Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0

PLoS Biology, Journal Year: 2020, Volume and Issue: 18(7), P. e3000411 - e3000411

Published: July 14, 2020

Improving the reproducibility of biomedical research is a major challenge. Transparent and accurate reporting vital to this process; it allows readers assess reliability findings repeat or build upon work other researchers. The ARRIVE guidelines (Animal Research: Reporting In Vivo Experiments) were developed in 2010 help authors journals identify minimum information necessary report publications describing vivo experiments. Despite widespread endorsement by scientific community, impact on transparency animal has been limited. We have revised update them facilitate their use practice. are published alongside paper. This explanation elaboration document was as part revision. It provides further about each 21 items 2.0, including rationale supporting evidence for inclusion guidelines, details report, examples good from literature. also covers advice best practice design conduct studies support researchers improving standards start experimental process through publication.

Language: Английский

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Reporting animal research: Explanation and Elaboration for the ARRIVE guidelines 2019

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2019, Volume and Issue: unknown

Published: July 15, 2019

Abstract Improving the reproducibility of biomedical research is a major challenge. Transparent and accurate reporting are vital to this process; it allows readers assess reliability findings, repeat or build upon work other researchers. The NC3Rs developed ARRIVE guidelines in 2010 help authors journals identify minimum information necessary report publications describing vivo experiments. Despite widespread endorsement by scientific community, impact on transparency animal has been limited. We have revised update them facilitate their use practice. published alongside paper. This Explanation Elaboration document was as part revision. It provides further about each 21 items 2019, including rationale supporting evidence for inclusion guidelines, elaboration details report, examples good from literature.

Language: Английский

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Occasional paternal inheritance of the germline-restricted chromosome in songbirds

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(4)

Published: Jan. 20, 2022

Significance Most if not all songbirds possess a germline-restricted chromosome (GRC) which is believed to be exclusively maternally inherited. However, we show that, in the zebra finch, GRC can also paternally inherited and that potential for paternal inheritance may differ between families. We further genetic diversity of extremely reduced compared high mitochondrial DNA lineages, suggesting single haplotype has spread through Australian finch population relatively recently via occasional inheritance. Our study therefore suggests evolve selfish manner, could result intragenomic conflict.

Language: Английский

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Food Restriction During Development Impairs Reproductive Success but not Ornamentation in Zebra Finches

Journal of Experimental Zoology Part A Ecological and Integrative Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Stress during development can elicit lifelong changes to an organism. However, whether these are beneficial or detrimental only be determined by their effect on fitness outcomes. Furthermore, the of severe, chronic food restriction ornamentation, which influence attractiveness, still needs explored. In this study, zebra finches (Taeniopygia castanotis) were exposed either ad libitum (control) diet a 40% restricted (food-restricted) throughout postnatal development. Pictures beaks and cheek patches captured juvenile period into adulthood measure secondary sexual characteristics. adulthood, females paired with nonexperimental males reproductive success. There was no treatment beak color final color, but food-restricted developed slower had more asymmetry patch did not impact size. Food-restricted found have lower success than control females. These results suggest that while ornamentation experience 'catch-up growth', do reflect past developmental stress exposure, impairs female in short-lived passerine.

Language: Английский

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Studying estrildid finches to understand sexual and social signals in birds: a short review

acta ethologica, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Language: Английский

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Irreproducible text-book “knowledge”: The effects of color bands on zebra finch fitness

Evolution, Journal Year: 2018, Volume and Issue: 72(4), P. 961 - 976

Published: March 25, 2018

Many fields of science—including behavioral ecology—currently experience a heated debate about the extent to which publication bias against null findings results in misrepresentative scientific literature. Here, we show case an extreme mismatch between strong positive support for effect literature and failure detect this across multiple attempts at replication. For decades, researchers working with birds have individually marked their study species colored leg bands. zebra finch Taeniopygia guttata, model organism ecology, many studies over past 35 years reported effects bands certain colors on male or female attractiveness further behavior, physiology, life history, fitness. Only eight 39 publications presented exclusively findings. analyze experiments quantified fitness total 730 color‐banded individuals from four captive populations (two domesticated two recently wild derived). This sample size exceeds combined all 23 that clearly "color‐band effect" hypothesis. We found band color explains no variance either also heterogeneity color‐band effects, arguing both context population specificity. Analysis unpublished data three other laboratories strengthens generality our finding. Finally, meta‐analysis previously published is indicative selective reporting suggests approaches zero when large. argue field—and science general—would benefit more effective means counter confirmation bias.

Language: Английский

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Weak antagonistic fitness effects can maintain an inversion polymorphism

Molecular Ecology, Journal Year: 2023, Volume and Issue: 32(13), P. 3575 - 3585

Published: April 28, 2023

Abstract The study of chromosomal inversion polymorphisms has received much recent attention, particularly in cases where inversions have drastic effects on phenotypes and fitness (e.g. lethality homozygotes). Less attention been paid to the question maintenance that show only weak effects. Here, we such an polymorphism links 250 genes chromosome Tgu11 zebra finch ( Taeniopygia guttata ). Based data from over 6000 captive birds, estimated this a wide range fitness‐related traits. We found that, compared with ancestral allele A, inverted D had small additive beneficial male siring success female fecundity. These fitness‐enhancing may explain initial spread derived (allele frequency 53%). However, individuals were homozygous for slightly lower survival rate, which why not fixation. used individual‐based simulations examine how antagonistic behaves time. Our results indicate become stabilized at intermediate if additively effect size recessive weakly deleterious mutation, overall resulting net heterosis. Importantly, conclusion remains valid selection coefficients against DD (up lethality), suggesting conditions needed maintain frequently be met. also suggest our populations, (on captivity) is quite large enough prevent fixation long run. Estimates free‐living populations are validate these results.

Language: Английский

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Male zebra finches exposed to lead (Pb) during development have reduced volume of song nuclei, altered sexual traits, and received less attention from females as adults

Ecotoxicology and Environmental Safety, Journal Year: 2021, Volume and Issue: 210, P. 111850 - 111850

Published: Jan. 9, 2021

Lead (Pb) is a pervasive global contaminant that interferes with sensitive windows for neurological development and causes oxidative damage to tissues. The effects of moderate high exposure Pb have been well-studied in birds, but whether low-level early-life influences adult phenotype remains unclear. Female songbirds use male's song coloration discriminate between high- low-quality males. Therefore, if disrupts learning ability or shifts the allocation antioxidant pigments away from colorful secondary sexual traits, male birds exposed may be less attractive females. We developing zebra finches (Taeniopygia guttata) Pb-contaminated drinking water (100 1000 parts per billion [ppb]) after hatching (days 0–100). Once reached adulthood (120–150 days post hatch), we measured ability, bill cheek patches, volume nuclei brain. also female preference Pb-exposed males relative control Finally, motoric spatial cognitive performance assess traits differed their sensitivity exposure. Male ppb had impaired reduced nuclei, bills redness received attention Additionally, both did not affect task. Adult higher blood-Pb levels, though all cases levels were below 7.0 µg dL−1. This study suggests contributes deficits persist into indirectly influence fitness by altering reducing attractiveness.

Language: Английский

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Proximate Causes of Infertility and Embryo Mortality in Captive Zebra Finches

The American Naturalist, Journal Year: 2020, Volume and Issue: 196(5), P. 577 - 596

Published: July 20, 2020

AbstractSome species show high rates of reproductive failure, which is puzzling because natural selection works against such failure in every generation. Hatching common both captive and wild zebra finches (Taeniopygia guttata), yet little known about its proximate causes. Here we analyze data on performance (the fate >23,000 eggs) based up to 14 years breeding four finch populations. We find that virtually all aspects are negatively affected by inbreeding (mean r=-0.117); an early-starting, age-related decline r=-0.132); poor early-life nutrition r=-0.058). However, these effects together explain only 3% the variance infertility, offspring mortality, fecundity, fitness. In contrast, individual repeatability different fitness components varied between 15% 50%. As expected, found relatively low heritability (median: 7% phenotypic variation 29% individually repeatable variation). Yet some heritable appears be maintained antagonistic pleiotropy (negative genetic correlations) male traits female traits. The large amount unexplained suggests a potentially important role local dominance epistasis, including possibility segregating incompatibilities.

Language: Английский

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Occasional paternal inheritance of the germline-restricted chromosome in songbirds

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Jan. 28, 2021

Abstract All songbirds have one special accessory chromosome 1–4 , the so-called germline-restricted (GRC) 4–7 which is only present in germline cells and absent from all somatic tissues. Earlier work on zebra finch ( Taeniopygia guttata castanotis ) showed that GRC inherited through female line 4,6,8,9 – like mitochondrial DNA 7,9–12 eliminated sperm during spermatogenesis 5,7,9–11 . Here we show can also be paternally inherited. Confocal microscopy using GRC-specific FISH probes indicated a considerable fraction of heads (1-19%) ejaculates still contained GRC. In with these cytogenetic data, sequencing revealed individual males two families differed strongly consistently number GRCs their ejaculates. Examining captive-bred population hybrids subspecies T. g. descendants mitochondria mother but father. Moreover, haplotypes across nine different matrilines at best weak tendency to co-inherited mtDNA haplotypes. Within little variability, while was highly divergent. This suggests single haplotype has recently spread entire population, crossing matriline boundaries via paternal spillover. Our findings raise possibility certain could selfishly additional transmission, thereby outcompeting other were limited strict maternal inheritance, even if this partly detrimental organismal fitness.

Language: Английский

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