Sexually dimorphic DNA methylation and gene expression patterns in human first trimester placenta DOI Creative Commons
Tania L Gonzalez, Bryn E. Willson, Erica T. Wang

et al.

Biology of Sex Differences, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 16, 2024

Abstract Background Fetal sex and placental development impact pregnancy outcomes fetal–maternal health, but the critical timepoint of placenta establishment in first trimester is understudied human pregnancies. Methods Pregnant subjects were recruited late (weeks 10–14) at time chorionic villus sampling, a prenatal diagnostic test. Leftover tissue was collected stored until birth known, then DNA RNA isolated from singleton, normal karyotype pregnancies resulting live births. methylation measured with Illumina Infinium MethylationEPIC BeadChip array (n = 56). Differential analysis compared 25 females versus 31 males using generalized linear model on 743,461 autosomal probes. Gene expression differences analyzed RNA-sequencing 74). An integrated performed regression to correlate gene 51 overlapping placentas. Results Methylation identified 151 differentially methylated probes (DMPs) significant false discovery rate < 0.05, including 89 (59%) hypermethylated females. Probe cg17612569 ( GABPA , ATP5J ) most CpG site, males. There 11 regions affected by fetal sex, transcription factors ZNF300 ZNF311 significantly females, respectively. 152 genes sexually dimorphic 0.05. The DMPs associated 18 downregulation (P 0.05) direction hypermethylation, 2 0.05 CUB Sushi multiple domains 1, CSMD1 ). Both genes, as well Family With Sequence Similarity 228 Member A (FAM228A) showed correlation between expression, though FAM228A less dimorphic. Comparison other studies found that male-hypermethylated across gestation blood up adulthood. Conclusions Overall, differential small, there remain may be regulated through leading placenta.

Language: Английский

Exploratory analysis of differences at the transcriptional interface between the maternal and fetal compartments of the sheep placenta and potential influence of fetal sex DOI
Katherine M Halloran, Nadia Saadat, Brooke Pallas

et al.

Molecular and Cellular Endocrinology, Journal Year: 2025, Volume and Issue: 603, P. 112546 - 112546

Published: April 12, 2025

Language: Английский

Citations

0
High-throughput mRNA sequencing of human placenta shows sex differences across gestation DOI Creative Commons
Amy E Flowers, Tania L Gonzalez, Yizhou Wang

et al.

Placenta, Journal Year: 2024, Volume and Issue: 150, P. 8 - 21

Published: March 21, 2024

Language: Английский

Citations

2
Syncytiotrophoblast Markers Are Downregulated in Placentas from Idiopathic Stillbirths DOI Open Access
Sara Vasconcelos, Ioannis Moustakas, Miguel R. Branco

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5180 - 5180

Published: May 9, 2024

The trophoblast cells are responsible for the transfer of nutrients between mother and foetus play a major role in placental endocrine function by producing releasing large amounts hormones growth factors. Syncytiotrophoblast (STB), formed fusion mononuclear cytotrophoblasts (CTB), constitute interface essential all these functions. We performed transcriptome analysis human samples from two control groups—live births (LB), stillbirths (SB) with clinically recognised cause—and our study group, idiopathic (iSB). identified 1172 DEGs iSB, when comparing LB group; however, we compared iSB SB only 15 12 genes were down- upregulated respectively. An assessment commonly downregulated iSB. Among these, several syncytiotrophoblast markers, like PSG CSH families, as well ALPP, KISS1, CRH, significantly revealed underlying differences at molecular level involving syncytiotrophoblast. This suggests that defects syncytial layer may underlie unexplained stillbirths, therefore offering insights to improve clinical obstetrics practice.

Language: Английский

Citations

2
Sexually dimorphic DNA methylation and gene expression patterns in human first trimester placenta DOI Creative Commons
Tania L Gonzalez, Bryn E. Willson, Erica T. Wang

et al.

Biology of Sex Differences, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 16, 2024

Abstract Background Fetal sex and placental development impact pregnancy outcomes fetal–maternal health, but the critical timepoint of placenta establishment in first trimester is understudied human pregnancies. Methods Pregnant subjects were recruited late (weeks 10–14) at time chorionic villus sampling, a prenatal diagnostic test. Leftover tissue was collected stored until birth known, then DNA RNA isolated from singleton, normal karyotype pregnancies resulting live births. methylation measured with Illumina Infinium MethylationEPIC BeadChip array (n = 56). Differential analysis compared 25 females versus 31 males using generalized linear model on 743,461 autosomal probes. Gene expression differences analyzed RNA-sequencing 74). An integrated performed regression to correlate gene 51 overlapping placentas. Results Methylation identified 151 differentially methylated probes (DMPs) significant false discovery rate < 0.05, including 89 (59%) hypermethylated females. Probe cg17612569 ( GABPA , ATP5J ) most CpG site, males. There 11 regions affected by fetal sex, transcription factors ZNF300 ZNF311 significantly females, respectively. 152 genes sexually dimorphic 0.05. The DMPs associated 18 downregulation (P 0.05) direction hypermethylation, 2 0.05 CUB Sushi multiple domains 1, CSMD1 ). Both genes, as well Family With Sequence Similarity 228 Member A (FAM228A) showed correlation between expression, though FAM228A less dimorphic. Comparison other studies found that male-hypermethylated across gestation blood up adulthood. Conclusions Overall, differential small, there remain may be regulated through leading placenta.

Language: Английский

Citations

1