Development of SARS‐CoV‐2 animal vaccines using a stable and efficient NDV expression system DOI

Lei He,

Jiaying Zhong,

Guichang Li

et al.

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 95(1)

Published: Oct. 19, 2022

With the continuation of coronavirus disease 2019 pandemic and emergence new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants, control spread virus remains urgent. Various animals, including cats, ferrets, hamsters, nonhuman primates, minks, tree shrews, fruit bats, rabbits, are susceptible to SARS-CoV-2 infection naturally or experimentally. Therefore, avoid animals from becoming mixing vessels virus, vaccination should be considered. In present study, we report establishment an efficient stable system using Newcastle (NDV) as a vector express spike protein/subunit for rapid generation vaccines against in animals. Our data showed that S S1 protein was sufficiently expressed rNDV-S rNDV-S1-infected cells, respectively. The incorporated into displayed on surface viral particles. Intramuscular immunization with found induce highest level binding neutralizing antibodies, well strong S-specific T-cell response mice. Intranasal rNDV-S1 provoked robust but barely any detectable antibodies. Overall, NDV-vectored vaccine candidates were able profound humoral cellular immunity, which will provide good developing targeting both antibody responses.

Language: Английский

Equine Coronaviruses DOI Open Access
Nicola Pusterla

Veterinary Clinics of North America Equine Practice, Journal Year: 2022, Volume and Issue: 39(1), P. 55 - 71

Published: Nov. 21, 2022

Language: Английский

Citations

6
SARS-CoV-2 in companion animals: Do levels of SARS-CoV-2 seroconversion in pets correlate with those of pet’s owners and with protection against subsequent SARS-CoV-2 infection? DOI Creative Commons
Hannah Murphy,

Shania Sanchez,

Shamim Ahmed

et al.

Virulence, Journal Year: 2022, Volume and Issue: 13(1), P. 1216 - 1220

Published: July 8, 2022

Language: Английский

Citations

5
Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020 DOI Creative Commons
Taylor Estes Gin, Elizabeth Petzold, Diya M. Uthappa

et al.

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e16310 - e16310

Published: Oct. 24, 2023

We collected oral and/or rectal swabs and serum from dogs cats living in homes with SARS-CoV-2-PCR-positive persons for SARS-CoV-2 PCR serology testing. Pre-COVID-19 samples were used as negative controls, tested duplicate at different timepoints. Raw ELISA results scrutinized relative to known suggested that cut-offs IgG seropositivity may require adjustment previously proposed values, while IgM more extensive validation. A small number of pet (2/43, 4.7%) one cat (1/21, 4.8%) positive RNA, 28.6 37.5% anti-SARS-CoV-2 IgG, respectively.

Language: Английский

Citations

2
What are the risk levels of humans contracting SARS‐CoV‐2 from pets and vice versa? DOI
Hannah Murphy, Hinh Ly

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 94(12), P. 5613 - 5614

Published: July 30, 2022

Language: Английский

Citations

4
Development of SARS‐CoV‐2 animal vaccines using a stable and efficient NDV expression system DOI

Lei He,

Jiaying Zhong,

Guichang Li

et al.

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 95(1)

Published: Oct. 19, 2022

With the continuation of coronavirus disease 2019 pandemic and emergence new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants, control spread virus remains urgent. Various animals, including cats, ferrets, hamsters, nonhuman primates, minks, tree shrews, fruit bats, rabbits, are susceptible to SARS-CoV-2 infection naturally or experimentally. Therefore, avoid animals from becoming mixing vessels virus, vaccination should be considered. In present study, we report establishment an efficient stable system using Newcastle (NDV) as a vector express spike protein/subunit for rapid generation vaccines against in animals. Our data showed that S S1 protein was sufficiently expressed rNDV-S rNDV-S1-infected cells, respectively. The incorporated into displayed on surface viral particles. Intramuscular immunization with found induce highest level binding neutralizing antibodies, well strong S-specific T-cell response mice. Intranasal rNDV-S1 provoked robust but barely any detectable antibodies. Overall, NDV-vectored vaccine candidates were able profound humoral cellular immunity, which will provide good developing targeting both antibody responses.

Language: Английский

Citations

4