Bioprinting of Cells, Organoids and Organs-on-a-Chip Together with Hydrogels Improves Structural and Mechanical Cues

Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1638 - 1638

Published: Oct. 1, 2024

The 3D bioprinting technique has made enormous progress in tissue engineering, regenerative medicine and research into diseases such as cancer. Apart from individual cells, a collection of organoids, can be printed combination with various hydrogels. It hypothesized that will even become promising tool for mechanobiological analyses organoids their matrix environments highly defined precisely structured environments, which the mechanical properties cell environment individually adjusted. Mechanical obstacles or bead markers integrated bioprinted samples to analyze deformations forces within these constructs, perform biophysical analysis complex systems, are still not standard techniques. review highlights advances 4D printing technologies integrating cues so next step detailed key future directions organoid generation development disease model regeneration drug testing perspective. Finally, hydrogels, pure natural synthetic hydrogels mixtures, organoid–cell co-cultures, organ-on-a-chip systems organoid-organ-on-a chip combinations introduces use assembloids determine mutual interactions different types cell–matrix interferences specific biological environments.

Language: Английский

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Review of New Approach Methodologies for Application in Risk Assessment of Nanoparticles in the Food and Feed Sector: Status and Challenges

EFSA Supporting Publications, Journal Year: 2024, Volume and Issue: 21(9)

Published: Sept. 1, 2024

Abstract New Approach Methodologies (NAMs), broadly understood to include in silico, chemico, vitro and ex vivo methods, show great potential advancing risk assessment albeit their regulatory implementation is lagging. The EFSA Guidance on of nanomaterials (EFSA Nano‐RA) suggests nano‐specific best achieved through Integrated Approaches Testing Assessment (IATAs) with NAMs as the first choice generate new information. Integrating promises several advantages such a better human focus, more detailed insights into molecular mechanisms higher efficacy. However, applying NMs also poses considerable challenges issues related dispersion stability, dosimetry, agglomeration, dissolution, transformations or assay interferences. Significant efforts are being undertaken by standardisation organisations research projects establish various for NMs. Here thorough review provided covering that will be potentially useful food feed sector. It follows structure Nano‐RA expands it, where needed, support decision‐making selection NM assessment. begins an overview NAM‐frameworks, followed description individual including those relevant physicochemical characterisation, exposure hazard toxicodynamics toxicokinetics. focus concerning degradation/dissolution, genotoxicity, cytotoxicity, oxidative stress, (pro‐)inflammation, barrier integrity important endpoints initial screening according framework. As result, total 267 nano‐relevant NAMs, mostly “not validated” (with few notable exceptions), were included this review. Validation notwithstanding, could already prove reliable NMs, especially integrated approaches.

Language: Английский

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Bioprinting spatially guided functional 3D neural circuits with agarose-xanthan gum copolymer hydrogels

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123156 - 123156

Published: Jan. 1, 2025

Language: Английский

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Spheroid‐on‐a‐Chip Platforms for Tumor Microenvironment and Drug Development

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Abstract Despite significant advancements in oncology research and therapeutic interventions, cancer continues to be the leading cause of mortality worldwide. The key challenge addressing this pressing issue lies lack precision diagnosis a limited understanding nature how tumor microenvironment responds interventions. Research focusing on impact (TME) heterogeneity response drugs is crucial ensure efficient therapy. Conventional models exhibit constraints, including inability accurately imitate tumors’ complex 3D architecture dynamic microenvironment. Recent developments Spheroid‐On‐a‐Chip (SoC) technology provide alternative, offering closer that human tissue. This review explores recent SoC modeling, highlighting spheroid formation mechanisms techniques. applications platform mimic essential features their potential role anticancer drug development are summarized. advantages challenges technologies behind devices compared traditional vitro further discussed. Lastly, future directions for transforming improving suggested.

Language: Английский

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3D bioprinting for bile duct tissue engineering: current status and prospects

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 14, 2025

Bile duct disorders, including cholangiocarcinoma, primary sclerosing cholangitis, and iatrogenic injuries, pose significant clinical challenges due to limited regenerative capacity the complexity of biliary tree. In recent years, 3D bioprinting has emerged as a promising approach for bile tissue engineering by providing patient-specific geometries facilitating spatial organization cells, scaffolding materials, bioactive factors. This review presents comprehensive overview techniques engineering, focusing on fundamental principles, biomaterial selection, current achievements, key challenges, future perspectives. We systematically discuss latest technological breakthroughs, highlight emerging innovations such organoid-based strategies microfluidic-assisted printing, evaluate prospects translation. Finally, we outline main challenges—such biocompatibility vascularization, immunological barriers, standardization protocols, regulatory hurdles—and propose directions research, emphasizing multidisciplinary collaboration translational studies.

Language: Английский

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Indirect Measurement of Cell Loading in Hydrogel Bioinks During Handling and Preparation of 3d Models

Published: Jan. 1, 2025

Language: Английский

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Enhancing organoid technology with carbon-based nanomaterial biosensors: Advancements, challenges, and future directions

Advanced Drug Delivery Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 115592 - 115592

Published: May 1, 2025

Language: Английский

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Organoids: new frontiers in tumor immune microenvironment research

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 29, 2024

The tumor microenvironment (TME) contains cells that regulate medication response and cancer growth in a major way. Tumor immunology research has been rejuvenated treatment changed by immunotherapy, rapidly developing therapeutic approach. patterns of vivo the heterogeneity, complexity, individuality tumors produced from patients are not reflected traditional two-dimensional cell profiles. On other hand, an vitro three-dimensional (3D) model called organoid is gaining popularity. It can replicate physiological pathological properties original tissues . source immune organoids. TME characteristics be preserved while preserving variety cultivating epithelial with various stromal immunological components. In addition to having genetic physical similarities human diseases ability partially reconstruct complex structure tumors, these models now widely used fields including cancer, developmental biology, regenerative mechanisms, drug development, disease modeling, organ transplantation. This study reviews function organoids immunotherapy milieu. We also discuss current developments suggest translational uses immuno-oncology research, precision medicine.

Language: Английский

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Advanced tumor organoid bioprinting strategy for oncology research

Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101198 - 101198

Published: Aug. 9, 2024

Bioprinting is a groundbreaking technology that enables precise distribution of cell-containing bioinks to construct organoid models accurately reflect the characteristics tumors

Language: Английский

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Advances and Challenges of Bioassembly Strategies in Neurovascular In Vitro Modeling: An Overview of Current Technologies with a Focus on Three-Dimensional Bioprinting

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11000 - 11000

Published: Oct. 12, 2024

Bioassembly encompasses various techniques such as bioprinting, microfluidics, organoids, and self-assembly, enabling advances in tissue engineering regenerative medicine. Advancements bioassembly technologies have enabled the precise arrangement integration of cell types to more closely mimic complexity functionality neurovascular unit (NVU) that other biodiverse multicellular structures. In this context, bioprinting offers ability deposit cells a spatially controlled manner, facilitating construction interconnected networks. Scaffold-based assembly strategies provide structural support guidance cues for growth, formation complex bio-constructs. Self-assembly approaches utilize inherent properties drive spontaneous organization interaction neuronal vascular components. However, recreating intricate microarchitecture functional characteristics tissue/organ poses additional challenges. materials hold great promise addressing these The further refinement technologies, improved resolution incorporation multiple types, can enhance accuracy biological constructs; however, developing bioinks growth cells, viability, while maintaining compatibility with process remains an unmet need field, advancements design bioactive biodegradable scaffolds will aid controlling adhesion, differentiation, vascularization within engineered tissue. Additionally, integrating advanced imaging analytical real-time monitoring characterization bioassembly, aiding quality control optimization. While challenges remain, ongoing research technological propel field forward, paving way transformative developments engineering. This work provides overview advancements, challenges, future perspectives fabricating constructs add-on focus on technologies.

Language: Английский

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