On the link between antibiotic resistance, diabetes, and wastewater DOI Open Access
Shimon Schuldiner

The Journal of General Physiology, Journal Year: 2024, Volume and Issue: 156(3)

Published: Jan. 31, 2024

The study by Lucero et al. (https://doi.org/10.1085/jgp.202313464) sheds light on the remarkable capabilities of bacterial transporters to adapt new selective pressures. Their findings provide insight into mechanism a subtype SMR transporters.

Language: Английский

Conserved marseilleviruses harboring diverse antibiotic resistance genes isolated from the Yangtze river Delta and the Pearl river delta, China DOI Creative Commons
Yucheng Xia, Baiyu Su,

Hongwei Ren

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 27, 2025

Marseilleviruses are a group of double-stranded DNA viruses infecting Acanthamoeba within the phylum Nucleocytoviricota and ubiquitous in water soil globally. Here, we report six strains marseilleviruses isolated from environmental samples Yangtze River Delta Pearl Delta, China. Viral particles exhibited icosahedral shaped capsids measuring about 220 ~ 240 nm diameter. Based on stability assays, viral were halotolerant acid-tolerant, but sensitive to chloroform high temperature. Genomics phylogenetic analyses showed that these highly conserved compared with other reported marseilleviruses. Diverse members small multidrug resistance (SMR) family transporter, which is type antibiotics gene (ARG) contribute feature antibiotic bacteria, our best knowledge, firstly described Marseilleviridae. The alignments primary structures in-silico tertiary reveal structural potential functional similarity between giant bacterial SMR, suggesting possible role viruses' interaction antibiotics. biological properties marseillevirus discovery SMR provide insight external intracellular environment fitness large amoeba-infecting viruses.

Language: Английский

Citations

0
SMR transporters meet the challenge of metformin metabolites DOI Creative Commons
Ben Short

The Journal of General Physiology, Journal Year: 2024, Volume and Issue: 156(3)

Published: Feb. 7, 2024

JGP study (Lucero et al. http://www.doi.org/10.1085/jgp.202313464) shows that members of the SMRGdx subtype can export degradation products metformin, helping bacteria adapt to high environmental levels commonly prescribed diabetes medication.

Language: Английский

Citations

1
Peripheral mutations underlie promiscuous transport of quaternary ammonium antiseptics by Small Multidrug Resistance transporters DOI Creative Commons
Olive E. Burata,

Ever O’Donnell,

Jeonghoon Hyun

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 7, 2024

Abstract The mechanistic basis of transport promiscuity in multidrug exporters is not well understood. We examine this question using the Small Multidrug Resistance (SMR) transporters. engineer a selective SMR protein to promiscuously export quaternary ammonium antiseptics, similar family. Using combinatorial mutagenesis and deep sequencing, we identify necessary sufficient molecular determinants new activity. x-ray crystallography, electrophysiology, novel proteoliposome-based antiseptic assay, tease apart roles that these residues play polyspecificity. find substrate preference changes through modification directly interact with substrate, but mutations peripheral binding pocket. Our insights into among SMRs can be applied understand evolution functions more generally.

Language: Английский

Citations

1
Peripheral positions encode transport specificity in the small multidrug resistance exporters DOI Creative Commons
Olive E. Burata,

Ever O’Donnell,

Jeonghoon Hyun

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(25)

Published: June 12, 2024

In secondary active transporters, a relatively limited set of protein folds have evolved diverse solute transport functions. Because the conformational changes inherent to transport, altering substrate specificity typically involves remodeling entire structural landscape, limiting our understanding how novel specificities evolve. current work, we examine structurally minimalist family model proteins, small multidrug resistance (SMR) understand molecular basis for emergence specificity. We engineer selective SMR promiscuously export quaternary ammonium antiseptics, similar activity clade exporters in this family. Using combinatorial mutagenesis and deep sequencing, identify necessary sufficient determinants engineered activity. X-ray crystallography, solid-supported membrane electrophysiology, binding assays, proteoliposome-based antiseptic assay that developed, dissect mechanistic contributions these residues polyspecificity. find preference not through modification directly interact with but mutations peripheral pocket. Our work provides insight into promiscuity among SMRs can be applied evolution functions more generally.

Language: Английский

Citations

0
On the link between antibiotic resistance, diabetes, and wastewater DOI Open Access
Shimon Schuldiner

The Journal of General Physiology, Journal Year: 2024, Volume and Issue: 156(3)

Published: Jan. 31, 2024

The study by Lucero et al. (https://doi.org/10.1085/jgp.202313464) sheds light on the remarkable capabilities of bacterial transporters to adapt new selective pressures. Their findings provide insight into mechanism a subtype SMR transporters.

Language: Английский

Citations

0