The biochemistry and function of pannexin channels

Biochimica et Biophysica Acta (BBA) - Biomembranes, Journal Year: 2012, Volume and Issue: 1828(1), P. 15 - 22

Published: Jan. 31, 2012

Language: Английский

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Bone physiology as inspiration for tissue regenerative therapies

Biomaterials, Journal Year: 2018, Volume and Issue: 185, P. 240 - 275

Published: Sept. 17, 2018

Language: Английский

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Intercellular Ca²⁺Waves: Mechanisms and Function

Physiological Reviews, Journal Year: 2012, Volume and Issue: 92(3), P. 1359 - 1392

Published: July 1, 2012

Intercellular calcium (Ca 2+ ) waves (ICWs) represent the propagation of increases in intracellular Ca through a syncytium cells and appear to be fundamental mechanism for coordinating multicellular responses. ICWs occur wide diversity have been extensively studied vitro. More recent studies focus on vivo. are triggered by variety stimuli involve release from internal stores. The predominately involves cell communication with messengers moving via gap junctions or extracellular mediating paracrine signaling. important both normal physiology as well pathophysiological processes organs tissues including brain, liver, retina, cochlea, vascular tissue. We review here mechanisms initiation ICWs, key intra- (inositol 1,4,5-trisphosphate ATP) proposed physiological functions ICWs.

Language: Английский

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Vesicular and conductive mechanisms of nucleotide release

Purinergic Signalling, Journal Year: 2012, Volume and Issue: 8(3), P. 359 - 373

Published: April 12, 2012

Language: Английский

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Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-ß-catenin

eLife, Journal Year: 2020, Volume and Issue: 9

Published: March 18, 2020

Mechanical forces are fundamental regulators of cell behaviors. However, molecular regulation mechanotransduction remain poorly understood. Here, we identified the mechanosensitive channels Piezo1 and Piezo2 as key force sensors required for bone development osteoblast differentiation. Loss Piezo1, or more severely Piezo1/2, in mesenchymal progenitor cells, led to multiple spontaneous fractures newborn mice due inhibition differentiation increased resorption. In addition, loss Piezo1/2 rendered resistant further caused by unloading both homeostasis. Mechanistically, relayed fluid shear stress extracellular matrix stiffness signals activate Ca2+ influx stimulate Calcineurin, which promotes concerted activation NFATc1, YAP1 ß-catenin transcription factors inducing their dephosphorylation well NFAT/YAP1/ß-catenin complex formation. Yap1 activities were reduced mutant bones such defects partially rescued enhanced activities.

Language: Английский

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ATP release through pannexon channels

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2015, Volume and Issue: 370(1672), P. 20140191 - 20140191

Published: May 26, 2015

Extracellular adenosine triphosphate (ATP) serves as a signal for diverse physiological functions, including spread of calcium waves between astrocytes, control vascular oxygen supply and ciliary beat in the airways. ATP can be released from cells by various mechanisms. This review focuses on channel-mediated release its main enabler, Pannexin1 (Panx1). Six subunits Panx1 form plasma membrane channel termed ‘pannexon’. Depending mode stimulation, pannexon has large conductance (500 pS) unselective permeability to molecules less than 1.5 kD or is small (50 pS), chloride-selective channel. Most pathological stimuli induce conformation, whereas conformation so far only been observed with exclusive voltage activation The interaction pannexons intimate. not conduit ATP, permitting efflux down concentration gradient, but also modulated ATP. activated through both ionotropic P2X well metabotropic P2Y purinergic receptors. In absence mechanism, this positive feedback loop would lead cell death owing linkage receptors apoptotic processes. A mechanism preventing excessive provided binding (with low affinity) protein gating shut.

Language: Английский

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ATP Release Channels

International Journal of Molecular Sciences, Journal Year: 2018, Volume and Issue: 19(3), P. 808 - 808

Published: March 11, 2018

Adenosine triphosphate (ATP) has been well established as an important extracellular ligand of autocrine signaling, intercellular communication, and neurotransmission with numerous physiological pathophysiological roles. In addition to the classical exocytosis, non-vesicular mechanisms cellular ATP release have demonstrated in many cell types. Although large negatively charged molecules cannot diffuse across lipid bilayer plasma membrane, conductive from cytosol into space is possible through ATP-permeable channels. Such channels must possess two minimum qualifications for permeation: anion permeability a ion-conducting pore. Currently, five groups are acknowledged ATP-release channels: connexin hemichannels, pannexin 1, calcium homeostasis modulator 1 (CALHM1), volume-regulated (VRACs, also known volume-sensitive outwardly rectifying (VSOR) channels), maxi-anion (MACs). Recently, major breakthroughs made field by molecular identification CALHM1 action potential-dependent channel taste bud cells, LRRC8s components VRACs, SLCO2A1 core subunit MACs. Here, function roles these summarized, along discussion on future implications understanding

Language: Английский

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Cell signaling and transcriptional regulation of osteoblast lineage commitment, differentiation, bone formation, and homeostasis

Cell Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: July 2, 2024

Abstract The initiation of osteogenesis primarily occurs as mesenchymal stem cells undergo differentiation into osteoblasts. This process plays a crucial role in bone formation and homeostasis is regulated by two intricate processes: cell signal transduction transcriptional gene expression. Various essential signaling pathways, including Wnt, BMP, TGF-β, Hedgehog, PTH, FGF, Ephrin, Notch, Hippo, Piezo1/2, play critical facilitating osteoblast differentiation, formation, homeostasis. Key factors this include Runx2, Cbfβ, Runx1, Osterix, ATF4, SATB2, TAZ/YAP. Furthermore, diverse array epigenetic also roles at the level. review provides an overview latest developments current comprehension concerning pathways signaling, regulation hormones, genes involved commitment lineage, well maintenance paper reviews via mechanisms, such histone DNA modifications. Additionally, we summarize biology spurred recent advancements various modern technologies bioinformatics. By synthesizing these insights comprehensive understanding further clarification mechanisms underlying lineage commitment, highlights potential new therapeutic applications for treatment diseases.

Language: Английский

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Biological role of connexin intercellular channels and hemichannels

Archives of Biochemistry and Biophysics, Journal Year: 2012, Volume and Issue: 524(1), P. 2 - 15

Published: March 17, 2012

Language: Английский

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Gap-junction-mediated cell-to-cell communication

Cell and Tissue Research, Journal Year: 2012, Volume and Issue: 352(1), P. 21 - 31

Published: Aug. 31, 2012

Language: Английский

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