Mechanistic insights into DNA damage recognition and checkpoint control in plants
Nature Plants,
Journal Year:
2024,
Volume and Issue:
10(4), P. 539 - 550
Published: March 19, 2024
Language: Английский
Long non-coding RNA-mediated epigenetic response for abiotic stress tolerance in plants
Plant Physiology and Biochemistry,
Journal Year:
2023,
Volume and Issue:
206, P. 108165 - 108165
Published: Nov. 9, 2023
Language: Английский
Comet Assay: Multifaceted Options for Studies of Plant Stress Response
Horticulturae,
Journal Year:
2024,
Volume and Issue:
10(2), P. 174 - 174
Published: Feb. 15, 2024
Contrarily
to
chronic
stresses,
acute
(i.e.,
fast
and
dramatic)
changes
in
environmental
factors
like
temperature,
radiation,
concentration
of
toxic
substances,
or
pathogen
attack
often
lead
DNA
damage.
Some
the
stress
are
genotoxic,
i.e.,
they
damage
via
physical
interactions
interference
with
replication/repair
machinery.
However,
cytotoxic
factors,
those
that
do
not
directly
DNA,
can
secondary
genotoxic
effects
either
induction
production
reactive
oxygen,
carbon,
nitrogen
species,
activation
programmed
cell
death
related
endonucleases.
The
extent
this
damage,
as
well
ability
repair
it,
represent
a
significant
part
plant
responses.
Information
about
is
important
for
physiological
studies
it
helps
understand
complex
adaptive
responses
plants
even
predict
outcome
plant’s
exposure
stress.
Single
gel
electrophoresis
(Comet
assay)
provides
convenient
relatively
inexpensive
tool
evaluate
strand
breaks
different
organs
higher
plants,
unicellular
algae.
Comet
assays
widely
used
ecotoxicology
biomonitoring
applications;
however,
still
rarely
studies.
In
review,
we
provide
an
overview
basic
principles
useful
variations
protocols
assays,
their
use
studies,
order
encourage
physiologists
include
analysis
Language: Английский
Chromatin dynamics and RNA metabolism are double-edged swords for the maintenance of plant genome integrity
Nature Plants,
Journal Year:
2024,
Volume and Issue:
10(6), P. 857 - 873
Published: April 24, 2024
Language: Английский
The long non‐coding RNALINDA restrains cellular collapse following DNA damage in Arabidopsis thaliana
The Plant Journal,
Journal Year:
2023,
Volume and Issue:
116(5), P. 1370 - 1384
Published: Aug. 24, 2023
SUMMARY
The
genomic
integrity
of
every
organism
is
endangered
by
various
intrinsic
and
extrinsic
stresses.
To
maintain
integrity,
a
sophisticated
DNA
damage
response
(DDR)
network
activated
rapidly
after
damage.
Notably,
the
fundamental
DDR
mechanisms
are
conserved
in
eukaryotes.
However,
knowledge
about
many
regulatory
aspects
plant
still
limited.
Important,
yet
little
understood,
factors
long
non‐coding
RNAs
(lncRNAs).
In
humans,
13
lncRNAs
functioning
have
been
characterized
to
date,
whereas
no
such
plants
yet.
By
meta‐analysis,
we
identified
putative
intergenic
RNA
induced
(
LINDA
)
that
responds
strongly
double‐strand
break‐inducing
treatments,
but
not
replication
stress
mitomycin
C.
After
damage,
an
ATM‐
SOG1‐dependent
manner.
Intriguingly,
transcriptional
similar
its
flanking
hypothetical
protein‐encoding
gene.
Phylogenetic
analysis
Brassicales
Malvales
homologs
indicates
originate
from
duplication
small
gene
followed
pseudogenization.
We
demonstrate
only
needed
for
regulation
this
also
fine‐tuning
occurrence
breaks.
Moreover,
Δlinda
mutant
root
stem
cells
unable
recover
most
likely
due
hyper‐induced
cell
death.
Language: Английский
SPPiDDRs: a new gene family in dicot plants involved in DNA-Damage Response
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 21, 2023
ABSTRACT
Living
organisms
must
maintain
the
integrity
of
their
genome,
and
plants
are
not
exempt.
In
plants,
recognition
DNA
damage
converges
at
transcription
factor
SOG1,
a
functional
homolog
animal
p53
protein.
SOG1
directly
controls
expression
hundreds
genes
orchestrates
sophisticated
network
signaling
pathways
termed
DNA-damage
response
(DDR).
Only
recently,
several
long
non-coding
RNA
(lncRNA)
loci
were
identified
to
be
upregulated
by
damage,
only
handful
have
been
confirmed
actively
contribute
DDR.
this
study,
we
focused
on
one
locus
annotated
as
lncRNA
found
that
it
is
strongly
quickly
upon
direct
target
SOG1.
Combining
in
silico
experimental
analyses,
demonstrate
was
wrongly
fact
gene
coding
for
short
protein
targets
peroxisomes.
Consequently,
renamed
S
HORT
P
EROXISOMAL
ROTEIN
INDUCED
IN
D
NA-
AMAGE
R
ESPONSE1
(
SPPiDDR1
).
SPPiDDRs
well
conserved
present
multiple
copies
across
dicot
genomes,
with
Arabidopsis
containing
two
additional
copies,
SPPiDDR2
SPPiDDR3
.
The
AtSPPiDDR
paralogs
differ
transcriptional
level,
being
least
active.
both
also
induced
salt,
stress
treatment
known
indirectly
induce
via
oxidative
stress.
We
show
these
act
redundantly
inhibit
plant
growth
salt
Language: Английский