ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance DOI Creative Commons
Eunice N. Kim,

Feng-Qian Li,

Ken‐Ichi Takemaru

et al.

Life Science Alliance, Journal Year: 2024, Volume and Issue: 8(2), P. e202402916 - e202402916

Published: Dec. 2, 2024

Bin/Amphiphysin/Rvs (BAR) domains are highly conserved found in all eukaryotes. BAR domain proteins form crescent-shaped dimers that sense and sculpt curved lipid membranes play key roles various cellular processes. However, their functions mammalian development poorly understood. We previously demonstrated Chibby1-interacting domain–containing 1 (ciBAR1, formerly known as FAM92A) localizes to the ciliary base plays a critical role ciliogenesis. Here, we report ciliopathy phenotypes of ciBAR1 -KO mice. ∼28% mice show embryonic lethality because randomized left–right asymmetry; rest survive into adulthood with no gross morphological abnormalities. Histological assessments ciliated tissues revealed exocrine pancreatic lesions. Although overall endocrine islet morphology appeared be normal, showed impaired glucose tolerance. Examination ductal cilia number length were significantly reduced pancreata. MEFs also exhibited defects. Our findings indicate ciliogenesis depending on tissue cell type

Language: Английский

Development of functional spermatozoa in mammalian spermiogenesis DOI
Haruhiko Miyata, Keisuke Shimada, Yuki Kaneda

et al.

Development, Journal Year: 2024, Volume and Issue: 151(14)

Published: July 15, 2024

ABSTRACT Infertility is a global health problem affecting one in six couples, with 50% of cases attributed to male infertility. Spermatozoa are gametes, specialized cells that can be divided into two parts: the head and flagellum. The contains vesicle called acrosome undergoes exocytosis flagellum motility apparatus propels spermatozoa forward components, axonemes accessory structures. For fertilize oocytes, must formed correctly. In this Review, we describe comprehensively how functional develop mammals during spermiogenesis, including formation acrosomes, structures by focusing on analyses mouse models.

Language: Английский

Citations

10

The annulus: composition, role and importance in sperm flagellum biogenesis and male fertility DOI Creative Commons
Marjorie Whitfield

Basic and Clinical Andrology, Journal Year: 2024, Volume and Issue: 34(1)

Published: Dec. 15, 2024

Abstract The annulus is an electron-dense ring structure that surrounds the axoneme and compartmentalizes sperm flagellum into two parts: midpiece principal piece. function of as a diffusion barrier in mature spermatozoon now well described but its during spermiogenesis remains unclear. intriguing spatio-temporal dynamics position at interface main flagellar compartments have been highlighted for more than 50 years, suggest major role this process. During last decade, numerous studies contributed establishing repertoire proteins known to be located annulus. Mutant mouse models invalidation these provided essential information clues novel hypotheses regarding functions regulation structure. Importantly, recent identification humans homozygous mutations genes coding leading sterility reinforced importance physiology male fertility. This review provides comprehensive description all knowledge obtained several years composition functions, both mice humans.

Language: Английский

Citations

1

Centriolar appendages evolve into the inner sheath of mammalian flagella DOI Creative Commons
J. Chen, Mingxi Liu

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 223(3)

Published: Feb. 21, 2024

The annulus, a septin-based structure in vertebrate sperm connecting the MP and PP, has unclear migration mechanics. In this issue, Hoque et al. (https://doi.org/10.1083/jcb.202307147) report that CBY3/CIBAR1 complex ensures its precise positioning by regulating membrane properties.

Language: Английский

Citations

0

ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance DOI Creative Commons
Eunice N. Kim,

Feng-Qian Li,

Ken‐Ichi Takemaru

et al.

Life Science Alliance, Journal Year: 2024, Volume and Issue: 8(2), P. e202402916 - e202402916

Published: Dec. 2, 2024

Bin/Amphiphysin/Rvs (BAR) domains are highly conserved found in all eukaryotes. BAR domain proteins form crescent-shaped dimers that sense and sculpt curved lipid membranes play key roles various cellular processes. However, their functions mammalian development poorly understood. We previously demonstrated Chibby1-interacting domain–containing 1 (ciBAR1, formerly known as FAM92A) localizes to the ciliary base plays a critical role ciliogenesis. Here, we report ciliopathy phenotypes of ciBAR1 -KO mice. ∼28% mice show embryonic lethality because randomized left–right asymmetry; rest survive into adulthood with no gross morphological abnormalities. Histological assessments ciliated tissues revealed exocrine pancreatic lesions. Although overall endocrine islet morphology appeared be normal, showed impaired glucose tolerance. Examination ductal cilia number length were significantly reduced pancreata. MEFs also exhibited defects. Our findings indicate ciliogenesis depending on tissue cell type

Language: Английский

Citations

0