Investigating SARS-CoV-2 Neutralising Antibody Response in Sheep DOI Creative Commons
Milena Samojlović, João R. Mesquita, Sérgio Santos‐Silva

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 13(1), P. 49 - 49

Published: Dec. 30, 2024

SARS-CoV-2 can cause clinical and inapparent disease mortality in several animals cohabitating with humans, sheep are susceptible to due virus–receptor interactions similar those humans. Hence, have the potential be infected, spread, develop neutralising antibodies (NAbs) against SARS-CoV-2. The aim of this study was investigate prevalence NAbs farm after natural exposure virus. Serum samples were collected from Serra da Estrela region Portugal, both prior during COVID-19 pandemic. sera tested by established pseudovirus systems for multiple variants (early—Wuhan, mid—Delta, Omicron—BA.1, late—Omicron XBB, BQ.1.1). Partial neutralisation activity Pre-pandemic Mid-pandemic observed, while no NAb observed Late-pandemic tested. Different levels between periods (p ≤ 0.01). Our results indicate that cross-species transmission may occurred through human–sheep contacts on farms pandemic, could contribute One Health Approach zoonotic virus surveillance pandemic preparedness.

Language: Английский

Animal models for COVID-19: advances, gaps and perspectives DOI Creative Commons
Changfa Fan, Yong Wu,

Rui Xiong

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: July 7, 2022

Abstract COVID-19, caused by SARS-CoV-2, is the most consequential pandemic of this century. Since outbreak in late 2019, animal models have been playing crucial roles aiding rapid development vaccines/drugs for prevention and therapy, as well understanding pathogenesis SARS-CoV-2 infection immune responses hosts. However, current some deficits there an urgent need novel to evaluate virulence variants concerns (VOC), antibody-dependent enhancement (ADE), various comorbidities COVID-19. This review summarizes clinical features COVID-19 different populations, characteristics major including those naturally susceptible animals, such non-human primates, Syrian hamster, ferret, minks, poultry, livestock, mouse sensitized genetically modified, AAV/adenoviral transduced, mouse-adapted strain engraftment human tissues or cells. host receptors proteases essential designing advanced modified models, successful studies on are also reviewed. Several improved alternatives future proposed, reselection alternative receptor genes multiple gene combinations, use transgenic knock-in method, strains establishing next generation mice.

Language: Английский

Citations

84
Competition of SARS-CoV-2 variants on the pandemic transmission dynamics DOI Open Access
Jiaqi Chen,

Changgui Gu,

Zhongyuan Ruan

et al.

Chaos Solitons & Fractals, Journal Year: 2023, Volume and Issue: 169, P. 113193 - 113193

Published: Feb. 10, 2023

Language: Английский

Citations

17
Mouse-Adapted SARS-CoV-2 MA10 Strain Displays Differential Pulmonary Tropism and Accelerated Viral Replication, Neurodissemination, and Pulmonary Host Responses in K18-hACE2 Mice DOI Creative Commons
Côme Thieulent,

Wellesley Dittmar,

Udeni B. R. Balasuriya

et al.

mSphere, Journal Year: 2023, Volume and Issue: 8(1)

Published: Feb. 2, 2023

The COVID-19 pandemic, caused by SARS-CoV-2, is still significantly impacting health care systems around the globe. Refined animal models are needed to study SARS-CoV-2 pathogenicity as well efficacy of vaccines and therapeutics.

Language: Английский

Citations

16
ACE2 and TMPRSS2 distribution in the respiratory tract of different animal species and its correlation with SARS-CoV-2 tissue tropism DOI Creative Commons
Mariano Carossino, Sudeh Izadmehr, Jessie D. Trujillo

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(2)

Published: Jan. 17, 2024

A wide range of animal species show variable susceptibility to SARS-CoV-2; however, host factors associated with varied remain be defined. Here, we examined whether SARS-CoV-2 and virus tropism in different are dependent on the expression distribution receptor angiotensin-converting enzyme 2 (

Language: Английский

Citations

6
Antibodies against SARS-CoV-2 Suggestive of Single Events of Spillover to Cattle, Germany DOI Creative Commons
Kerstin Wernike, Jens Böttcher,

Silke Amelung

et al.

Emerging infectious diseases, Journal Year: 2022, Volume and Issue: 28(9), P. 1916 - 1918

Published: Aug. 5, 2022

Human infection with SARS-CoV-2 poses a risk for transmission to animals. To characterize the cattle, we serologically investigated 1,000 samples collected from cattle in Germany late 2021. Eleven antibody-positive indicated that may be occasionally infected by contact SARS-CoV-2-positive keepers, but found no indication of further spread.

Language: Английский

Citations

22
Characterization of Entry Pathways, Species-Specific Angiotensin-Converting Enzyme 2 Residues Determining Entry, and Antibody Neutralization Evasion of Omicron BA.1, BA.1.1, BA.2, and BA.3 Variants DOI
Sabari Nath Neerukonda, Richard Wang, Russell Vassell

et al.

Journal of Virology, Journal Year: 2022, Volume and Issue: 96(17)

Published: Aug. 24, 2022

The SARS-CoV-2 Omicron variants were first detected in November 2021, and several lineages (BA.1, BA.2, BA.3, BA.4, BA.5) have since rapidly emerged. Studies characterizing the mechanisms of variant infection sensitivity to neutralizing antibodies induced upon vaccination are ongoing by groups. In present study, we used pseudoviruses show that transmembrane serine protease 2 (TMPRSS2) enhances BA.1, BA.1.1, BA.3 a lesser extent than ancestral D614G. We further higher inhibition soluble angiotensin-converting enzyme (ACE2) endosomal inhibitor chloroquine compared also more efficiently ACE2 receptors from 9 out 10 animal species tested, unlike D614G variant, mouse due Q493R Q498R spike substitutions. Finally, neutralization three doses Pfizer/BNT162b2 mRNA vaccine was 7- 8-fold less potent These results provide insights into transmissibility immune evasion capacity emerging curb their spread. IMPORTANCE emergence with an extensive number mutations poses significant public health zoonotic concern enhanced transmission fitness escape antibodies. studied lineage BA.3) found has influence on entry cells D614G, exhibits greater addition, displays efficient usage diverse Furthermore, Q493R/Q498R substitutions spike, Omicron, but not can use receptor. elicit high titers against variants, although still lower those may give

Language: Английский

Citations

17
Detection of SARS-CoV-2 in Terrestrial Animals in Southern Nigeria: Potential Cases of Reverse Zoonosis DOI Creative Commons
Anise N. Happi, Akeemat Opeyemi Ayinla, Olusola Ogunsanya

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(5), P. 1187 - 1187

Published: May 17, 2023

Since SARS-CoV-2 caused the COVID-19 pandemic, records have suggested occurrence of reverse zoonosis pets and farm animals in contact with SARS-CoV-2-positive humans Occident. However, there is little information on spread virus among Africa. Therefore, this study aimed to investigate various Nigeria. Overall, 791 from Ebonyi, Ogun, Ondo, Oyo States, Nigeria were screened for using RT-qPCR (n = 364) IgG ELISA 654). positivity rates 45.9% (RT-qPCR) 1.4% (ELISA). RNA was detected almost all animal taxa sampling locations except State. IgGs only goats Ebonyi pigs Ogun States. infectivity higher 2021 than 2022. Our highlights ability infect animals. It presents first report natural infection poultry, pigs, domestic ruminants, lizards. The close human–animal interactions these settings suggest ongoing zoonosis, highlighting role behavioral factors transmission potential These underscore importance continuous monitoring detect intervene any eventual upsurge.

Language: Английский

Citations

10
Retrospective case study of the impacts of multiple One Health oriented biocontainment research facilities during the SARS-CoV-2 pandemic DOI Creative Commons

Renata S.M. Landers,

Paul E. Hodgson,

Michael Puckette

et al.

One Health, Journal Year: 2025, Volume and Issue: unknown, P. 101061 - 101061

Published: May 1, 2025

Language: Английский

Citations

0
Transmission of severe acute respiratory syndrome coronavirus 2 from humans to animals: is there a risk of novel reservoirs? DOI Creative Commons
Leira Fernández‐Bastit, Júlia Vergara‐Alert, Joaquím Segalés

et al.

Current Opinion in Virology, Journal Year: 2023, Volume and Issue: 63, P. 101365 - 101365

Published: Oct. 2, 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a zoonotic virus able to infect humans and multiple nonhuman animal species. Most natural infections in companion, captive zoo, livestock, wildlife species have been related reverse transmission, raising concern about potential generation of reservoirs due human–animal interactions. To date, American mink white-tailed deer are the only that led extensive intraspecies transmission SARS-CoV-2 after zoonosis, leading an efficient spread subsequent animal-to-human transmission. Viral host adaptations increase probability new variants' emergence could cause major global health impact. Therefore, applying One Health approach crucial prevent overcome future threats for human, animal, environmental fields.

Language: Английский

Citations

9
Development and optimization of sampling techniques for environmental samples from African swine fever virus-contaminated surfaces with no organic contaminants DOI Creative Commons
Taeyong Kwon, Jordan T Gebhardt, Eu Lim Lyoo

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: July 18, 2024

African swine fever (ASF) is a highly contagious diseases in domestic pigs and wild boars with up to 100% mortality. ASF virus (ASFV) causative agent responsible for resistant environments, which creates significant challenge the control eradication of virus. Despite geographical expansion ASFV international movement products sustain production system, there limited knowledge on use environmental samples perform surveillance prevent introduction into ASFV-free areas transmission affected areas. Therefore, this study aimed develop optimize sampling techniques detection. The stainless steel surfaces were contaminated ASFV-infected blood, swabbed using different devices, then processed through techniques. tested qPCR analysis. results showed that pre-moistened gauze surgical sponges, sweeping pads, sponge sticks resulted increased sensitivity, when compared either dry devices or Dacron swab. In particular, combination stick commercial nucleic acid preservative supported best detection DNA clean evaluated. Pre-incubation short period time centrifugation at low speed sufficient provide satisfactory diagnostic sensitivity samples. Our findings contribute development dissemination ASFV.

Language: Английский

Citations

3