Journal of Neonatal-Perinatal Medicine, Journal Year: 2024, Volume and Issue: 17(5), P. 661 - 671
Published: Oct. 8, 2024
Late-onset neonatal sepsis has a high mortality rate in premature infants. To date, no single test the evaluation of been demonstrated to be both sensitive and specific enough assist timely decision making. The aim our study is develop predictive model that can applied all babies, using clinical laboratory findings recognize late-onset sepsis.
Language: Английский
Journal of Clinical Laboratory Analysis, Journal Year: 2024, Volume and Issue: 38(3)
Published: Jan. 24, 2024
Abstract Background Procalcitonin (PCT) is a critical biomarker that released in response to bacterial infections and can be used differentiate the pathogenesis of infectious process. Objective In this article, we provide an overview recent advances PCT biosensors, highlighting different approaches for biosensor construction, immobilization methods, advantages roles matrices used, analytical performance, construction. Also, will explain biosensors sensible limits detection (LOD), linearity, other characteristics. Future prospects development better systems are also discussed. Methods Traditional methods such as capillary electrophoresis, high‐performance liquid chromatography, mass spectrometry effective analyzing medical field, but they complicated, time‐consuming sample preparation, require expensive equipment skilled personnel. Results past decades, have emerged simple, fast, sensitive tools analysis various fields, especially fields. Conclusion These potential accompany or replace traditional by simplifying reducing preparation making field testing easier faster, while significantly cost per analysis.
Language: Английский
Citations
7
PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0314702 - e0314702
Published: Feb. 12, 2025
Sepsis is the overwhelming immunological response to infection, which if not treated can lead multi-organ failure, shock and death. Specifically, neonatal sepsis results in 225,000 deaths globally per annum. Moreover, Uganda experiences one of highest materno-fetal death rates (62,000 p.a.), with at approximately 6,500 p.a.. The difficulty diagnosing lies non-specific signs symptoms associated an absence definitive sepsis-specific biomarkers. However, serum amyloid A (SAA) detection has potential as a superior biomarker for diagnosis probable sepsis. Herein, ethically-approved studies we have deployed competitive lateral flow test (NeoSep-SAA (research-use only)) detect SAA whole blood patient bedside resource-limited environment. Results are available within 10 minutes format compatible small volumes from neonates (5 μl). NeoSep-SAA exhibited high sensitivity specificity adult sepsis, showed 92% (89%, 95%) 73% (68%, 77%) PPV NPV 78% (75%, 81%) 90% (86%, 93%), respectively (n = 714 individuals; 95% CI). over C-Reactive Protein (sensitivity: 37%), albeit some sacrifice specificity. enabled rapid diagnosis, combined minimally-invasive withdrawal, was less stressful neonates. Overall, readily identify infection/inflammation enable informed clinical decisions combat This approach improve reduce mortality line United Nations Sustainable Development Goal (SDG) 3.2 objectives.
Language: Английский
Citations
0
Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 11
Published: Jan. 9, 2025
This study aimed to investigate the association between serum heat shock protein 27 (HSP27) levels and 28-day mortality in patients with sepsis. retrospective analyzed clinical data of 76 septic admitted intensive care unit (ICU). Fifty non-septic ICU 50 healthy individuals served as control groups. Serum HSP27 were measured on day admission compared sepsis severity survival outcomes. Median (4.70 ng/mL, IQR: 2.10-13.48 ng/mL) significantly higher than those both controls (all p < 0.05). Moreover, non-survivors exhibited median (9.30 3.62-25.91 survivors (3.03 1.48-7.39 Multivariate logistic regression analysis confirmed patients. Receiver operating characteristic (ROC) curve revealed an area under (AUC) 0.720 (95% CI: 0.605-0.817, 0.001) for predicting prognosis. Survival demonstrated that high (≥2.61 had a worse prognosis low (<2.61 ng/mL). shows potential biomarker diagnosis sepsis, however, further research is necessary solidify its utility.
Language: Английский
Citations
0
Antibiotics, Journal Year: 2025, Volume and Issue: 14(1), P. 101 - 101
Published: Jan. 17, 2025
Background: Neonatal sepsis is a serious condition with high mortality, especially in premature and low-birth-weight neonates. This study aims to examine whether the haemostatic profile of neonates defers depending on type bacteria (Gram-positive or Gram-negative), by using method Rotational Thromboelastometry (ROTEM). Methods: single-centre prospective cohort was conducted 128 sepsis, including 95 cases caused Gram-negative pathogens 33 Gram-positive bacteria. All participants were hospitalised Intensive Care Unit (NICU). ROTEM parameters compared between infections. Results: The found be significantly different suffering from versus infections, associated an increased clotting potential pathogens. reflected higher values such as A10, α-angle, MCF EXTEM INTEM assays. Multivariant analysis showed that infections linked clot thickness at 10 min (coefficient: 8.9, CI: 2.8-15.0, p = 0.004), maximum stability 10.4, 4.3-16.6, 0.001), bigger α-angle 8.0, 2.7-13.2, 0.003). Similar findings observed assay parameters. Conclusions: leads hypercoagulable state, whereas exhibit more hypocoagulable incidence haemorrhagic episodes. These provide valuable insights into disorders may aid developing individualised approach for treatment those disorders, dependent adapted specific causative organism.
Language: Английский
Citations
0
Antibiotics, Journal Year: 2024, Volume and Issue: 13(9), P. 877 - 877
Published: Sept. 13, 2024
Neonatal infectious disease continues to result in high rates of infant morbidity and mortality. Early- late-onset represent difficult detect treat illnesses, particularly when antimicrobial resistant pathogens are present. Newborns immunodeficient at increased risk vertical horizontal infection, with preterm infants increasingly susceptible. Additional factors associated infection include prolonged use a central catheter and/or ventilation, congenital abnormalities, admittance intensive care units, the broad-spectrum antibiotics. There is increasing recognition importance host microbiome dysbiosis on neonatal disease, including necrotising enterocolitis sepsis patients. Current diagnostic methods rely blood culture, which unreliable, time consuming, can false negatives. lack accurate reliable tools available for early detection infants; therefore, efficient triage treatment remains challenging. The application biomarkers, machine learning, artificial intelligence, biosensors, microfluidics technology, may offer improved methodologies. Point-of-care devices, such methodologies, provide fast, reliable, aids This review will discuss as impacted by resistance highlight novel point-of-care options.
Language: Английский
Citations
3
Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)
Published: July 2, 2024
Abstract Background CD19-targeted chimeric antigen receptor T (CAR-T) cell therapy stands out as a revolutionary intervention, exhibiting remarkable remission rates in patients with refractory/relapsed (R/R) B-cell malignancies. However, the potential side effects of therapy, particularly cytokine release syndrome (CRS) and infections, pose significant challenges due to their overlapping clinical features. Promptly distinguishing between CRS infection post CD19 target CAR-T infusion (CTI) remains dilemma. Our study aimed analyze incidence infections identify key indicators for early detection febrile within 30 days post-CTI Methods In this retrospective cohort study, 104 consecutive R/R malignancies who underwent was reviewed. Clinical data including age, gender, CRS, ICANS, treatment history, incidence, responses were collected. Serum biomarkers procalcitonin (PCT), interleukin-6 (IL-6), C-reactive protein (CRP) levels analyzed using chemiluminescent assays. Statistical analyses employed Pearson’s Chi-square test, t-test, Mann–Whitney U-test, Kaplan–Meier survival analysis, Cox proportional hazards regression model, Spearman rank correlation, receiver operating characteristic (ROC) curve analysis evaluate diagnostic accuracy develop predictive models through multivariate logistic regression. Results 38 (36.5%) experienced (30 bacterial, 5 fungal, 3 viral) first CAR T-cell infusion. general, viral detected at median 7, 8, 9 days, respectively, after Prior allogeneic hematopoietic transplantation (HCT) an independent risk factor (Hazard Ratio [HR]: 4.432 [1.262–15.565], P = 0.020). Furthermore, both ((HR: 2.903 [1.577–5.345], < 0.001) severe (9.040 [2.256–36.232], 0.001). PCT, IL-6, CRP valuable prediction post-CAR-T PCT highest area under ROC (AUC) 0.897. A model incorporating demonstrated AUC 0.903 sensitivity specificity above 83%. For severity showed exceptional 0.991 perfect high specificity. Based on aforementioned we proposed workflow rapid identification during therapy. Conclusions prior HCT are factors malignancy patients. novel predicting infection, offers guide therapeutic decisions enhance efficacy future.
Language: Английский
Citations
2
Enzyme and Microbial Technology, Journal Year: 2023, Volume and Issue: 174, P. 110380 - 110380
Published: Dec. 15, 2023
Language: Английский
Citations
5
Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12
Published: Oct. 21, 2024
Background Sepsis is a common disease associated with neonatal and infant mortality, for diagnosis, blood culture currently the gold standard method, but it has low positivity rate requires more than 2 days to develop. Meanwhile, unfortunately, specific biomarkers early timely diagnosis of sepsis in infants determination severity this are lacking clinical practice. Methods Samples from 18 comorbidities, 25 without noninfectious diseases were evaluated using serum metabolomics approach based on liquid chromatography‒mass spectrometry (LC‒MS) technology. Differentially abundant metabolites screened via multivariate statistical analysis. In addition, least absolute shrinkage selection operator (LASSO) support vector machine recursive feature elimination (SVM-RFE) analyses conducted identify key infections. The random forest algorithm was applied determine differentially between comorbidities. Receiver operating characteristic (ROC) curves generated biomarker value testing. Finally, metabolic pathway analysis explore signaling pathways identified metabolites. Results A total 189 exhibited significant differences infectious infants, while 137 distinct septic After screening LASSO SVM-RFE analyses, hexylamine, psychosine sulfate, LysoPC (18:1 (9Z)/0:0), 2,4,6-tribromophenol, 25-cinnamoyl-vulgaroside retained sepsis. ROC curve revealed that area under (AUC) 0.9200 0.9749 0.9684 0.7405 0.8893 25-cinnamoyl-vulgaroside, 1.000 combination all When comorbidities compared those four endogenous greatest importance algorithm, namely, 12-oxo-20-trihydroxy-leukotriene B4, dihydrovaltrate, PA (8:0/12:0), 2-heptanethiol. these AUC 1 Pathway indicated phenylalanine, tyrosine, tryptophan biosynthesis, phenylalanine metabolism, porphyrin metabolism play important roles Conclusion Serum metabolite profiles identified, learning diseases. findings obtained expected facilitate disease.
Language: Английский
Citations
1
Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 7777 - 7791
Published: Oct. 1, 2024
Identifying high-risk children with poor prognoses during the early stages of sepsis and providing timely appropriate interventions are imperative. The objective this study was to develop a prognostic prediction model for pediatric utilizing neutrophil lymphocyte platelet ratio (NLPR).
Language: Английский
Citations
1
Global Pediatrics, Journal Year: 2024, Volume and Issue: 9, P. 100189 - 100189
Published: June 3, 2024
Neonatal sepsis is a frequent disease affecting newborns worldwide. It can be life threatening causing multi-organ dysfunction. We present case of 28-day old neonate who was admitted for an acute kidney injury in the context neonatal sepsis. Management conservative and successful, consisted adequately treating infection with antibiotics, correcting electrolyte imbalances, judiciously administrating fluids.
Language: Английский
Citations
0