COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas

Cell, Journal Year: 2021, Volume and Issue: 184(7), P. 1895 - 1913.e19

Published: Feb. 5, 2021

Language: Английский

---

Effect of Hydrocortisone on 21-Day Mortality or Respiratory Support Among Critically Ill Patients With COVID-19

JAMA, Journal Year: 2020, Volume and Issue: 324(13), P. 1298 - 1298

Published: Sept. 2, 2020

Coronavirus disease 2019 (COVID-19) is associated with severe lung damage. Corticosteroids are a possible therapeutic option.

Language: Английский

---

A blood atlas of COVID-19 defines hallmarks of disease severity and specificity

Cell, Journal Year: 2022, Volume and Issue: 185(5), P. 916 - 938.e58

Published: Jan. 21, 2022

Treatment of severe COVID-19 is currently limited by clinical heterogeneity and incomplete description specific immune biomarkers. We present here a comprehensive multi-omic blood atlas for patients with varying severity in an integrated comparison influenza sepsis versus healthy volunteers. identify signatures correlates host response. Hallmarks disease involved cells, their inflammatory mediators networks, including progenitor cells myeloid lymphocyte subsets, features the repertoire, acute phase response, metabolism, coagulation. Persisting activation involving AP-1/p38MAPK was feature COVID-19. The plasma proteome enabled sub-phenotyping into patient clusters, predictive outcome. Systems-based integrative analyses tensor matrix decomposition all modalities revealed groupings linked specificity compared to sepsis. Our approach will support future drug development, trial design, personalized medicine approaches

Language: Английский

---

The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing

Cell Discovery, Journal Year: 2020, Volume and Issue: 6(1)

Published: Oct. 20, 2020

Abstract Understanding the mechanism that leads to immune dysfunction in severe coronavirus disease 2019 (COVID-19) is crucial for development of effective treatment. Here, using single-cell RNA sequencing, we characterized peripheral blood mononuclear cells (PBMCs) from uninfected controls and COVID-19 patients paired broncho-alveolar lavage fluid (BALF). We found a close association decreased dendritic (DCs) increased monocytes resembling myeloid-derived suppressor (MDSCs), which correlated with lymphopenia inflammation patients. Those MDSC-like were immune-paralyzed. In contrast, monocyte-macrophages BALFs produced massive amounts cytokines chemokines, but secreted little interferons. The frequencies T NK significantly patients, especially innate-like various CD8 + cell subsets, compared healthy controls. proportions activated CD4 subsets among compartment, including Th1, Th2, Th17-like more clonally expanded Patients’ showed no sign exhaustion or augmented death, whereas higher levels IFNG , TNF CCL4 CCL5 etc. Paired TCR tracking indicated abundant recruitment patients’ lung. Together, this study comprehensively depicts how landscape perturbed COVID-19.

Language: Английский

---

Outcome of SARS-CoV-2 infection is linked to MAIT cell activation and cytotoxicity

Nature Immunology, Journal Year: 2021, Volume and Issue: 22(3), P. 322 - 335

Published: Feb. 2, 2021

Immune system dysfunction is paramount in coronavirus disease 2019 (COVID-19) severity and fatality rate. Mucosal-associated invariant T (MAIT) cells are innate-like involved mucosal immunity protection against viral infections. Here, we studied the immune cell landscape, with emphasis on MAIT cells, cohorts totaling 208 patients various stages of disease. frequency strongly reduced blood. They display a strong activated cytotoxic phenotype that more pronounced lungs. Blood alterations positively correlate activation other innate proinflammatory cytokines, notably interleukin (IL)-18, mortality severe acute respiratory syndrome 2 infection. We also identified monocyte/macrophage interferon (IFN)-α–IL-18 cytokine shift ability infected macrophages to induce cytotoxicity an MR1-dependent manner. Together, our results suggest altered functions due IFN-α–IL-18 imbalance contribute severity, their therapeutic manipulation may prevent deleterious inflammation COVID-19 aggravation. Severe characterized by hyperinflammation, there need for accurate predictive biomarkers progression. Lehuen et al. demonstrate show dramatic loss those do remain highly state.

Language: Английский

---

MAIT cell activation and dynamics associated with COVID-19 disease severity

Science Immunology, Journal Year: 2020, Volume and Issue: 5(51)

Published: Sept. 18, 2020

MAIT cell activation and decline in blood are associated with COVID-19 severity, features that dynamically recover convalescence.

Language: Английский

---

Mucosal immune responses to infection and vaccination in the respiratory tract

Immunity, Journal Year: 2022, Volume and Issue: 55(5), P. 749 - 780

Published: May 1, 2022

Language: Английский

---

The impact of pre-existing cross-reactive immunity on SARS-CoV-2 infection and vaccine responses

Nature reviews. Immunology, Journal Year: 2022, Volume and Issue: 23(5), P. 304 - 316

Published: Dec. 20, 2022

Language: Английский

---

γδ T cells: origin and fate, subsets, diseases and immunotherapy

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: Nov. 22, 2023

The intricacy of diseases, shaped by intrinsic processes like immune system exhaustion and hyperactivation, highlights the potential renormalization as a promising strategy in disease treatment. In recent years, our primary focus has centered on γδ T cell-based immunotherapy, particularly pioneering use allogeneic Vδ2

Language: Английский

---

T cell immunity to SARS-CoV-2 following natural infection and vaccination

Biochemical and Biophysical Research Communications, Journal Year: 2020, Volume and Issue: 538, P. 211 - 217

Published: Oct. 23, 2020

Language: Английский

---