Three-dimensional quantitative micro-elastography reveals alterations in spatial elasticity patterns of follicles and corpora lutea in murine ovaries during ageing DOI Creative Commons
Anna Jaeschke, Matt S. Hepburn, Alireza Mowla

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Summary Fibrosis and tissue stiffening are hallmarks of ovarian ageing, linked to a decrease in fertility. However, the lack three-dimensional (3D) characterization ovary elasticity limits our understanding localized patterns their connection composition. Here, we developed an integrated approach link elasticity, volume, cell-matrix composition using quantitative micro-elastography (QME), label-free, non-invasive study 3D microscale conjunction with immunofluorescence microscopy. QME revealed distinct spatial compartments, namely follicles corpora lutea (CLs), local alterations different age cohorts. For example, CL significantly increased during ageing while follicle changed minimally. CLs showed size-dependent changes, exhibited variations correlated emergence theca cell layers development. These findings have potential guide development novel diagnostic tools identify therapeutic targets, improving women’s reproductive health longevity. Graphical Abstract

Language: Английский

Elastography in Reproductive Medicine, a Game-Changer for Diagnosing Polycystic Ovary Syndrome, Predicting Intrauterine Insemination Success, and Enhancing In Vitro Fertilization Outcomes: A Systematic Review DOI Creative Commons
Charalampos Voros, Antonia Varthaliti, Depy Mavrogianni

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 784 - 784

Published: March 24, 2025

Background: Elastography is an ultrasound-based imaging technology that allows for quantitative measurement of tissue stiffness and elasticity. In reproductive medicine, it a potential non-invasive method assessing ovarian activity, uterine contractility, endometrial receptivity. While conventional ultrasound provides anatomical vascular information, does not assess biomechanical properties, which are important understanding polycystic ovary syndrome (PCOS), predicting intrauterine insemination (IUI) success, determining receptivity in vitro fertilization (IVF). Methods: A systematic review was conducted accordance with the PRISMA principles, protocol recorded PROSPERO. comprehensive literature search across several databases to uncover studies used real-time elastography (RTE) or shear wave (SWE) PCOS diagnosis, IUI result prediction, evaluation IVF. The risk bias assessed using ROBINS-I technique. Results: Four fulfilled inclusion criteria. One study indicated patients had considerably increased stiffness, supports as diagnostic marker. Another found flexibility decreased contractility were related better outcomes. retrospective cohort research discovered non-uniform echogenicity no influence on IVF results. Furthermore, SWE successfully evaluated unexplained infertility, higher being reduced implantation potential. Conclusions: gives real-time, insights into biomechanics, applications infertility diagnosis ART improvement. However, absence defined procedures confirmed clinical criteria prevent its broad use. More large-scale prospective investigations required improve elastographic parameters define cutoffs

Language: Английский

Citations

0

Systemic low-dose anti-fibrotic treatment attenuates ovarian aging in the mouse DOI
Farners Amargant,

Carol Magalhaes,

Michele T. Pritchard

et al.

GeroScience, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

Language: Английский

Citations

3

Systemic low-dose anti-fibrotic treatment attenuates ovarian aging in the mouse DOI Creative Commons
Farners Amargant,

Carol Vieira,

Michele T. Pritchard

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

The female reproductive system is one of the first to age in humans, resulting infertility and endocrine disruptions. aging ovary assumes a fibro-inflammatory milieu which negatively impacts gamete quantity quality as well ovulation. Here we tested whether systemic delivery anti-inflammatory (Etanercept) or anti-fibrotic (Pirfenidone) drugs attenuates ovarian mice. We evaluated ability these decrease expression genes primary stromal cells. Whereas Etanercept did not block

Language: Английский

Citations

2

Flexural Eigenfrequency Analysis of Healthy and Pathological Tissues Using Machine Learning and Nonlocal Viscoelasticity DOI Creative Commons
Ali Farajpour, Wendy V. Ingman

Computers, Journal Year: 2024, Volume and Issue: 13(7), P. 179 - 179

Published: July 19, 2024

Biomechanical characteristics can be used to assist the early detection of many diseases, including breast cancer, thyroid nodules, prostate liver fibrosis, ovarian and tendon disorders. In this paper, a scale-dependent viscoelastic model is developed assess biomechanical behaviour biological tissues subject flexural waves. The nonlocal strain gradient theory, in conjunction with machine learning techniques such as extreme boosting, k-nearest neighbours, support vector machines, random forest, utilised develop computational platform for analysis. coupled governing differential equations are derived using Hamilton’s law. Transverse wave analysis conducted investigate different normal pathological human conditions fibrosis. Viscoelastic, gradient, effects describe impact fluid content, stiffness hardening caused by gradients components, softening associated nonlocality stress components within cells. integration continuum facilitates adoption practical applications allowing from clinical data, alongside intrinsic mechanical laws that govern responses.

Language: Английский

Citations

2

Mathematical Models for Ultrasound Elastography: Recent Advances to Improve Accuracy and Clinical Utility DOI Creative Commons
Ali Farajpour, Wendy V. Ingman

Bioengineering, Journal Year: 2024, Volume and Issue: 11(10), P. 991 - 991

Published: Sept. 30, 2024

Changes in biomechanical properties such as elasticity modulus, viscosity, and poroelastic features are linked to the health status of biological tissues. Ultrasound elastography is a non-invasive imaging tool that quantitatively maps these characteristics for diagnostic treatment monitoring purposes. Mathematical models essential ultrasound they convert raw data obtained from tissue displacement caused by waves into images observed clinicians. This article reviews available mathematical frameworks continuum mechanics extracting tissues elastography. Continuum-mechanics-based approaches classical viscoelasticity, elasticity, poroelasticity models, well nonlocal continuum-based described. The accuracy can be increased with recent advancements modelling techniques including hyperelasticity, biphasic theory, inversion-based incorporating scale effects. However, time taken clinical increases more complex this major challenge expanding utility As we strive provide most accurate patients, further research needed refine incorporation workflow.

Language: Английский

Citations

0

Three-dimensional quantitative micro-elastography reveals alterations in spatial elasticity patterns of follicles and corpora lutea in murine ovaries during ageing DOI Creative Commons
Anna Jaeschke, Matt S. Hepburn, Alireza Mowla

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Summary Fibrosis and tissue stiffening are hallmarks of ovarian ageing, linked to a decrease in fertility. However, the lack three-dimensional (3D) characterization ovary elasticity limits our understanding localized patterns their connection composition. Here, we developed an integrated approach link elasticity, volume, cell-matrix composition using quantitative micro-elastography (QME), label-free, non-invasive study 3D microscale conjunction with immunofluorescence microscopy. QME revealed distinct spatial compartments, namely follicles corpora lutea (CLs), local alterations different age cohorts. For example, CL significantly increased during ageing while follicle changed minimally. CLs showed size-dependent changes, exhibited variations correlated emergence theca cell layers development. These findings have potential guide development novel diagnostic tools identify therapeutic targets, improving women’s reproductive health longevity. Graphical Abstract

Language: Английский

Citations

0