GMOs or non-GMOs? The CRISPR Conundrum DOI Creative Commons
Aftab Ahmad, Amer Jamil, Nayla Munawar

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 9, 2023

CRISPR-Cas9, the “genetic scissors”, is being presaged as a revolutionary technology, having tremendous potential to create designer crops by introducing precise and targeted modifications in genome achieve global food security face of climate change increasing population. Traditional genetic engineering relies on random unpredictable insertion isolated genes or foreign DNA elements into plant genome. However, CRISPR-Cas based gene editing does not necessarily involve inserting element from different species but new traits precisely altering existing genes. CRISPR edited are touching markets, however, world community divided over whether these should be considered genetically modified (GM) non-GM. Classification crops, especially transgene free traditional GM will significantly affect their future public acceptance some regions. Therefore, depending upon regulation non-GMs, perception. Here we briefly discuss how crops. In addition, reagents delivery tools produce transgene-free Moreover, also summarize regulatory classification countries regulating We that controversy CRISPR-edited plants non-GM continue until universal, transparent, scalable framework for introduced worldwide, with increased awareness involving all stakeholders.

Language: Английский

Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers DOI

Seon‐Yeong Kwak,

Tedrick Thomas Salim Lew, Connor J. Sweeney

et al.

Nature Nanotechnology, Journal Year: 2019, Volume and Issue: 14(5), P. 447 - 455

Published: Feb. 25, 2019

Language: Английский

Citations

477
In vitro plant tissue culture: means for production of biological active compounds DOI Open Access
Claudia A. Espinosa-Leal, César A. Puente-Garza, Silverio García‐Lara

et al.

Planta, Journal Year: 2018, Volume and Issue: 248(1), P. 1 - 18

Published: May 7, 2018

Language: Английский

Citations

468
Nanoparticle-Mediated Delivery towards Advancing Plant Genetic Engineering DOI
F. J. Cunningham, Natalie S. Goh, Gözde S. Demirer

et al.

Trends in biotechnology, Journal Year: 2018, Volume and Issue: 36(9), P. 882 - 897

Published: April 24, 2018

Language: Английский

Citations

370
Improved drought stress tolerance in Arabidopsis by CRISPR/dCas9 fusion with a Histone AcetylTransferase DOI Creative Commons
Joaquin Felipe Roca Paixão, François‐Xavier Gillet, Thuanne Pires Ribeiro

et al.

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: May 30, 2019

Abstract Drought episodes decrease plant growth and productivity, which in turn cause high economic losses. Plants naturally sense respond to water stress by activating specific signalling pathways leading physiological developmental adaptations. Genetically engineering genes that belong these might improve the drought tolerance of plants. The abscisic acid (ABA)-responsive element binding protein 1/ABRE factor (AREB1/ABF2) is a key positive regulator response. We investigated whether CRISPR activation (CRISPRa) system targets AREB1 contribute Arabidopsis. Arabidopsis histone acetyltransferase 1 (AtHAT1) promotes gene expression switching chromatin relaxed state. Stable transgenic plants expressing chimeric dCas9 HAT were first generated. Then, we showed CRISPRa mechanism increased promoter activity controlling β-glucuronidase ( GUS ) reporter gene. To activate endogenous , was set up, resultant dwarf phenotype. Our qRT-PCR experiments indicated both RD29A positively regulated AREB1, exhibited higher than control generated here chlorophyll content faster stomatal aperture under deficit, addition better survival rate after stress. Altogether, report valuable biotechnological tool through regulation AREB1.

Language: Английский

Citations

231
The genome editing revolution: review DOI Creative Commons
Ahmad M. Khalil

Journal of Genetic Engineering and Biotechnology, Journal Year: 2020, Volume and Issue: 18(1), P. 68 - 68

Published: Oct. 29, 2020

Development of efficient strategies has always been one the great perspectives for biotechnologists. During last decade, genome editing different organisms a fast advancing field and therefore received lot attention from various researchers comprehensively reviewing latest achievements offering opinions on future directions. This review presents brief history, basic principles, advantages disadvantages, as well aspects each technology including modes, applications, challenges that face delivery gene components. Genetic modification techniques cover wide range studies, generation transgenic animals, functional analysis genes, model development diseases, or drug development. The certain proteins such monoclonal antibodies, enzymes, growth hormones suffering several obstacles because their large size. These difficulties encouraged scientists to explore alternative approaches, leading progress in editing. distinguished efforts enormous experimentation have now able introduce methodologies can change genetic constitution living cell. evolved during three decades, nowadays, four types "programmable" nucleases are available this field: meganucleases, zinc finger nucleases, transcription activator-like effector clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) (CRISPR/Cas-9) system. Each group its own characteristics necessary select most suitable method tool applications. Genome engineering/editing will revolutionize creation precisely manipulated genomes cells order modify specific characteristic. Of potential applications those human health agriculture. Introducing constructs into target is key step engineering. Despite success already achieved, still difficulties. Challenges must be overcome before full realized.

Language: Английский

Citations

196
Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration DOI Creative Commons
Siamak Shirani Bidabadi, S. Mohan Jain

Plants, Journal Year: 2020, Volume and Issue: 9(6), P. 702 - 702

Published: June 1, 2020

Plants generally have the highest regenerative ability because they show a high degree of developmental plasticity. Although basic principles plant regeneration date back many years, understanding cellular, molecular, and physiological mechanisms based on these is currently in progress. In addition to significant effects some factors such as medium components, phytohormones, explant type, light an explant, recent reports evidence involvement molecular signals organogenesis embryogenesis responses wounding, induced cell death, phytohormones interaction. However, cellular behaviors occurrence somaclonal variations abnormalities during vitro process may be associated with adverse efficacy regeneration. A review past studies suggests that, cases, plants involves reprogramming distinct somatic cells, while others, it by activation relatively undifferentiated cells tissues. this covers most important involved discusses which monitor process.

Language: Английский

Citations

190
Genetic engineering of microalgae for enhanced biorefinery capabilities DOI

Mehmooda Fayyaz,

Kit Wayne Chew, Pau Loke Show

et al.

Biotechnology Advances, Journal Year: 2020, Volume and Issue: 43, P. 107554 - 107554

Published: May 11, 2020

Language: Английский

Citations

164
Plants’ Response to Abiotic Stress: Mechanisms and Strategies DOI Open Access
Yan Zhang, Jing Xu, Ruofan Li

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 10915 - 10915

Published: June 30, 2023

Abiotic stress is the adverse effect of any abiotic factor on a plant in given environment, impacting plants' growth and development. These factors, such as drought, salinity, extreme temperatures, are often interrelated or conjunction with each other. Plants have evolved mechanisms to sense these environmental challenges make adjustments their order survive reproduce. In this review, we summarized recent studies sensing its regulatory mechanism, emphasizing signal transduction regulation at multiple levels. Then presented several strategies improve under based current progress. Finally, discussed implications research response stresses for high-yielding crops agricultural sustainability. Studying signaling critical understand responses plants generate stress-resistant

Language: Английский

Citations

128
Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants DOI Creative Commons
Manoj Kumar, Manas Ranjan Prusty, Manish K. Pandey

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: April 18, 2023

Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery engineered nucleases as genetic scissors carry out precise manipulation in crop stress-responsive genes associated molecular network has paved way for sustainable management abiotic stress conditions. In this context, clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool revolutionized due its simplicity, accessibility, adaptability, flexibility, wide applicability. This system great potential build up varieties with enhanced tolerance against review, we summarize latest findings on understanding mechanism response plants application CRISPR/Cas-mediated towards a multitude stresses metals. We provide mechanistic insights CRISPR/Cas9-based genome editing technology. also discuss applications evolving techniques such prime base editing, mutant library production, transgene free multiplexing rapidly deliver modern cultivars adapted

Language: Английский

Citations

81
Biofortification of major crop plants with iron and zinc - achievements and future directions DOI Creative Commons
James Stangoulis, Marija Knez

Plant and Soil, Journal Year: 2022, Volume and Issue: 474(1-2), P. 57 - 76

Published: Feb. 17, 2022

Abstract Biofortification is a long-term strategy of delivering more iron (Fe) and zinc (Zn) to those most in need. Plant breeding programs within the CGIAR NARS have made major advances Fe- Zn-dense variety development there been successful releases new biofortified varieties. Recent research effort has led substantial improvement our knowledge Fe Zn homeostasis gene regulation, resulting identification candidate genes for marker assisted selection. International cooperation between agricultural nutrition community strengthened, with numerous implementation partnership strategies developed employed over years. The evidence on effectiveness crops slowly building up results are encouraging. continues be scaled out further work required reach general aim eradicating hidden hunger deficiency world’s population ensuring nutritional security.

Language: Английский

Citations

70