CRISPR/Cas基因组编辑在木本植物性状改良中的应用 DOI

Xuening Yuan,

Fengge Yao,

Yi An

et al.

Chinese Science Bulletin (Chinese Version), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 1, 2023

Development of a xylem protoplast expression system for gene function analysis and genome editing in Fraxinus mandshurica Rupr DOI Creative Commons
Lu Han, Zhang Hong, Pengfei Wang

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 225, P. 120446 - 120446

Published: Jan. 8, 2025

Language: Английский

Citations

0
Enhancement of Dendrobine Production by CRISPR/Act3.0-Mediated Transcriptional Activation of Multiple Endogenous Genes in Dendrobium Plants DOI Open Access
Meili Zhao,

Zhenyu Yang,

Jian Li

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1487 - 1487

Published: Feb. 11, 2025

Dendrobine, a significant medicinal compound, typically accumulates at low concentrations within several Dendrobium species, including nobile, catenatum, and moniliforme. As D. nobile catenatum are established ingredients in traditional Chinese medicine have been cultivated extensively, they present ideal plant chassis for upscaling dendrobine production industrial research applications. This study employed two approaches: the ectopic overexpression of seven genes through multigene stacking activation multiple key endogenous synthesis pathway using CRISPR/Act3.0 either or catenatum. These methods enhanced transiently infiltrated leaves by 30.1% transgenic plants 35.6%. The is first to apply orchids, enhancing production, thus laying solid foundation further improvements. recently developed technique that demonstrates high efficiency model rice, maize, Arabidopsis. By combining CRISPR with transcriptional regulatory modules, metabolic can be achieved. successful application orchid molecular breeding reveals promising potential exploration.

Language: Английский

Citations

0
Advancing plant gene activation with CRISPR-Act3.0 DOI
Ali Movahedi,

Zhiying Mu,

Liming Yang

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
CRISPR plants for studying functional genomics: Methods and applications DOI
Mireia Uranga, Ana Montserrat Martín‐Hernández, Fabio Pasin

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 27 - 59

Published: Jan. 1, 2025

Language: Английский

Citations

0
Metabolic changes and potential biomarkers in different cultivars of black poplar: aiding in the identification of poplar cultivars DOI

Yuhang Deng,

Boxiao Wu,

Jiandong Tang

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109891 - 109891

Published: April 1, 2025

Language: Английский

Citations

0
Forest Tree Breeding Under the Global Environmental Change: Challenges and Opportunities DOI Creative Commons
Chenglin Li,

B.B. Li,

Wenxuan Zhao

et al.

Trees Forests and People, Journal Year: 2025, Volume and Issue: unknown, P. 100867 - 100867

Published: April 1, 2025

Language: Английский

Citations

0
Strategies for improving the genome-editing efficiency of class 2 CRISPR/Cas system DOI Creative Commons
Linli Wang, Hongbing Han

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38588 - e38588

Published: Sept. 27, 2024

Language: Английский

Citations

2
Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens? DOI Open Access
Maxim A. Kovalev, Natalya S. Gladysh, Alina S. Bogdanova

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1308 - 1308

Published: Jan. 21, 2024

Poplar (Populus) is a genus of woody plants great economic value. Due to the growing importance poplar, there need ensure its stable growth by increasing resistance pathogens. Genetic engineering can create organisms with improved traits faster than traditional methods, and development CRISPR/Cas-based genome editing systems, scientists have new highly effective tool for creating valuable genotypes. In this review, we summarize latest research data on poplar diseases, biology their pathogens how these resist final section, propose plant male or mixed populations; consider genes MLO group, transcription factors WRKY MYB families defensive proteins BbChit1, LJAMP2, MsrA2 PtDef as most promising targets genetic engineering; also pay attention possibility microbiome engineering.

Language: Английский

Citations

1
Advancements of CRISPR-Mediated Base Editing in Crops and Potential Applications in Populus DOI Open Access
Xuefei Yang, Ping Zhu, Jinshan Gui

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8314 - 8314

Published: July 30, 2024

Base editing represents a cutting-edge genome technique that utilizes the CRISPR system to guide base deaminases with high precision specific genomic sites, facilitating targeted alteration of individual nucleotides. Unlike traditional gene approaches, does not require DNA double-strand breaks or donor templates. It functions independently cellular repair machinery, offering significant advantages in terms both efficiency and accuracy. In this review, we summarize core design principles various editors, their distinctive characteristics, tactics refine efficacy. We also applications crop genetic improvement explore potential contributions forest engineering.

Language: Английский

Citations

1
CRISPR/Cas9-mediated mutation of FERULATE 5-HYDROXYLASE enhances adsorption capacity of lignocellulose-based porous carbon from paper mulberry DOI Creative Commons

Yue Xu,

Yingping Cao, Wanju Zhang

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(6)

Published: April 23, 2024

Language: Английский

Citations

0