Horizontal transfer of antibiotic resistance genes in the human gut microbiome

Current Opinion in Microbiology, Journal Year: 2020, Volume and Issue: 53, P. 35 - 43

Published: Feb. 1, 2020

Language: Английский

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Composition and functions of bacterial membrane vesicles

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 21(7), P. 415 - 430

Published: March 17, 2023

Language: Английский

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Microbiota‐derived extracellular vesicles in interkingdom communication in the gut

Journal of Extracellular Vesicles, Journal Year: 2021, Volume and Issue: 10(13)

Published: Nov. 1, 2021

The intestine is fundamental in controlling human health. Intestinal epithelial and immune cells are continuously exposed to millions of microbes that greatly impact on intestinal barrier function. This microbial community, known as gut microbiota, now recognized an important partner the being actively contribute essential functions but also distal organs. In ecosystem, bidirectional microbiota-host communication does not involve direct cell contacts. Both microbiota host-derived extracellular vesicles (EVs) key players such interkingdom crosstalk. There accumulating body evidence bacterial secreted mediate by transporting delivering into host effector molecules modulate signalling pathways processes. Consequently, released may have great influence health disease. Here we review current knowledge EVs specifically highlight their role metabolism, integrity training.

Language: Английский

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Extracellular Vesicle Biogenesis and Functions in Gram-Positive Bacteria

Infection and Immunity, Journal Year: 2020, Volume and Issue: 88(12)

Published: Sept. 23, 2020

Extracellular vesicles (EVs) are membrane-derived lipid bilayers secreted by bacteria and eukaryotic cells. Bacterial membrane were discovered over 60 years ago have been extensively studied in Gram-negative bacteria. During their production, EVs loaded with proteins, nucleic acids, various compounds that subsequently released into the environment. Depending on packaged cargo, a broad spectrum of action involved pathogenesis, antibiotic resistance, nutrient uptake, acid transfer.

Language: Английский

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Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design

Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(13), P. 7957 - 8094

Published: June 15, 2021

Antimicrobial resistance is one of the major problems in current practical medicine. The spread genes coding for determinants among bacteria challenges use approved antibiotics, narrowing options treatment. Resistance to carbapenems, last resort a concern. Metallo-β-lactamases (MBLs) hydrolyze penicillins, and cephalosporins, becoming central this problem. These enzymes diverge with respect serine-β-lactamases by exhibiting different fold, active site, catalytic features. Elucidating their mechanism has been big challenge field that limited development useful inhibitors. This review covers exhaustively details active-site chemistries, diversity MBL alleles, against substrates, how information helped developing We also discuss here aspects critical understand success MBLs conferring resistance: molecular dissemination, cell physiology, from biogenesis processing involved transit periplasm, uptake Zn(II) ions upon metal starvation conditions, such as those encountered during an infection. In regard, chemical, biochemical microbiological provide integrative view knowledge MBLs.

Language: Английский

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Can Environmental RNA Revolutionize Biodiversity Science?

Trends in Ecology & Evolution, Journal Year: 2019, Volume and Issue: 34(8), P. 694 - 697

Published: May 31, 2019

Language: Английский

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Biological properties of plant-derived extracellular vesicles

Food & Function, Journal Year: 2019, Volume and Issue: 10(2), P. 529 - 538

Published: Jan. 1, 2019

Identification of active constituents our diet is crucial to understand the impact food on health, and disease development, for formulation functional nutraceuticals. Until now research into pharmacological properties components has focused vitamins, sterols, polyphenols, fiber, etc. But very recently, it been found that plants contain various types vesicles which are in contact with intestinal tract throughout lives. They participate tissue renewal processes modulate gut microbiota healthy subjects have important biological functions against inflammatory diseases (e.g.; colitis injury, liver steatosis) or cancers associated their specific lipid miRNA content. In addition, recent data suggested plant-derived nanovesicles would be excellent candidates delivery therapeutic agents anti-cancerous drugs, siRNAs) poorly soluble natural compounds curcumin), as they able cross mammalian barriers without inducing either an response necrosis, conversely conventional liposomes. It thus consider these new order evaluate potential health benefit food-derived technology.

Language: Английский

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Plant Extracellular Vesicles and Nanovesicles: Focus on Secondary Metabolites, Proteins and Lipids with Perspectives on Their Potential and Sources

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(7), P. 3719 - 3719

Published: April 2, 2021

While human extracellular vesicles (EVs) have attracted a big deal of interest and been extensively characterized over the last years, plant-derived EVs nanovesicles earned less attention remained poorly investigated. Although series investigations already revealed promising beneficial health effects drug delivery properties, adequate (pre)clinical studies are rare. This fact might be caused by lack sources with appropriate qualities. Our study introduces plant cell suspension culture as new well controllable source for EVs. Plant cells, cultured in vitro, release into growth medium which could harvested pharmaceutical applications. In this investigation we from distinct sources. findings regarding secondary metabolites indicate that these not packaged an active manner but enriched membrane when lipophilic enough, since apparently compounds were associated while more hydrophilic structures consistently found. addition, protein identification possible explanation mechanism EV wall passage plants, hydrolases like 1,3-β-glucosidases, pectinesterases, polygalacturonases, β-galactosidases β-xylosidase/α-L-arabinofuranosidase 2-like present facilitate transition. Further on, identified proteins cells secrete using similar mechanisms animal to exosomes microvesicles.

Language: Английский

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Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Journal of Extracellular Vesicles, Journal Year: 2021, Volume and Issue: 10(6)

Published: April 1, 2021

Abstract Cellular, inter‐organismal and cross kingdom communication via extracellular vesicles (EVs) is intensively studied in basic science with high expectation for a large variety of bio‐technological applications. EVs intrinsically possess many attributes drug delivery vehicle. Beyond the implications cell biology, academic industrial interests have increased last few years. Microalgae constitute sustainable renewable sources bioactive compounds range sectoral applications, including formulation health supplements, cosmetic products food ingredients. Here we describe newly discovered subtype derived from microalgae, which named nanoalgosomes. We isolated these nano‐objects cultures microalgal strains, marine photosynthetic chlorophyte Tetraselmis chuii , using differential ultracentrifugation or tangential flow fractionation focusing on nanosized small (sEVs). explore different biochemical physical properties show that nanoalgosomes are efficiently taken up by mammalian lines, confirming potential EVs. This first detailed description such membranous nanovesicles microalgae. With respect to other organisms, present several advantages microalgae natural source, could easily be scalable terms nanoalgosome production.

Language: Английский

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Extracellular vesicles: Emerging tools as therapeutic agent carriers

Acta Pharmaceutica Sinica B, Journal Year: 2022, Volume and Issue: 12(10), P. 3822 - 3842

Published: May 11, 2022

Extracellular vesicles (EVs) are secreted by both eukaryotes and prokaryotes, present in all biological fluids of vertebrates, where they transfer DNA, RNA, proteins, lipids, metabolites from donor to recipient cells cell-to-cell communication. Some EV components can also indicate the type status their parent serve as diagnostic targets for liquid biopsy. EVs natively carry or be modified contain therapeutic agents (e.g., nucleic acids, polysaccharides, small molecules) physical, chemical, bioengineering strategies. Due excellent biocompatibility stability, ideal nanocarriers bioactive ingredients induce signal transduction, immunoregulation, other effects, which targeted specific cell types. Herein, we review classification, intercellular communication, isolation, characterization strategies apply therapeutics. This focuses on recent advances applications carriers vitro research towards vivo animal models early clinical applications, using representative examples fields cancer chemotherapeutic drug, vaccine, infectious disease vaccines, regenerative medicine gene therapy. Finally, discuss current challenges therapeutics future development.

Language: Английский

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