Journal of Experimental Zoology Part B Molecular and Developmental Evolution,
Journal Year:
2023,
Volume and Issue:
340(2), P. 197 - 213
Published: Jan. 8, 2023
Abstract
The
acquisition
of
novel
traits
is
central
to
organismal
evolution,
yet
the
molecular
mechanisms
underlying
this
process
are
elusive.
beetle
forewings
(elytra)
evolutionarily
modified
serve
as
a
protective
shield,
providing
unique
opportunity
study
these
mechanisms.
In
past,
orthologs
genes
within
wing
gene
network
from
Drosophila
studies
served
starting
point
when
studying
evolution
elytra
(candidate
genes).
Although
effective,
candidate
lists
finite
and
only
explore
conserved
across
species.
To
go
beyond
genes,
we
used
RNA
sequencing
explored
transcriptomes
two
Coleopteran
species,
red
flour
(
Tribolium
castaneum
)
Japanese
stag
Dorcus
hopei
).
Our
analysis
revealed
sets
enriched
in
(57
genes)
hindwings,
which
possess
more
“typical”
insect
morphologies
(29
Over
third
hindwing‐enriched
were
“candidate
genes”
whose
functions
previously
analyzed
,
demonstrating
robustness
our
sequencing.
overlap
was
limited,
transcriptomic
comparison
between
species
found
common
set
including
key
either
or
hindwings.
interference
for
elytron‐enriched
uncovered
with
roles
forming
various
aspects
morphology
that
elytra,
such
pigmentation,
hardening,
sensory
development,
vein
formation.
analyses
deepen
understanding
how
facilitated
emergence
elytron,
structure
critical
evolutionary
success
beetles.
Current Opinion in Insect Science,
Journal Year:
2025,
Volume and Issue:
unknown, P. 101332 - 101332
Published: Jan. 1, 2025
Insects
are
the
only
arthropod
group
to
achieve
powered
flight,
which
facilitated
their
explosive
radiation
on
land.
It
remains
a
significant
challenge
understand
evolutionary
transition
from
non-flying
(apterygote)
flying
(pterygote)
insects
due
large
gap
in
fossil
record.
Under
such
situation,
ontogenic
information
has
historically
been
used
compensate
evidence.
Recent
evo-devo
studies
support
and
refine
paleontology-based
classical
hypothesis
that
an
ancestral
exite
incorporated
into
body
wall
contributed
origin
of
insect
wings.
The
modern
locates
precoxa
leg
segment
with
within
hexapod
lateral
tergum,
reframing
long-standing
debate
wing
origin.
A
current
focus
is
contributions
homolog
surrounding
tissues,
as
pleuron
medial
bona
fide
evolution.
In
parallel,
recent
analyses
Paleozoic
fossils
have
confirmed
thoracic
abdominal
outgrowths
transitional
precursors,
suggest
possible
role
respiratory
organs
aquatic
or
semiaquatic
environments.
These
revised
our
understanding
insects.
This
review
highlights
progress
both
paleontology,
discusses
future
challenges,
including
evolution
metamorphic
development.
PLoS Biology,
Journal Year:
2025,
Volume and Issue:
23(4), P. e3003068 - e3003068
Published: April 28, 2025
Innate
immunity
in
Drosophila
acts
as
an
organismal
surveillance
system
for
external
stimuli
or
cellular
fitness
and
triggers
context-specific
responses
to
fight
infections
maintain
tissue
homeostasis.
However,
uncontrolled
activation
of
innate
immune
pathways
can
be
detrimental.
In
mammals,
signaling
is
often
overactivated
malignant
cells
contributes
tumor
progression.
models
have
been
instrumental
the
discovery
interactions
between
that
promote
tumorigenesis,
but
little
known
about
whether
how
Toll
pathway
interacts
with
oncogenes.
Here
we
use
a
epithelial
vivo
model
investigate
interplay
oncogenic
Ras.
absence
Ras
(Ras
V12
),
suppresses
differentiation
induces
apoptosis.
contrast,
context
,
are
protected
from
cell
death
Dorsal
promotes
survival
proliferation
drive
hyperplasia.
Taken
together,
show
tissue-protective
functions
activity
hijacked
by
pre-malignant
induce
tumorous
overgrowth.
iScience,
Journal Year:
2025,
Volume and Issue:
28(4), P. 112111 - 112111
Published: Feb. 27, 2025
Drosophila
fat
body-derived
SPARC
acts
as
a
chaperone
for
collagen
IV
(Col(IV)),
enabling
their
diffusion
and
incorporation
into
distal
tissue
basement
membranes
(BMs).
Disruption
of
or
Col(IV)
production
by
the
body
is
lethal,
despite
expression
other
tissues
such
imaginal
discs.
Wing
disc-derived
does
not
associate
with
in
BMs
essential
survival.
We
show
that
differential
association
body-
wing
due
to
differences
glycosylation
nor
absence
co-expression.
Further,
we
demonstrate
domain
II/III
produced
sufficient
both
proximal
BMs,
rescues
lethality
associated
loss
SPARC.
However,
diffuse
beyond
hemolymph.
Thus,
chaperone-like
activity
specific
required
Wound
response
programs
are
often
activated
during
neoplastic
growth
in
tumors.
In
both
wound
repair
and
tumor
growth,
cells
respond
to
acute
stress
balance
the
activation
of
multiple
programs,
including
apoptosis,
proliferation,
cell
migration.
Central
those
responses
JNK/MAPK
JAK/STAT
signaling
pathways.
Yet,
what
extent
these
cascades
interact
at
cis-regulatory
level
how
they
orchestrate
different
regulatory
phenotypic
is
still
unclear.
Here,
we
aim
characterize
states
that
emerge
cooperate
response,
using
Drosophila
melanogaster
wing
disc
as
a
model
system,
compare
with
cancer
induced
by
rasV12scrib-/-
eye
disc.
We
used
single-cell
multiome
profiling
derive
enhancer
gene
networks
(eGRNs)
integrating
chromatin
accessibility
expression
signals.
identify
'proliferative'
eGRN,
active
majority
wounded
controlled
AP-1
STAT.
smaller,
but
distinct
population
cells,
'senescent'
eGRN
driven
C/EBP-like
transcription
factors
(Irbp18,
Xrp1,
Slow
border,
Vrille)
Scalloped.
These
two
signatures
found
be
levels.
Our
eGRNs
resource
offers
an
in-depth
characterization
senescence
markers,
together
new
perspective
on
shared
acting
oncogenesis.
Current Biology,
Journal Year:
2024,
Volume and Issue:
34(2), P. 376 - 388.e7
Published: Jan. 1, 2024
What
regulates
organ
size
and
shape
remains
one
fundamental
mystery
of
modern
biology.
Research
in
this
area
has
primarily
focused
on
deciphering
the
regulation
time
space
growth
cell
division,
while
contribution
death
been
overall
neglected.
This
includes
studies
Drosophila
wing,
best-characterized
systems
for
study
patterning,
undergoing
massive
during
larval
stage
important
morphogenetic
remodeling
pupal
stage.
So
far,
it
assumed
that
was
relatively
neglectable
tissue
both
stage,
as
a
result,
pattern
usually
attributed
to
distribution
division.
Here,
using
systematic
mapping
registration
combined
with
quantitative
assessment
clone
disappearance
well
live
imaging,
we
outline
persistent
elimination
emerging
wing
disc
persisting
morphogenesis.
Local
variation
is
associated
local
size,
pointing
an
impact
not
fully
compensated
by
proliferation.
Using
morphometric
analyses
adult
genetic
perturbations,
provide
evidence
patterned
locally
globally
affects
size.
describes
roadmap
precise
outlines
instructive
role
modulating
quantitatively
morphogenesis
fast-growing
tissue.
Development,
Journal Year:
2024,
Volume and Issue:
151(1)
Published: Jan. 1, 2024
ABSTRACT
Cell
junctions
play
key
roles
in
epithelial
integrity.
During
development,
when
epithelia
undergo
extensive
morphogenesis,
these
must
be
remodeled
order
to
maintain
mechanochemical
barriers
and
ensure
the
cohesion
of
tissue.
In
this
Review,
we
present
a
comprehensive
integrated
description
junctional
remodeling
mechanisms
cells
during
from
embryonic
adult
epithelia.
We
largely
focus
on
Drosophila,
as
quantitative
analyses
organism
have
provided
detailed
characterization
molecular
governing
cell
topologies,
discuss
conservation
across
metazoans.
consider
how
changes
at
level
translate
tissue-scale
irreversible
deformations,
exploring
composition
assembly
cellular
interfaces
unveil
are
preserve
tissue
homeostasis
division,
intercalation,
invagination,
ingression
extrusion.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: March 20, 2024
Abstract
How
a
developing
organ
robustly
coordinates
the
cellular
mechanics
and
growth
to
reach
final
size
shape
remains
poorly
understood.
Through
iterations
between
experiments
model
simulations
that
include
mechanistic
description
of
interkinetic
nuclear
migration,
we
show
local
curvature,
height,
positioning
cells
in
Drosophila
wing
imaginal
disc
are
defined
by
concurrent
patterning
actomyosin
contractility,
cell-ECM
adhesion,
ECM
stiffness,
interfacial
membrane
tension.
We
increasing
cell
proliferation
via
different
growth-promoting
pathways
results
two
distinct
phenotypes.
Triggering
through
insulin
signaling
increases
basal
but
an
increase
Dpp
Myc
causes
tissue
flattening.
These
phenotypic
outcomes
arise
from
differences
how
each
pathway
regulates
cytoskeleton,
including
contractility
adhesion.
The
coupled
regulation
cytoskeletal
regulators
is
general
strategy
meet
multiple
context-dependent
criteria
defining
morphogenesis.
The EMBO Journal,
Journal Year:
2024,
Volume and Issue:
43(4), P. 568 - 594
Published: Jan. 23, 2024
Abstract
Comprehensive
analysis
of
cellular
dynamics
during
the
process
morphogenesis
is
fundamental
to
understanding
principles
animal
development.
Despite
recent
advancements
in
light
microscopy,
how
successive
cell
shape
changes
lead
complex
three-dimensional
tissue
still
largely
unresolved.
Using
vivo
live
imaging
Drosophila
wing
development,
we
have
studied
unique
structures
comprising
a
microtubule-based
membrane
protrusion
network.
This
network,
which
name
here
Interplanar
Amida
Network
(IPAN),
links
two
epithelium
leaflets.
Initially,
IPAN
sustains
cell–cell
contacts
between
layers
through
basal
protrusions.
Subsequent
disassembly
involves
loss
these
contacts,
with
concomitant
degeneration
aligned
microtubules.
These
processes
are
both
autonomously
and
non-autonomously
required
for
mitosis,
leading
coordinated
proliferation
epithelia.
Our
findings
further
reveal
that
microtubule
organization
switch
from
non-centrosomal
centrosomal
microtubule-organizing
centers
(MTOCs)
at
G2/M
transition
leads
microtubule-derived
exemplify
change-mediated
inter-tissue
results
3D
morphogenesis.