Plant-derived exosome-like nanoparticles and their therapeutic activities

Asian Journal of Pharmaceutical Sciences, Journal Year: 2021, Volume and Issue: 17(1), P. 53 - 69

Published: July 10, 2021

Nanotechnologies have been successfully applied to the treatment of various diseases. Plant-derived exosome-like nanoparticles (PENs) are expected become effective therapeutic modalities for treating disease or in drug-delivery. PENs minimally cytotoxic healthy tissues, with which they show excellent biocompatibility, and biased towards tumors by targeting specific tissues through special endocytosis mechanisms. Thus, use these may expand scope drug therapies while reducing off-target effects. In this review, we summarize fundamental features bioactivities extracted from grape, grapefruit, ginger, lemon, broccoli discuss applications particles as therapeutics nanocarriers.

Language: Английский

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‘Green’ nanotherapeutics from tea leaves for orally targeted prevention and alleviation of colon diseases

Biomaterials, Journal Year: 2021, Volume and Issue: 279, P. 121178 - 121178

Published: Oct. 8, 2021

Language: Английский

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Plant-derived extracellular vesicles: a novel nanomedicine approach with advantages and challenges

Cell Communication and Signaling, Journal Year: 2022, Volume and Issue: 20(1)

Published: May 23, 2022

Abstract Background Many eukaryote cells produce membrane-enclosed extracellular vesicles (EVs) to establish cell-to-cell communication. Plant-derived EVs (P-EVs) contain proteins, RNAs, lipids, and other metabolites that can be isolated from the juice, flesh, roots of many species. Methods In present review study, we studied numerous articles over past two decades published on role P-EVs in plant physiology as well application these different diseases. Results Different types have been identified plants multiple functions including reorganization cell structure, development, facilitating crosstalk between fungi, immunity, defense against pathogens. Purified several edible species, are more biocompatible, biodegradable, extremely available plants, making them useful for cell-free therapy. Emerging evidence clinical preclinical studies suggest benefits conventional synthetic carriers, opening novel frontiers drug-delivery system. Exciting new opportunities, designing drug-loaded improve systems, already being examined, however translation P-EVs-based therapies faces challenges. Conclusion hold great promise treatment addition, despite enthusiastic results, further scrutiny should focus unravelling detailed mechanism behind biogenesis trafficking their therapeutic applications.

Language: Английский

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Message in a Bubble: Shuttling Small RNAs and Proteins Between Cells and Interacting Organisms Using Extracellular Vesicles

Annual Review of Plant Biology, Journal Year: 2021, Volume and Issue: 72(1), P. 497 - 524

Published: June 17, 2021

Communication between plant cells and interacting microorganisms requires the secretion uptake of functional molecules to from extracellular environment is essential for survival both plants their pathogens. Extracellular vesicles (EVs) are lipid bilayer-enclosed spheres that deliver RNA, protein, metabolite cargos donor recipient participate in many cellular processes. Emerging evidencehas shown microbial EVs play important roles cross-kingdom molecular exchange hosts microbes modulate host immunity pathogen virulence. Recent studies revealed function as a defense system by encasing delivering small RNAs (sRNAs) into pathogens, thereby mediating cross-species RNA interference silence virulence-related genes. This review focuses on latest advances our understanding transporting regulatory molecules, especially sRNAs, EV biogenesis also discussed, relies these

Language: Английский

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Bovine Milk-Derived Exosomes as a Drug Delivery Vehicle for miRNA-Based Therapy

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(3), P. 1105 - 1105

Published: Jan. 22, 2021

MicroRNAs (miRNAs) are small non-coding RNAs with a known role as mediators of gene expression in crucial biological processes, which converts them into high potential contenders the ongoing search for effective therapeutic strategies. However, extracellular unstable and rapidly degraded, reducing possibility successfully exerting function distant target cells. Strategies aimed at enhancing miRNAs include development efficient, tissue-specific nonimmunogenic delivery methods. Since were discovered to be naturally transported within exosomes, type vesicle that confers protection against RNase degradation increases miRNA stability have been proposed ideal vehicles miRNA-based therapy. Although research this field has grown last few years, standard, reproducible cost-effective protocol exosome isolation RNA is lacking. We evaluate use milk-derived vesicles drug delivery. With purpose, exosomes isolated from raw bovine milk, combining ultracentrifugation size exclusion chromatography (SEC) methodology. Isolated then loaded exogenous hsa-miR148a-3p, highly expressed milk exosomes. The suitability was tested by evaluating absorption miR-148a-3p hepatic (HepG2) intestinal (Caco-2) cell lines. exertion effect assessed analysis, using microarrays. Results support source can used nanocarriers functional RNA-based In addition, we show here combination SEC technics improve enrichment, purity, integrity subsequent use.

Language: Английский

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Engineering Exosome-Like Nanovesicles Derived from Asparagus cochinchinensis Can Inhibit the Proliferation of Hepatocellular Carcinoma Cells with Better Safety Profile

International Journal of Nanomedicine, Journal Year: 2021, Volume and Issue: Volume 16, P. 1575 - 1586

Published: Feb. 1, 2021

Background: Exosomes are a type of membrane vesicles secreted by living cells. Recent studies suggest exosome-like nanovesicles (ELNVs) from fruits and vegetables involved in tissue renewal process functional regulation against inflammatory diseases or cancers. However, there few reports on ELNVs derived medicinal plants. Methods: Asparagus cochinchinensis (Lour.) Merr. (ACNVs) were isolated characterized. Cytotoxicity, antiproliferative apoptosis-inducing capacity ACNVs hepatoma carcinoma cell assessed. The endocytosis mechanism was evaluated Hep G2 cells the presence different inhibitors. In vivo distribution detected healthy tumor-bearing mice after scavenger receptors (SRs) blockade. PEG engineering achieved through optimizing pharmacokinetic profiles. antitumor activity toxicity xenograft model. Results: purified using differential centrifugation method accompanied sucrose gradient ultracentrifugation. optimized had an average size about 119 nm showed typical cup-shaped nanostructure containing lipids, proteins, RNAs. found to possess specific proliferation associated with pathway. could be internalized into tumor mainly via phagocytosis, but they quickly cleared once entering blood. Blocking SRs PEGylation decoration prolonged blood circulation time increased accumulation sites. results that PEGylated significantly inhibit growth without side effects. Conclusion: This study provides promising nano platform edible can used therapy negligible Keywords: exosome, nanovesicles, ,

Language: Английский

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Plant-Derived Extracellular Vesicles as Therapeutic Nanocarriers

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 23(1), P. 191 - 191

Published: Dec. 24, 2021

Mammalian exosomes have emerged as a promising class of functional materials, inspiring novel applications therapeutic vehicles and nutraceutical compounds. Despite this, their immunogenicity has been an issue controversy within the scientific community. Although, exosome-like vesicles, innately formed in plants inherent to eukaryotic cell-derived could soothe most concerns, they are notably underutilized modalities. This review highlights all efforts published so far, on use plant-derived extracellular vesicles (EVs) delivery systems. A summary physicochemical characteristics EVs is provided along with main biological composition vitro/in vivo evidence efficacy where available. only hand full clinical trials being underway, concerning these arguably possess significant potential nanodelivery systems natural origin.

Language: Английский

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Plant Extracellular Vesicles and Nanovesicles: Focus on Secondary Metabolites, Proteins and Lipids with Perspectives on Their Potential and Sources

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(7), P. 3719 - 3719

Published: April 2, 2021

While human extracellular vesicles (EVs) have attracted a big deal of interest and been extensively characterized over the last years, plant-derived EVs nanovesicles earned less attention remained poorly investigated. Although series investigations already revealed promising beneficial health effects drug delivery properties, adequate (pre)clinical studies are rare. This fact might be caused by lack sources with appropriate qualities. Our study introduces plant cell suspension culture as new well controllable source for EVs. Plant cells, cultured in vitro, release into growth medium which could harvested pharmaceutical applications. In this investigation we from distinct sources. findings regarding secondary metabolites indicate that these not packaged an active manner but enriched membrane when lipophilic enough, since apparently compounds were associated while more hydrophilic structures consistently found. addition, protein identification possible explanation mechanism EV wall passage plants, hydrolases like 1,3-β-glucosidases, pectinesterases, polygalacturonases, β-galactosidases β-xylosidase/α-L-arabinofuranosidase 2-like present facilitate transition. Further on, identified proteins cells secrete using similar mechanisms animal to exosomes microvesicles.

Language: Английский

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Yam-derived exosome-like nanovesicles stimulate osteoblast formation and prevent osteoporosis in mice

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 355, P. 184 - 198

Published: Feb. 6, 2023

Language: Английский

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Characterization of the MicroRNA Profile of Ginger Exosome-like Nanoparticles and Their Anti-Inflammatory Effects in Intestinal Caco-2 Cells

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(15), P. 4725 - 4734

Published: March 9, 2022

Plant-derived exosome-like nanoparticles (PELNs) have been shown to enter mammalian cells for disease treatment. Although abundant miRNAs are contained in ginger (GELNs), little is known about their type and function. Herein, we extracted GELNs with desirable particle sizes (156 ± 36 nm) a negative surface charge (−26.6 5 mV). The miRNA profiles were analyzed using high-throughput sequencing, the results of sequencing validated by real-time quantitative polymerase chain reaction (RT-qPCR). There 27 higher expression levels GELNs, they mainly involved regulation inflammatory cancer-related pathways. Furthermore, could be specifically internalized intestine via caveolin-mediated endocytosis micropinocytosis, as well counteract lipopolysaccharide (LPS)-induced inflammation downregulating NF-κβ, IL-6, IL-8, TNF-α expression. Importantly, positive effects further proved possibly related enriched GELNs. Overall, these indicated that PELNs target human digestive organs play cross-kingdom physiological role through miRNAs.

Language: Английский

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