Does Crayfish Molting Affect Environmental DNA Detectability? DOI

Samantha M. García,

Amanda N. Curtis,

Jordan H. Hartmen

et al.

Freshwater Crayfish, Journal Year: 2024, Volume and Issue: 29(1), P. 37 - 47

Published: Dec. 30, 2024

Abstract While previous studies have explored the impact of behavior and life history on environmental DNA (eDNA), little research has been conducted molting eDNA detectability particle size for aquatic arthropods like crayfish. To address this knowledge gap, we a study examining how affects over range sizes using marbled crayfish (Procambarus virginalis Lyko, 2017). We collected water samples from aquariums at pre- post-molt time intervals filtered our sequential filtration large to small filter pore sizes. found that had weak negative effect detectability, P. was most detectable throughout largest (5.0 μm) regardless molt status. Additionally, spent in strong, positive virginalis. Given crayfish, suggest researchers align their sampling efforts with other seasonal events, reproduction or egg extrusion, demonstrated improve detectability.

Language: Английский

Environmental Adaptation of Genetically Uniform Organisms with the Help of Epigenetic Mechanisms—An Insightful Perspective on Ecoepigenetics DOI Creative Commons
Günter Vogt

Epigenomes, Journal Year: 2022, Volume and Issue: 7(1), P. 1 - 1

Published: Dec. 26, 2022

Organisms adapt to different environments by selection of the most suitable phenotypes from standing genetic variation or phenotypic plasticity, ability single genotypes produce in environments. Because near identity, asexually reproducing populations are particularly for investigation potential and molecular underpinning latter alternative depth. Recent analyses on whole-genome scale differently adapted clonal animals plants demonstrated that epigenetic mechanisms such as DNA methylation, histone modifications non-coding RNAs among pathways supporting plasticity is used stably Case studies revealed habitat-specific fingerprints were maintained over subsequent years pointing at existence ecotypes. Environmentally induced epimutations corresponding gene expression changes provide an ideal means fast directional adaptation changing new conditions, because they can synchronously alter many population members. microorganisms inclusive human pathogens also exploit epigenetically mediated environmental adaptation, this phenomenon considered a universal biological principle. The production same sequence response cues provides mechanistic explanation “general-purpose genotype hypothesis” “genetic paradox invasions”.

Language: Английский

Citations

15
Mixed Patterns of Intergenerational DNA Methylation Inheritance in Acropora DOI Creative Commons
Christopher R. Peterson, Carly B. Scott, Rashin Ghaffari

et al.

Molecular Biology and Evolution, Journal Year: 2024, Volume and Issue: 41(2)

Published: Jan. 18, 2024

Abstract For sessile organisms at high risk from climate change, phenotypic plasticity can be critical to rapid acclimation. Epigenetic markers like DNA methylation are hypothesized as mediators of plasticity; is associated with the regulation gene expression, change in response ecological cues, and a proposed basis for inheritance acquired traits. Within reef-building corals, gene-body (gbM) stressors. If coral transmissible across generations, this could potentially facilitate acclimation environmental change. We investigated heritability Acropora, stony coral. Two Acropora millepora two selago adults were crossed, producing eight offspring crosses (four hybrid, each species). used whole-genome bisulfite sequencing identify methylated loci allele-specific alignments quantify per-locus inheritance. heritable, differential (DM) between parents should equal DM paired alleles given locus. found mixture heritable nonheritable loci, portions ranging 44% 90% among crosses. gBM was more than intergenic methylation, most had consistent degree (i.e. deviation parental similar magnitude direction). Our results provide evidence that inherited but heterogenous throughout genome. Future investigations into heterogeneity its implications will important understanding potential capability intergenerational reef building corals.

Language: Английский

Citations

1
Elucidating the role of DNA methylation in low-salinity adaptation of the swimming crab Portunus trituberculatus DOI

Junyang Guo,

Jianjian Lv,

Dongfang Sun

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 595, P. 741707 - 741707

Published: Oct. 4, 2024

Language: Английский

Citations

1
Expression of C/EBP and Kr-h1 transcription factors under immune stimulation in the noble crayfish DOI Creative Commons
Ljudevit Luka Boštjančić, Paula Dragičević, Lena Bonassin

et al.

Gene, Journal Year: 2024, Volume and Issue: 929, P. 148813 - 148813

Published: Aug. 1, 2024

Transcription factors (TFs) have an important role in the regulation of gene expression network. The TFs immune response freshwater crayfish is poorly understood, but leveraging regulatory mechanisms could augment resistance against invasive oomycete pathogen, Aphanomyces astaci. Previous studies indicated that CCAAT/enhancer-binding protein (C/EBP) and putative Krüppel homolog-1 (Kr-h1) might play a stress noble (Astacus astacus). Here, we aimed to further characterise these two products gain better understanding their evolutionary origin, domain organisation patterns across different tissues. Furthermore, conducted stimulation experiment observe potential changes C/EBP Kr-h1 under challenge Our results showed both are closely related other C/EBPs Kr-h1s Malacostraca. Gene analysis revealed present all analysed tissues, with higher gills abdominal muscle. Immune laminarin (mimicking β-1-3-glucan cell wall) activation system, overall increase total haemocyte count (THC) compared untreated control buffered saline (CBS) treatment. On level, up-regulation was detected treated group hepatopancreas heart, while no were observed for gene. indicate early change multiple tissues during suggest its involvement crayfish.

Language: Английский

Citations

0
Embodied Computational Evolution: A Model for Investigating Randomness and the Evolution of Morphological Complexity DOI Creative Commons
Eric Aaron, John H. Long

Integrative Organismal Biology, Journal Year: 2024, Volume and Issue: 6(1)

Published: Jan. 1, 2024

For an integrated understanding of how evolutionary dynamics operate in parallel on multiple levels, computational models can enable investigations that would be otherwise infeasible or impossible. We present one modeling framework,

Language: Английский

Citations

0
Epigenetic Resources: Gaps in Aquatic Animal Germplasm Research DOI
Fangcheng Li, Junfang Zhang, Xiangbing Cheng

et al.

Reviews in Aquaculture, Journal Year: 2024, Volume and Issue: 17(1)

Published: Dec. 5, 2024

ABSTRACT Aquatic animal germplasm research plays a vital role in biodiversity conservation and sustainable aquaculture. The traditional view is that diversity of resources commonly attributed to genetic variation. However, recent studies transgenerational epigenetics have shown epigenetic information can also be passed down offspring, which consistent with the definition germplasm. Therefore, it necessary define information, such as DNA methylation, histone modifications, ncRNAs, through generations, incorporate them into concept Germplasm should include not only but resources. Epigenetic variation arise from genetic, environmental, or stochastic factors. Genetic co‐determine phenotypic traits. It has been demonstrated inherited across generations aquatic animals. modification, ncRNAs are involved addressing environmental challenges, maintaining diversity, improving breeding strategies, combating inbreeding decline, age determination. future on important for conservation, development, utilization. This review proposes new theoretical framework elucidate place collects articles past 8 years explore inheritance Integrating enhances our understanding population dynamics, adaptation, evolutionary processes, thereby informing strategies enhancing aquaculture practices. By considering both resources, we address challenges facing more comprehensively.

Language: Английский

Citations

0
Does Crayfish Molting Affect Environmental DNA Detectability? DOI

Samantha M. García,

Amanda N. Curtis,

Jordan H. Hartmen

et al.

Freshwater Crayfish, Journal Year: 2024, Volume and Issue: 29(1), P. 37 - 47

Published: Dec. 30, 2024

Abstract While previous studies have explored the impact of behavior and life history on environmental DNA (eDNA), little research has been conducted molting eDNA detectability particle size for aquatic arthropods like crayfish. To address this knowledge gap, we a study examining how affects over range sizes using marbled crayfish (Procambarus virginalis Lyko, 2017). We collected water samples from aquariums at pre- post-molt time intervals filtered our sequential filtration large to small filter pore sizes. found that had weak negative effect detectability, P. was most detectable throughout largest (5.0 μm) regardless molt status. Additionally, spent in strong, positive virginalis. Given crayfish, suggest researchers align their sampling efforts with other seasonal events, reproduction or egg extrusion, demonstrated improve detectability.

Language: Английский

Citations

0