The application value of bacteriophage in patients with severe drug-resistant bacterial infections DOI
Juan Chen, Man Huang

Journal of Translational Critical Care Medicine, Journal Year: 2024, Volume and Issue: 6(4)

Published: Sept. 19, 2024

The emergence of antimicrobial resistance (AMR) represents one the most significant global public health threats, imposing substantial economic burdens. To address this critical issue resistance, promising alternative strategies for treating resistant bacterial infections is bacteriophage therapy. Bacteriophages exhibit high specificity towards bacteria, capable lysing and killing specific strains. Phage cocktails can broaden spectrum activity, contribute to target multiple types bacteria. Personalized therapy, tailored individual patients, enhances precise targeting pathogens. Increasing evidence supports feasibility phage-antibiotic combinations in multidrug-resistant Furthermore, genetic engineering, including technologies like clustered regularly interspaced short palindromic repeats (CRISPR)-Cas, has advanced development engineering phages, providing novel ways kill hosts. This review discusses research progress refractory caused by advantages limitations phage highlights clinical studies case reports various treatment modalities.

Language: Английский

Isolation, characterization, and genomic analysis of a novel bacteriophage vB_Kp_XP4 targeting hypervirulent and multidrug-resistant Klebsiella pneumoniae DOI Creative Commons
Xinya Peng,

Junliang Chang,

Hongxia Zhang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Introduction Hypervirulent and multidrug-resistant Klebsiella pneumoniae (hvKP MDR-KP) are significant public health threats. This study aimed to isolate a lytic bacteriophage targeting these high-risk strains, systematically characterize its biological properties, genomic features, therapeutic efficacy, establish foundation for clinical phage therapy novel antimicrobial development. Methods The vB_Kp_XP4 was isolated from river water using the double-layer agar plate method with clinically strain P4 as host. Morphology analyzed via transmission electron microscopy (TEM). Host range, pH, thermal stability were assessed spot assays OD 630 measurements. One-step growth curves determined latent period burst size. Whole-genome sequencing phylogenetic analysis performed. Therapeutic efficacy safety evaluated in Galleria mellonella infection model. Results TEM revealed Phage tailed an icosahedral head long, flexible tail. It lysed hvKP (carrying rmp , peg iuc iro genes) MDR-KP (resistant carbapenems, fluoroquinolones, etc.), optimal MOI of 0.1 <10 minutes. Stability maintained at pH 4–11 ≤70°C. linear double-stranded DNA genome 44,344 bp G+C content 53.80%. comprised 54 coding sequences lacked lysogenic, virulence, or antibiotic resistance genes. Phylogenetic positioned species within genus Drulisvirus family Autographiviridae . In model, prolonged survival P4-infected larvae ( P < 0.001) Conclusion exhibits high stability, specificity, potent activity, no undesirable genes, demonstrating effective vivo suggest potential applications against infections. presence multiple halos during plaque formation further enhances research value. complete sequence has been submitted GenBank under accession number PP663283.

Language: Английский

Citations

0
Opportunities and challenges in phage therapy for cardiometabolic diseases DOI
Koen Wortelboer, Hilde Herrema

Trends in Endocrinology and Metabolism, Journal Year: 2024, Volume and Issue: 35(8), P. 687 - 696

Published: April 17, 2024

Language: Английский

Citations

3
Phage-liposome nanoconjugates for orthopedic biofilm eradication DOI Creative Commons
Lei Wang, Tamta Tkhilaishvili, Zheng Jiang

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 376, P. 949 - 960

Published: Nov. 6, 2024

Infection by multidrug-resistant (MDR) bacteria has become one of the biggest threats to public health worldwide. One reason for difficulty in treatment is lack proper delivery strategies into MDR bacterial biofilms, where thick extracellular polymeric substance (EPS) layer impedes penetration antibiotics and nanoparticles. Here, we propose a novel bioactive nanoconjugate drug-loaded liposomes bacteriophages targeted eradication biofilms orthopedic infections. Phage Sb-1, which ability degrade EPS, was conjugated with antibiotic-loaded liposomes. Upon encountering biofilm, phage Sb-1 degrades EPS structure, thereby increasing sensitivity allowing penetrate deeply biofilm. As result, effective removal biofilm achieved low dose antibiotics, proved this study both vitro vivo investigations. Notably, rat prosthetic joint infection (PJI) model, found that liposome-phage nanoconjugates could effectively decrease load infected area significantly promote osteomyelitis recovery. It therefore believed conjugation bacteriophage open new possibilities infections, possibly other infections deep tissues.

Language: Английский

Citations

3
Phage Therapy as a Rescue Treatment for Recurrent Pseudomonas aeruginosa Bentall Infection DOI Creative Commons

Victor Eiferman,

Pierre-Adrien Vion,

Alexandre Bleibtreu

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(1), P. 123 - 123

Published: Jan. 17, 2025

Phage therapy is experiencing renewed interest, particularly for antibiotic-resistant infections, and may also be useful difficult-to-treat cases where surgery to remove foreign infected material deemed too risky. We report a case of recurrent Pseudomonas aeruginosa endocarditis with Bentall infection treated successfully combination antibiotics phages.

Language: Английский

Citations

0
Phage susceptibility testing methods or ‘phagograms’: where do we stand and where should we go? DOI
Camille Kolenda,

Julie Jourdan,

Tiphaine Roussel-Gaillard

et al.

Journal of Antimicrobial Chemotherapy, Journal Year: 2024, Volume and Issue: 79(11), P. 2742 - 2749

Published: Sept. 13, 2024

Abstract Phage therapy is a highly promising approach to address the challenge that presented by global burden of antimicrobial resistance. Given natural specificity phages, phage susceptibility testing (PST) prerequisite for successful personalized therapy, allowing selection active phages from large and diverse collections. However, issue an easy-to-use standardized technique remains. In this review, we describe principles, advantages drawbacks two routinely used PST techniques: plaque growth kinetic assays. These are labour-intensive time-consuming methods require automation one or more steps, including preparation test panels, incubation, reading analysis results. addition automation, there urgent need establish reference method enable efficient techniques therapeutic phages. We discuss knowledge gaps parameters be investigated work towards goal.

Language: Английский

Citations

2
A comparison of phage susceptibility testing with two liquid high-throughput methods DOI Creative Commons
Krupa Parmar, Joseph Fackler,

Zuriel Rivas

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 7, 2024

Phage therapy is a promising antibacterial strategy, especially given that drug-resistant bacterial infections are escalating worldwide. Because phages not active against all strains of species, being considered for therapeutic use would ideally be tested isolates from individual patients prior to administration. Standardized, clinically validated phage susceptibility testing (PST) methods needed assessing

Language: Английский

Citations

1
Rapid resistance evolution against phage cocktails DOI Creative Commons
Baltus A. van der Steen, Matti Gralka,

Yuval Mulla

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 27, 2024

When bacteria are treated with multiple antibiotics simultaneously, resistance is exceedingly unlikely to evolve. In stark contrast, against phages frequently arises during therapy. Why does multi-phage cocktails evolve so easily? Using a mathematical model, we show how the bacterial evolutionary dynamics and phage replicative uniquely intertwine, facilitating rapid evolution of resistance. As different replicate become inhibitory at varying time points, can sequentially acquire rather than simultaneously – increasing chance multi-resistance by orders magnitude. Additionally, identify regime where robustly prevented. Our findings provide framework for rational design minimize development.

Language: Английский

Citations

1
Lytic Spectra of Tailed Bacteriophages: A Systematic Review and Meta-Analysis DOI Creative Commons
Ivan M. Pchelin,

Andrei V. Smolensky,

Daniil V. Azarov

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(12), P. 1879 - 1879

Published: Dec. 4, 2024

As natural predators of bacteria, tailed bacteriophages can be used in biocontrol applications, including antimicrobial therapy. Also, phage lysis is a detrimental factor technological processes based on bacterial growth and metabolism. The spectrum bacteria interact with known as the host range. Phage science produced vast amount range data. However, there has been no attempt to analyse these data from viewpoint modern taxonomy. Here, we performed meta-analysis spotting plaquing obtained strains production species. main metric our study was value calculated ratio lysed number tested strains. We found boundary between narrow broad ranges phages taken whole. Family-level groups strictly lytic had significantly different median values 0.18 (Drexlerviridae) 0.70 (Herelleviridae). In Escherichia coli phages, were associated decreased efficiency plating. Bacteriophage morphology, genome size, tRNA-coding genes genomes did not correlate values. From perspective species, varied 0.04 infecting Acinetobacter baumannii 0.73 Staphylococcus aureus phages. Taken together, results imply that taxonomy their hosts predictive intraspecies ranges.

Language: Английский

Citations

1
Characterization of novel phage pK3–24 targeting multidrug-resistant Klebsiella pneumoniae and its therapeutic efficacy in Galleria mellonella larvae DOI Creative Commons
Junxia Feng, Xiaohu Cui, Bing Du

et al.

Virus Research, Journal Year: 2024, Volume and Issue: 350, P. 199481 - 199481

Published: Oct. 20, 2024

Klebsiella pneumoniae is a common, conditionally pathogenic bacterium that often has multidrug-resistant phenotype, leading to failure of antibiotic therapies. It can therefore induce serious diseases, including community-acquired pneumonia and bloodstream infections. As an emerging alternative antibiotics, phages are considered key solving the problem drug-resistant bacterial Here, we report novel phage, pK3-24, mainly targets ST447 K. pneumoniae. Phage pK3-24 T7-like short-tailed phage with fast adsorption capacity forms translucent plaques halos on lawns. The optimal multiplicity infection (MOI) 0.01, average burst size 50 PFU/mL. shows environmental stability, surviving at below °C pH values 6-10. double-stranded DNA genome 40,327 bp carries no antibiotic-resistance, virulence, or lysogeny genes. Phylogenetic analysis assigned genus Przondovirus as new species. inhibited production biofilm. Moreover, treatment doses MOI > 1 effectively reduced mortality Galleria mellonella larvae infected

Language: Английский

Citations

1
Beyond Antibiotics: Exploring the Potential of Bacteriophages and Phage Therapy DOI Open Access
Sudhangshu Kumar Biswas,

Md. Mehedi Hasan Sumon,

Sabbir Ahmed

et al.

PHAGE, Journal Year: 2024, Volume and Issue: 5(4), P. 186 - 202

Published: Oct. 24, 2024

The escalating global threat of antibiotic resistance has prompted a critical need for innovative approaches to bacterial infection treatment. In terms management, bacterial-associated disorders have reached point in the world due advent drug-resistant types bacteria. Nonetheless, continued bacteriophage research presents promising frontier battle against infections. this study, we explored effectiveness therapy antibiotic-resistant According our viewpoint, phage could replace antibiotics safety, efficacy, and specificity. Furthermore, offers versatility administering single phage, products, or modified various resistant Moreover, Phage cocktails, with their synergistic combinations, showcase holistic approach, mitigating risk expanding treatment possibilities. Similarly, synergy between phages holds promise overcoming forging path toward more effective sustainable antimicrobial strategies. review provides insights into transformative impact on current paradigms, its application methodological challenges limitations, as well scientists policymakers best areas study order combat resistance.

Language: Английский

Citations

1