Hydrogel Containing Biogenic Silver Nanoparticles and Origanum vulgare Essential Oil for Burn Wounds: Antimicrobial Efficacy Using Ex Vivo and In Vivo Methods Against Multidrug-Resistant Microorganisms DOI Creative Commons
Angela Hitomi Kimura, Débora Dahmer,

Luana Ayumi Isawa

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 503 - 503

Published: April 10, 2025

Background/Objectives: Wounds from burns are susceptible to infections, allowing multidrug-resistant microorganisms complicate treatments and patient recovery. This highlights the development of new strategies control these microorganisms. work evaluated antibacterial activity hydrogels containing biogenic silver nanoparticles (bio-AgNP) Origanum vulgare essential oil (OEO) against bacteria. Methods: The formulations were subjected organoleptic, pharmacotechnical, stability characterization antimicrobial assessment by time–kill tests alternative methods, an ex vivo model using porcine skin, in Galleria mellonella. Results: All maintained their after thermal stress. hydrogel bio-AgNP + OEO 1% (HAgNP OEO1) presented bactericidal effectiveness, within 2 h, both Gram-positive Gram-negative bacteria test. For testing, HAgNP OEO1 was compared with sulfadiazine (SS) base formulation. In test, SS showed a similar reduction superficial washing burn for S. aureus 999, while P. aeruginosa, more expressive treatment. tissue, treatment effective aeruginosa 1461, similarly effective. mellonella survival rates 48 h 84% group (base) 50% groups. Conclusions: study demonstrates that combining antimicrobials is microorganisms, offering promising infected burns.

Language: Английский

Galleria mellonella as a Suitable Model of Bacterial Infection: Past, Present and Future DOI Creative Commons
Guillaume Ménard, Astrid Rouillon, Vincent Cattoir

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2021, Volume and Issue: 11

Published: Dec. 22, 2021

The increasing interest for Galleria mellonella larvae as an infection model is evidenced by the number of papers reporting its use, which increases exponentially since early 2010s. This popularity was initially linked to limitation conventional animal models due financial, technical and ethical aspects. In comparison, alternative (e.g. using Caenorhabditis elegans, Drosophila melanogaster or G. mellonella) were cheap, simple use not limited regulation. Since then, similar results have been established with comparatively vertebrates, it more often used a robust per se, only murine model. review attempts summarize current knowledge supporting development this model, both on immunological microbiological For that, we focus investigation virulence new therapies most important pathogenic bacteria. We also discuss points out directions standardization, well recent advances perspectives monitoring host-pathogen interactions.

Language: Английский

Citations

124
The Virtuous Galleria mellonella Model for Scientific Experimentation DOI Creative Commons
Isa Serrano, Cláudia Verdial, Luís Tavares

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(3), P. 505 - 505

Published: March 3, 2023

The first research on the insect Galleria mellonella was published 85 years ago, and larva is now widely used as a model to study infections caused by bacterial fungal pathogens, for screening new antimicrobials, adjacent immune response in co-infections or host-pathogen interaction, well toxicity model. system of G. shows remarkable similarities with mammals. Furthermore, results from correlate positively mammalian models other invertebrate models. Unlike models, can withstand temperatures 37 °C, its handling experimental procedures are simpler. Despite having some disadvantages, virtuous vivo be preclinical studies, an intermediate between vitro great example how apply bioethics principle 3Rs (Replacement, Reduction, Refinement) animal experimentation. This review aims discuss progress model, highlighting key aspects use, including design considerations necessity standardize them. A different score “cocoon” category included Health Index Scoring System also proposed.

Language: Английский

Citations

40
Insects and Public Health: An Overview DOI Creative Commons
Simone Belluco, Michela Bertola, Fabrizio Montarsi

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(3), P. 240 - 240

Published: Feb. 27, 2023

Insects are, by far, the most common animals on our planet. The ubiquity and plethora of ecological niches occupied insects, along with strict sometimes forced coexistence between insects humans, make a target public health interest. This article reports negative aspects historically linked to as pests vectors diseases, describes their potential bioindicators environmental pollution, use food feed. Both positive impacts human animal need be addressed professionals who should aim strike balance within wide range conflicting goals in insect management, such regulating production, exploiting potential, protecting limiting impact humans. requires increased knowledge strategies preserve welfare. this paper is provide an overview traditional emerging topics bridging highlight for professionals, address these during work. present future role activities authorities regarding are analyzed.

Language: Английский

Citations

38
Immune functions of pattern recognition receptors in Lepidoptera DOI Creative Commons
Lin Zhao, Jinlan Niu,

Disong Feng

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: June 16, 2023

Pattern recognition receptors (PRRs), as the “sensors” in immune response, play a prominent role recognizing pathogen-associated molecular patterns (PAMPs) and initiating an effective defense response to pathogens Lepidoptera. It is becoming increasingly clear that damage-associated (DAMPs) normally physiological within cells; however, when exposed extracellular, they may become “part-time” critical signals of response. Based on research recent years, we review herein typical PRRs Lepidoptera, including peptidoglycan protein (PGRP), gram-negative binding (GNBP), β-1,3-glucan (βGRP), C-type lectin (CTL), scavenger receptor (SR). We also outline ways which DAMPs participate correlation between escape. Taken together, these findings suggest insect innate immunity be much greater than expected it possible recognize broader range signaling molecules.

Language: Английский

Citations

24
Galleria mellonella as a Model for the Study of Fungal Pathogens: Advantages and Disadvantages DOI Creative Commons
Andrea Giammarino, Nicolò Bellucci, Letizia Angiolella

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(3), P. 233 - 233

Published: March 7, 2024

The study of pathogenicity and virulence fungal strains, in vivo the preclinical phase, is carried out through use animal models belonging to various classes mammals (rodents, leproids, etc.). Although animals are functionally more similar humans, these studies have some limitations terms ethics (animal suffering), user-friendliness, cost-effectiveness, timing (physiological response time) logistics (need for adequately equipped laboratories). A good model must possess optimal characteristics be used, such as rapid growth, small size short life cycle. For this reason, insects, Galleria mellonella (Lepidoptera), Drosophila melanogaster (Diptera) Bombyx mori been widely used alternative non-mammalian models. Due their simplicity low cost, larvae G. represent an above all evaluate pathogens antifungal treatments (either single or combination with biologically active compounds). further advantage also represented by simple neuronal system limiting suffering itself, ability survive at near-body ambient temperatures well expression proteins able recognise combined following three R principles (replacement, refinement reduction). This review aims assess validity advantages disadvantages replacing mammalian experimentation.

Language: Английский

Citations

14
Genomic surveillance as a scalable framework for precision phage therapy against antibiotic-resistant pathogens DOI Creative Commons
Mihály Koncz, Tamás Stirling,

Hiba Hadj Mehdi

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

9
Galleria mellonella (Greater Wax Moth) as a Reliable Animal Model to Study the Efficacy of Nanomaterials in Fighting Pathogens DOI Creative Commons
Stefania Villani, Matteo Calcagnile, Christian Demitri

et al.

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(1), P. 67 - 67

Published: Jan. 3, 2025

The spread of multidrug-resistant microbes has made it necessary and urgent to develop new strategies deal with the infections they cause. Some these are based on nanotechnology, which revolutionized many fields in medicine. Evaluating safety efficacy antimicrobial requires testing animal models before being tested clinical trials. In this context, Galleria mellonella could represent a valid alternative traditional mammalian non-mammalian models, due its low cost, ease handling, valuable biological properties investigate host–pathogen interactions. purpose review is provide an updated overview literature concerning use G. larvae as model evaluate nanoparticles nanomaterials, particularly, those that used or under investigation combat microbial pathogens.

Language: Английский

Citations

1
Identifying virulence determinants of multidrug-resistant Klebsiella pneumoniae in Galleria mellonella DOI Creative Commons
Sebastian Bruchmann, Theresa Feltwell, Julian Parkhill

et al.

Pathogens and Disease, Journal Year: 2021, Volume and Issue: 79(3)

Published: Jan. 27, 2021

Infections caused by Klebsiella pneumoniae are a major public health threat. Extensively drug-resistant and even pan-resistant strains have been reported. Understanding K. pathogenesis is hampered the fact that murine models of infection offer limited resolution for non-hypervirulent which cause majority infections. The insect Galleria mellonella larva widely used alternative model organism bacterial pathogens. We performed genome-scale fitness profiling multidrug-resistant ST258 strain during G. mellonella, to determine if this suitable large-scale virulence factor discovery in pathogen. Our results demonstrated dominant role surface polysaccharides infection, with contributions from siderophores, cell envelope proteins, purine biosynthesis genes additional unknown function. Comparison hypervirulent strain, ATCC 43816, revealed substantial overlap important infection-related genes, as well putative factors specific ST258, reflecting strain-dependent effects. analysis also identified metalloregulatory protein NfeR (YqjI) virulence. Overall, study offers new insight into landscape pneumoniae, provides framework using highly flexible easily scalable dissect molecular mechanisms

Language: Английский

Citations

44
Natural Substrates and Culture Conditions to Produce Pigments from Potential Microbes in Submerged Fermentation DOI Creative Commons
Chatragadda Ramesh,

V. R. Prasastha,

Mekala Venkatachalam

et al.

Fermentation, Journal Year: 2022, Volume and Issue: 8(9), P. 460 - 460

Published: Sept. 14, 2022

Pigments from bacteria, fungi, yeast, cyanobacteria, and microalgae have been gaining more demand in the food, leather, textile industries due to their natural origin effective bioactive functions. Mass production of microbial pigments using inexpensive ecofriendly agro-industrial residues is current research low cost, origin, waste utilization, high pigment stimulating characteristics. A wide range substrates has employed submerged fermentation as carbon nitrogen sources enhance these microorganisms obtain required quantity pigments. Submerged proven yield when added with agro-waste residues. Hence, this review, aspects potential pigmented microbes such diversity, that stimulate a few under culture conditions, identification, ecological functions are detailed for benefit industrial personnel, researchers, other entrepreneurs explore multifaceted applications. In addition, some important covered herein disseminate knowledge.

Language: Английский

Citations

32
Microbiology's next top model: Galleria in the molecular age DOI Open Access
Hue Dinh, Lucie Semenec, Sheemal Kumar

et al.

Pathogens and Disease, Journal Year: 2021, Volume and Issue: unknown

Published: Jan. 19, 2021

Galleria mellonella has risen to fame as an invertebrate model organism given its ethical advantages, low maintenance costs, rapid reproduction time, short life cycle, high number of progeny, tolerance for human body temperatures, innate immune system and similarities mammalian host models. It is increasingly being utilised evaluate in vivo toxicity efficacy chemical compounds antimicrobials, modelling microbial (bacterial, fungal viral) pathogenicity assessing host-pathogen interaction during infection. During this molecular age genomic, transcriptomic, proteomic genetic manipulation approaches, our understanding interactions deepened from high-throughput studies performed G. mellonella. In review, we describe the use a broad range involving omics, drug resistance, functional analysis host-microbial community relationships. The future bright, with multitude new approaches uses clinical biotechnological on horizon.

Language: Английский

Citations

38