (1.3%), one dog (0.8%) five (1.7%). Animals' background revealed that cats, living owners COVID-19, showed significantly different compared ones (p = 0.0067), no difference found dogs. Among seropositive pets, three also moderate neutralizing antibody titers. Pets other species are susceptible infection because spike affinity towards their ACE2 cellular receptor. Nevertheless, risk retransmission remains unclear since pet-to-human has never been described. Due virus' high mutation rate, reservoirs cannot be excluded; thus, it is reasonable test mostly if households affected by COVID-19.

Language: Английский

Citations

13