When bigger is better: utilizing large animal models in vein graft surgery to gain insights into endothelial-to-mesenchymal transition DOI
Clarissa Becher, Marie‐José Goumans, Gonzalo Sánchez‐Duffhues

et al.

Cardiovascular Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Language: Английский

Reconceptualizing Endothelial-to-mesenchymal transition in atherosclerosis: Signaling pathways and prospective targeting strategies DOI Creative Commons

Nanlin You,

Guohao Liu, Mengchen Yu

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The modification of endothelial cells (ECs) biological function under pathogenic conditions leads to the expression mesenchymal stromal (MSCs) markers, defined as endothelial-to-mesenchymal transition (EndMT). Invisible in onset and slow progression, atherosclerosis (AS) is a potential contributor various atherosclerotic cardiovascular diseases (ASCVD). By triggering AS, EndMT, "initiator" induces progression ASCVD such coronary heart disease (CHD) ischemic cerebrovascular (ICD), with serious clinical complications myocardial infarction (MI) stroke. In-depth research pathomechanisms EndMT identification targeted therapeutic strategies hold considerable value for prevention treatment ASCVD-associated delayed EndMT. Although previous studies have progressively unraveled complexity its pathogenicity triggered by alterations vascular microenvironmental factors, systematic descriptions most recent roles strategies, their future directions are scarce.

Language: Английский

Citations

2
Uncovering endothelial to mesenchymal transition drivers in atherosclerosis via multi-omics analysis DOI Creative Commons
Qingyan Huang, Yuhong Gan, Xiaoqi Zheng

et al.

BMC Cardiovascular Disorders, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 17, 2025

This study aimed to identify novel candidates that regulate Endothelial mesenchymal transition(EndMT) in atherosclerosis by integrating multi-omics data. The single-cell RNA sequencing (scRNA-seq) dataset GSE159677, bulk RNA-seq GSE118446 and microarray GSE56309 were obtained from the Gene Expression Omnibus (GEO) database. uniform manifold approximation projection (UMAP) used for downscaling cluster identification. Differentially expressed genes (DEGs) analyzed using limma package. Functional enrichment analysis was applied DAVID functional annotation tool. Quantitative real-time polymerase chain reaction (qPCR) western blotting further validation. Nine endothelial cell (EC) clusters identified human plaques, with EC 5 exhibiting an EndMT phenotype. intersection of common DEGs vitro models revealed seven candidates: PTGS2, TPM1, SERPINE1, FN1, RASD1, SEMA3C, ESM1. Validation these findings carried out through qPCR analysis. Through integration data bioinformatics methods, our

Language: Английский

Citations

1
Endothelial-mesenchymal crosstalk drives osteogenic differentiation of human osteoblasts through Notch signaling DOI Creative Commons

Perepletchikova Daria,

Polina Kuchur,

Basovich Liubov

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 19, 2025

Angiogenesis and osteogenesis are closely interrelated. The interaction between endothelial bone-forming cells, such as osteoblasts, is crucial for normal bone development repair. Juxtacrine paracrine mechanisms play key roles in cell differentiation towards the osteogenic direction, assuming direct effect of endothelium on differentiation. However, this interplay have yet to be thoroughly studied. Isolated cells (EC) from human umbilical vein osteoblasts (OB) epiphysis femur or tibia were cultured indirect (separated by membrane) contact vitro under conditions. Osteogenic was verified RT-PCR, alizarin red staining. Shotgun proteomics RNA-sequencing used compare both EC OB different co-culture conditions assess EC-OB interplay. To verify role Notch signaling, experiments with modulation performed lentiviral transduction further co-cultivation OB. Additionally, assessed RNA-sequencing. opposite effects depending In contact, enhance differentiation, but cultures, suppress it. Our proteotranscriptomic analysis revealed that osteosuppressive related action factors secreted EC, while osteoinductive properties mediated signaling pathway, which can activated only upon a physical Indeed, co-culture, knockdown Notch1 Notch3 receptors has an inhibitory whereas activation intracellular domain either inductive data indicate dual regulating highlight unique pathway inducing during cell-to-cell interactions. findings study emphasize importance intercellular communication regulation osteoblast maintenance.

Language: Английский

Citations

1
Atherosclerosis in diabetes mellitus: novel mechanisms and mechanism-based therapeutic approaches DOI
Abdul Waheed Khan, Karin Jandeleit‐Dahm

Nature Reviews Cardiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Language: Английский

Citations

0
An integrated and multifunctional homemade cell sensor platform based on Si-d-CQDs and CRISPR-Cas12a for CD31 detection during endothelial-to-mesenchymal transition DOI

Guolin Yuan,

Dongliang Cheng, Jian Huang

et al.

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127612 - 127612

Published: Jan. 28, 2025

Language: Английский

Citations

0
Cardiotoxicity Beyond Cardiomyocytes—Focus on the Role of Cardiac Fibroblasts and Endothelial Cells DOI Creative Commons
Wouter C. Meijers,

Laura I. Yousif,

C. Pieters

et al.

Heart Lung and Circulation, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Isoforskolin, adenylate cyclase agonist, inhibits endothelial-to-mesenchymal transition in atherosclerosis DOI

Yiming Ma,

Yunke Shi,

Jinping Lun

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 139, P. 156520 - 156520

Published: Feb. 16, 2025

Language: Английский

Citations

0
Characterization of the Temporal Dynamics of the Endothelial–Mesenchymal-like Transition Induced by Soluble Factors from Dengue Virus Infection in Microvascular Endothelial Cells DOI Open Access
Jenny Paola Alfaro-García, Carlos A. Orozco, Julián Andrés Sánchez-Rendón

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2139 - 2139

Published: Feb. 27, 2025

Dengue virus (DV) infection poses a severe life-threatening risk in certain cases. This is mainly due to endothelial dysregulation, which causes plasma leakage and hemorrhage. However, the etiology of DV-induced dysregulation remains incompletely understood. To identify potential mechanisms caused by DV, effects conditioned media from (CMDV) on mechanics transcriptional profile cells were examined using permeability assays, atomic force microscopy, In-Cell Western blot silico transcriptomics. Exposure HMEC-1 CMDV increased cellular stiffness. It also induced expression key proteins associated with endothelial-to-mesenchymal transition (EndMT). These data support notion that DV promotes dysfunction triggering programs compromise barrier function. Understanding molecular underlying crucial for developing targeted therapeutic strategies mitigate outcomes dengue infection.

Language: Английский

Citations

0
Notch Signaling as a Way to Control the Endothelial-To-Mesenchymal Transition DOI

D. A. Evensen,

Daria Smirnova, A. I. Belyaeva

et al.

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 61(1), P. 313 - 327

Published: Jan. 1, 2025

Language: Английский

Citations

0
Opportunities and challenges for the use of human samples in translational cardiovascular research: a scientific statement of the ESC Working Group on Cellular Biology of the Heart, the ESC Working Group on Cardiovascular Surgery, the ESC Council on Basic Cardiovascular Science, the ESC Scientists of Tomorrow, the European Association of Percutaneous Cardiovascular Interventions of the ESC, and the Heart Failure Association of the ESC DOI Creative Commons
Sean M. Davidson, Ioanna Andreadou, Charalambos Antoniades

et al.

Cardiovascular Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Animal models offer invaluable insights into disease mechanisms but cannot entirely mimic the variability and heterogeneity of human populations, nor increasing prevalence multi-morbidity. Consequently, employing samples-such as whole blood or fractions, valvular vascular tissues, myocardium, pericardium, human-derived cells-is essential for enhancing translational relevance cardiovascular research. For instance, myocardial tissue slices, which preserve crucial structural functional characteristics heart, can be used in vitro to examine drug responses. Human serves a rich source biomarkers, including extracellular vesicles, various types RNA (miRNA, lncRNA, circRNAs), circulating inflammatory cells, endothelial colony-forming facilitating detailed studies diseases. Primary cardiomyocytes cells isolated from tissues are mechanistic investigations vitro. In cases where these unavailable, induced pluripotent stem serve effective substitutes, albeit with specific limitations. However, use samples presents challenges such ethical approvals, procurement storage, patient genetics treatment regimens, selection appropriate control samples. Biobanks central efficient scarce valuable resources. This scientific statement discusses opportunities implement research within clinical contexts, offers practical framework acquiring utilizing different materials, examples sample applications diseases, providing resource clinicians, basic scientists engaged

Language: Английский

Citations

0