Biology,
Journal Year:
2023,
Volume and Issue:
12(2), P. 323 - 323
Published: Feb. 16, 2023
Toll-like
receptors
(TLRs)
have
been
implicated
in
the
regulation
of
various
metabolism
pathways,
addition
to
their
function
innate
immunity.
Here,
we
investigate
metabolic
TLR2
a
larval
zebrafish
system.
We
studied
larvae
from
tlr2
mutant
and
wild
type
sibling
controls
an
unchallenged
normal
developmental
condition
using
transcriptomic
metabolomic
analyses
methods.
RNAseq
was
used
evaluate
differences
between
wild-type
control
found
signature
set
149
genes
be
significantly
altered
gene
expression.
The
expression
level
several
confirmed
by
qPCR
analyses.
Gene
enrichment
analysis
(GSEA)
revealed
differential
two
genotypes
related
valine,
leucine,
isoleucine
degradation
glycolysis
gluconeogenesis.
Using
1H
nuclear
magnetic
resonance
(NMR)
metabolomics,
that
glucose
metabolites
with
were
present
at
higher
levels
mutant.
Furthermore,
is
mutants
fluorometric
assay.
Therefore,
shown
TLR2,
its
immunity,
has
controlling
during
vertebrate
development.
functions
are
associated
transcriptional
enzymes
involved
could
explain
different
glucose,
lactate,
succinate,
malate
Frontiers in Environmental Science,
Journal Year:
2023,
Volume and Issue:
11
Published: Sept. 15, 2023
Fish
are
among
the
best-studied
aquatic
animals
due
to
their
economic
and
ecological
values.
meat
is
most
affordable
protein
source
for
economically
weaker
section
of
people.
The
environment
almost
all
ecosystems
has
a
specific
influential
role
on
or
by
fishes.
Therefore,
studying
stress
biology,
especially
oxidative
stress,
vital
because
it
can
influence
growth,
production,
reproduction,
etc.
To
review
above
topic,
peer-reviewed
electronic
databases,
including
Web
Science,
science
direct,
PubMed,
Google
Scholar,
Scopus,
AGRICOLA,
were
searched
with
keywords
associated
fish,
diseases,
abiotic
such
as
effects
water
dissolved
oxygen,
temperature,
salinity,
hardness,
alkalinity,
pH,
pollutants,
heavy
metals,
anthropogenic
activities,
was
reviewed
in
current
article
draw
conclusion
updated
relation
that
exists
between
fish
physiology,
disease,
stressors.
Oxidative
redox
regulatory
levels
under
parameters
anti-stress
responses
differ
various
models.
Undoubtedly,
factors
modulate
health
status
greater
extent,
therefore,
these
must
be
considered
priority
basis
improve
general
immunity
fish.
statement
remains
valid
both
saline
freshwater
habitats.
Journal of Toxicology and Environmental Health,
Journal Year:
2024,
Volume and Issue:
87(8), P. 357 - 370
Published: Feb. 2, 2024
Sodium
dodecylbenzene
sulfonate
(SDBS)
is
an
important
surfactant
used
as
a
cleaning
agent
and
industrial
additive
to
remove
unwanted
chemicals
which
have
been
detected
in
the
aquatic
environment.
The
aim
of
this
study
was
examine
toxicological
potential
SDBS
on
gills
adult
male
zebrafish
(Danio
rerio)
exposed
chemical.
For
96
hr
acute
exposure,
fish
were
divided
into
three
groups:
control,
0.25
mg/L,
0.5
mg/L
SDBS.
After
experiment,
morphophysiological
analyses
(gill
histopathology
histochemistry),
oxidative
stress
(determination
gill
activities
superoxide
dismutase
(SOD)
catalase
(CAT)),
hematological
(leukocyte
differentiation)
conducted.
Data
demonstrated
that
at
both
tested
concentrations
altered
histopathological
index
initiated
circulatory
disturbances,
well
adverse,
progressive,
immunological
changes
gills.
In
group,
SOD
activity
decreased
significantly,
but
CAT
not
altered.
Prominent
blood
observed
group
neutrophilia
lymphocytosis.
number
mucous
chloride
cells
increased
significantly
groups.
Taken
together,
our
findings
exposure
D.
rerio
SDBS,
even
for
hr,
produced
adverse
morphological
effects
associated
with
reduction
activity.
Our
indicate
species
anionic
may
lead
consequences
stress.
Therefore,
highlights
risks
substance
pose
ecosystems
emphasizes
need
further
investigations
strict
regulations
its
disposal.
PLoS ONE,
Journal Year:
2025,
Volume and Issue:
20(2), P. e0318212 - e0318212
Published: Feb. 21, 2025
Electron
paramagnetic
resonance
(EPR)
is
an
excellent
choice
for
detecting
free
radicals
in
biological
samples.
Biologically
relevant
are
extremely
short-lived
and
cannot
be
detected
directly,
emphasizing
the
need
appropriate
compound
to
generate
stable
adducts
that
can
measured
by
EPR.
Spin
trapping
with
nitrone
compounds
like
5,5-dimethyl-1-pyrroline
N-oxide
(DMPO)
a
method
commonly
employed
radicals.
However,
due
instability
of
radical
adducts,
using
cell-permeable
1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethyl
pyrrolidine
(CMH)
appears
more
effective
approach
within
tissues.
Here,
we
compare
use
DMPO
CMH
detect
most
abundant
reactive
oxygen
species
radical,
superoxide
(
O2⋅-
),
zebrafish
present
optimized
protocol
performing
EPR
spin
probe
both
hearts
larvae.
Together,
our
data
suggest
reliable
id="m2">
Toxicology International,
Journal Year:
2025,
Volume and Issue:
unknown, P. 57 - 63
Published: March 12, 2025
Water
parameters
are
essential
in
maintaining
the
good
health
of
aquatic
organisms.
Fluctuation
any
is
a
stress
factor
for
Among
them,
salinity
acts
as
significant
stress.
Zebrafish
have
substantial
responses
to
variations
salinity,
which
cause
changes
physiology
and
behaviour.
Fish
experiencing
fluctuations
content
their
surroundings
said
be
Excessive
can
dehydrate
freshwater
fish
lead
an
imbalance
ions;
however,
abrupt
reductions
saltwater
induce
influx
water
loss
ions.
exposed
prolonged
salt
more
sensitive
illness,
lower
general
fitness,
chance
surviving.
Major
Findings:
The
acute
toxicity
chronic
effects
extreme
levels
(6g/L,
7g/L,
8g/L,
9g/L,
10g/L)
evident
zebrafish
heart
tissue.
This
study
highlights
how
quality
parameters,
such
influence
specific
physiological
molecular
events
zebrafish.
In
summary,
exposure
sub-lethal
concentrations
significantly
impacts
physiology,
leading
increased
mortality.
