Transcriptomic exploration of the Coleopteran wings reveals insight into the evolution of novel structures associated with the beetle elytron DOI Creative Commons

David M. Linz,

Yuichiro Hara, Kevin D. Deem

et al.

Journal of Experimental Zoology Part B Molecular and Developmental Evolution, Journal Year: 2023, Volume and Issue: 340(2), P. 197 - 213

Published: Jan. 8, 2023

Abstract The acquisition of novel traits is central to organismal evolution, yet the molecular mechanisms underlying this process are elusive. beetle forewings (elytra) evolutionarily modified serve as a protective shield, providing unique opportunity study these mechanisms. In past, orthologs genes within wing gene network from Drosophila studies served starting point when studying evolution elytra (candidate genes). Although effective, candidate lists finite and only explore conserved across species. To go beyond genes, we used RNA sequencing explored transcriptomes two Coleopteran species, red flour ( Tribolium castaneum ) Japanese stag Dorcus hopei ). Our analysis revealed sets enriched in (57 genes) hindwings, which possess more “typical” insect morphologies (29 Over third hindwing‐enriched were “candidate genes” whose functions previously analyzed , demonstrating robustness our sequencing. overlap was limited, transcriptomic comparison between species found common set including key either or hindwings. interference for elytron‐enriched uncovered with roles forming various aspects morphology that elytra, such pigmentation, hardening, sensory development, vein formation. analyses deepen understanding how facilitated emergence elytron, structure critical evolutionary success beetles.

Language: Английский

The transition to flying insects: lessons from evo-devo and fossils DOI Creative Commons
Takahiro Ohde, Jakub Prokop

Current Opinion in Insect Science, Journal Year: 2025, Volume and Issue: unknown, P. 101332 - 101332

Published: Jan. 1, 2025

Insects are the only arthropod group to achieve powered flight, which facilitated their explosive radiation on land. It remains a significant challenge understand evolutionary transition from non-flying (apterygote) flying (pterygote) insects due large gap in fossil record. Under such situation, ontogenic information has historically been used compensate evidence. Recent evo-devo studies support and refine paleontology-based classical hypothesis that an ancestral exite incorporated into body wall contributed origin of insect wings. The modern locates precoxa leg segment with within hexapod lateral tergum, reframing long-standing debate wing origin. A current focus is contributions homolog surrounding tissues, as pleuron medial bona fide evolution. In parallel, recent analyses Paleozoic fossils have confirmed thoracic abdominal outgrowths transitional precursors, suggest possible role respiratory organs aquatic or semiaquatic environments. These revised our understanding insects. This review highlights progress both paleontology, discusses future challenges, including evolution metamorphic development.

Language: Английский

Citations

3
Activation of the Drosophila innate immune system accelerates growth in cooperation with oncogenic Ras DOI Creative Commons
Fabienne Brutscher, Federico Germani, George Hausmann

et al.

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(4), P. e3003068 - e3003068

Published: April 28, 2025

Innate immunity in Drosophila acts as an organismal surveillance system for external stimuli or cellular fitness and triggers context-specific responses to fight infections maintain tissue homeostasis. However, uncontrolled activation of innate immune pathways can be detrimental. In mammals, signaling is often overactivated malignant cells contributes tumor progression. models have been instrumental the discovery interactions between that promote tumorigenesis, but little known about whether how Toll pathway interacts with oncogenes. Here we use a epithelial vivo model investigate interplay oncogenic Ras. absence Ras (Ras V12 ), suppresses differentiation induces apoptosis. contrast, context , are protected from cell death Dorsal promotes survival proliferation drive hyperplasia. Taken together, show tissue-protective functions activity hijacked by pre-malignant induce tumorous overgrowth.

Language: Английский

Citations

1
Drosophila SPARC Collagen IV chaperone-like activity essential for development is unique to the fat body DOI Creative Commons

Samuel Delage,

Arya Zadhoosh,

Weon‐Kyoo You

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(4), P. 112111 - 112111

Published: Feb. 27, 2025

Drosophila fat body-derived SPARC acts as a chaperone for collagen IV (Col(IV)), enabling their diffusion and incorporation into distal tissue basement membranes (BMs). Disruption of or Col(IV) production by the body is lethal, despite expression other tissues such imaginal discs. Wing disc-derived does not associate with in BMs essential survival. We show that differential association body- wing due to differences glycosylation nor absence co-expression. Further, we demonstrate domain II/III produced sufficient both proximal BMs, rescues lethality associated loss SPARC. However, diffuse beyond hemolymph. Thus, chaperone-like activity specific required

Language: Английский

Citations

1
Shared enhancer gene regulatory networks between wound and oncogenic programs DOI Creative Commons
Swann Floc’hlay,

Ramya Balaji,

Dimitrije Stanković

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: May 3, 2023

Wound response programs are often activated during neoplastic growth in tumors. In both wound repair and tumor growth, cells respond to acute stress balance the activation of multiple programs, including apoptosis, proliferation, cell migration. Central those responses JNK/MAPK JAK/STAT signaling pathways. Yet, what extent these cascades interact at cis-regulatory level how they orchestrate different regulatory phenotypic is still unclear. Here, we aim characterize states that emerge cooperate response, using Drosophila melanogaster wing disc as a model system, compare with cancer induced by rasV12scrib-/- eye disc. We used single-cell multiome profiling derive enhancer gene networks (eGRNs) integrating chromatin accessibility expression signals. identify 'proliferative' eGRN, active majority wounded controlled AP-1 STAT. smaller, but distinct population cells, 'senescent' eGRN driven C/EBP-like transcription factors (Irbp18, Xrp1, Slow border, Vrille) Scalloped. These two signatures found be levels. Our eGRNs resource offers an in-depth characterization senescence markers, together new perspective on shared acting oncogenesis.

Language: Английский

Citations

17
Patterned apoptosis has an instructive role for local growth and tissue shape regulation in a fast-growing epithelium DOI Creative Commons
Alexis Matamoro‐Vidal, T. Cumming, Anđela Davidović

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(2), P. 376 - 388.e7

Published: Jan. 1, 2024

What regulates organ size and shape remains one fundamental mystery of modern biology. Research in this area has primarily focused on deciphering the regulation time space growth cell division, while contribution death been overall neglected. This includes studies Drosophila wing, best-characterized systems for study patterning, undergoing massive during larval stage important morphogenetic remodeling pupal stage. So far, it assumed that was relatively neglectable tissue both stage, as a result, pattern usually attributed to distribution division. Here, using systematic mapping registration combined with quantitative assessment clone disappearance well live imaging, we outline persistent elimination emerging wing disc persisting morphogenesis. Local variation is associated local size, pointing an impact not fully compensated by proliferation. Using morphometric analyses adult genetic perturbations, provide evidence patterned locally globally affects size. describes roadmap precise outlines instructive role modulating quantitatively morphogenesis fast-growing tissue.

Language: Английский

Citations

6
Assembly, dynamics and remodeling of epithelial cell junctions throughout development DOI
Marta Mira-Osuna, Roland Le Borgne

Development, Journal Year: 2024, Volume and Issue: 151(1)

Published: Jan. 1, 2024

ABSTRACT Cell junctions play key roles in epithelial integrity. During development, when epithelia undergo extensive morphogenesis, these must be remodeled order to maintain mechanochemical barriers and ensure the cohesion of tissue. In this Review, we present a comprehensive integrated description junctional remodeling mechanisms cells during from embryonic adult epithelia. We largely focus on Drosophila, as quantitative analyses organism have provided detailed characterization molecular governing cell topologies, discuss conservation across metazoans. consider how changes at level translate tissue-scale irreversible deformations, exploring composition assembly cellular interfaces unveil are preserve tissue homeostasis division, intercalation, invagination, ingression extrusion.

Language: Английский

Citations

6
Balancing competing effects of tissue growth and cytoskeletal regulation during Drosophila wing disc development DOI Creative Commons

Nilay Kumar,

Jennifer Rangel Ambriz,

Kevin Tsai

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 20, 2024

Abstract How a developing organ robustly coordinates the cellular mechanics and growth to reach final size shape remains poorly understood. Through iterations between experiments model simulations that include mechanistic description of interkinetic nuclear migration, we show local curvature, height, positioning cells in Drosophila wing imaginal disc are defined by concurrent patterning actomyosin contractility, cell-ECM adhesion, ECM stiffness, interfacial membrane tension. We increasing cell proliferation via different growth-promoting pathways results two distinct phenotypes. Triggering through insulin signaling increases basal but an increase Dpp Myc causes tissue flattening. These phenotypic outcomes arise from differences how each pathway regulates cytoskeleton, including contractility adhesion. The coupled regulation cytoskeletal regulators is general strategy meet multiple context-dependent criteria defining morphogenesis.

Language: Английский

Citations

6
Basal spot junctions of Drosophila epithelial tissues respond to morphogenetic forces and regulate Hippo signaling DOI Creative Commons
Benjamin Kroeger, Samuel A. Manning,

Yoshana Fonseka

et al.

Developmental Cell, Journal Year: 2023, Volume and Issue: 59(2), P. 262 - 279.e6

Published: Dec. 21, 2023

Language: Английский

Citations

12
Programmed disassembly of a microtubule-based membrane protrusion network coordinates 3D epithelial morphogenesis in Drosophila DOI Creative Commons
Ngan Vi Tran, Martti P. Montanari, Jinghua Gui

et al.

The EMBO Journal, Journal Year: 2024, Volume and Issue: 43(4), P. 568 - 594

Published: Jan. 23, 2024

Abstract Comprehensive analysis of cellular dynamics during the process morphogenesis is fundamental to understanding principles animal development. Despite recent advancements in light microscopy, how successive cell shape changes lead complex three-dimensional tissue still largely unresolved. Using vivo live imaging Drosophila wing development, we have studied unique structures comprising a microtubule-based membrane protrusion network. This network, which name here Interplanar Amida Network (IPAN), links two epithelium leaflets. Initially, IPAN sustains cell–cell contacts between layers through basal protrusions. Subsequent disassembly involves loss these contacts, with concomitant degeneration aligned microtubules. These processes are both autonomously and non-autonomously required for mitosis, leading coordinated proliferation epithelia. Our findings further reveal that microtubule organization switch from non-centrosomal centrosomal microtubule-organizing centers (MTOCs) at G2/M transition leads microtubule-derived exemplify change-mediated inter-tissue results 3D morphogenesis.

Language: Английский

Citations

5
Cell polarity and extrusion: How to polarize extrusion and extrude misspolarized cells? DOI
Ralitza Staneva, Romain Levayer

Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2023, Volume and Issue: unknown, P. 131 - 167

Published: Jan. 1, 2023

Language: Английский

Citations

11