Horticulture Research,
Journal Year:
2024,
Volume and Issue:
11(6)
Published: April 16, 2024
The
economically
significant
genus
Prunus
includes
fruit
and
nut
crops
that
have
been
domesticated
for
shared
specific
agronomic
traits;
however,
the
genomic
signals
of
convergent
divergent
selection
not
elucidated.
In
this
study,
we
aimed
to
detect
signatures
by
conducting
comparative
population
analyses
apricot-peach-plum-mei
(APPM)
complex,
utilizing
a
haplotype-resolved
telomere-to-telomere
(T2T)
genome
assembly
resequencing
data.
T2T
reference
plum
cultivar
was
assembled
through
HiFi
Hi-C
reads,
resulting
in
two
haplotypes
251.25
251.29
Mb
size,
respectively.
Comparative
genomics
reveals
chromosomal
translocation
~1.17
apricot
genomes
compared
with
peach,
plum,
mei.
Notably,
involves
D
locus,
significantly
impacting
titratable
acidity
(TA),
pH,
sugar
content.
Population
genetic
analysis
detected
substantial
gene
flow
between
apricot,
introgression
regions
enriched
post-embryonic
development
pollen
germination
processes.
revealed
stress
tolerance,
flower
development,
ripening,
along
shaping
crop,
such
as
somatic
embryogenesis
mei,
hormone
regulation
peach.
selective
sweeps
on
chromosome
7
coincide
collinearity
from
genomics,
key
fruit-softening
genes
PG,
regulated
ERF
RMA1H1.
Overall,
study
provides
insights
into
diversity,
evolutionary
history,
domestication
APPM
offering
valuable
implications
studies
breeding
programs
crops.
Plant Communications,
Journal Year:
2024,
Volume and Issue:
5(9), P. 100983 - 100983
Published: June 5, 2024
Whole-genome
genotyping
(WGG)
stands
as
a
pivotal
element
in
genomic-assisted
plant
breeding.
Nevertheless,
sequencing-based
approaches
for
WGG
continue
to
be
costly,
primarily
owing
the
high
expenses
associated
with
library
preparation
and
laborious
protocol.
During
prior
development
of
foreground
background
integrated
by
sequencing
(FBI-seq),
we
discovered
that
any
sequence-specific
primer
(SP)
inherently
possesses
capability
amplify
massive
array
stable
reproducible
non-specific
PCR
products
across
genome.
Here,
further
improved
FBI-seq
replacing
adapter
ligated
Tn5
transposase
an
arbitrary
degenerate
(AD)
primer.
The
protocol
enhanced
unexpectedly
mirrors
simplified
thermal
asymmetric
interlaced
(TAIL)-PCR,
technique
is
widely
used
isolation
flanking
sequences.
However,
TAIL-PCR
maximizes
primer-template
mismatched
annealing
capabilities
both
SP
AD
primers.
In
addition,
leveraging
next-generation
enhances
ability
this
assay
tens
thousands
genome-wide
loci
species.
This
cost-effective,
user-friendly,
powerful
tool,
which
have
named
(TAIL-peq),
holds
great
potential
widespread
application
breeding
programs,
thereby
facilitating
genome-assisted
crop
improvement.
Plant Physiology and Biochemistry,
Journal Year:
2024,
Volume and Issue:
unknown, P. 109450 - 109450
Published: Dec. 1, 2024
The
ATP-binding
cassette
(ABC)
gene
family
comprises
some
of
the
most
critical
transporter
proteins
in
plants,
playing
vital
roles
maintaining
cellular
homeostasis
and
adapting
to
environmental
changes.
While
ABC
transporters
have
been
extensively
characterized
various
plant
species,
their
profile
C.
mollissima
remains
less
understood.
In
this
study,
164
genes
were
identified
within
genome,
subsequently
classified
into
eight
subfamilies.
Collinear
analysis
suggested
that
dispersed
duplication
was
primary
mechanism
driving
expansion
CmABC
family.
study
also
examined
morphological
physiological
changes
under
temperature
stress,
highlighting
significant
decreases
photosynthetic
indicators
SOD
enzyme
activity,
while
other
varied.
Transcriptome
revealed
distinct
expression
patterns
identifying
CmABCG29c
CmABCB11e
as
key
candidates
for
responding
stress.
This
based
on
patterns,
correlation
with
indicators,
WGCNA
analysis.
levels
measured
RT-qPCR
experiments
consistent
those
observed
RNA-seq
research
provides
a
theoretical
foundation
understanding
responses
Agronomy,
Journal Year:
2024,
Volume and Issue:
15(1), P. 58 - 58
Published: Dec. 28, 2024
Fruit
quality
is
one
of
the
most
important
economic
traits
fruit
crops,
directly
influencing
market
prices
and
orchard
revenues.
Enhancing
has
therefore
become
a
critical
objective
in
both
production
scientific
research.
External
fruits
typically
include
size,
coloration,
shape,
uniformity,
consistency.
Internal
refer
to
nutritional
value
fruits,
which
largely
determine
their
flavor
palatability.
Over
past
decade,
significant
progress
been
made
understanding
formation
regulatory
mechanisms
quality,
with
numerous
key
functional
genes
associated
being
identified.
While
substantial
advancements
have
achieved
studying
specific
aspects
there
remains
lack
comprehensive
systematic
reviews
addressing
overall
physiology
interplay
among
various
traits,
diversity
mechanisms.
Using
Chinese
pears
as
an
example,
this
review
summarizes
research
regulation
over
five
years.
Key
metabolic
such
sweetness,
color,
texture,
physiological
disorders;
factors
stone
cell
formation;
sugar
content
regulation;
roles
plant
hormones
including
ethylene,
gibberellins,
abscisic
acid;
translational
post-translational
modifications
ubiquitination,
methylation,
acetylation;
well
application
genomic
sequencing
technologies.
Furthermore,
offers
practical
suggestions
for
improving
pear
provides
insights
researchers
related
fields.
Finally,
future
trends
are
discussed,
offering
forward-looking
perspective
advancing
field.
Horticulturae,
Journal Year:
2024,
Volume and Issue:
10(12), P. 1305 - 1305
Published: Dec. 7, 2024
ALMT
(aluminum-activated
malate
transporter)
proteins
play
crucial
roles
in
the
transport
of
organic
acids
and
have
significant
implications
for
plant
stress
responses
development.
While
extensively
studied
some
plants,
characteristics
functional
divergence
gene
family
not
yet
been
thoroughly
explored
Prunus
mume
other
Rosaceae
species.
In
this
study,
we
systematically
analyzed
across
nine
species
to
explore
their
evolutionary
relationships,
structural
characteristics,
roles.
A
total
138
genes
were
identified
categorized
into
four
groups
based
on
a
phylogenetic
analysis.
The
motif
analysis
confirmed
accuracy
grouping.
collinearity
indicated
that
whole-genome
duplication
events
primary
drivers
expansion
these
Furthermore,
cis-acting
element
revealed
diverse
regulatory
elements
associated
with
environmental
responses,
including
abscisic
acid,
light,
jasmonic
acid.
expression
correlation
showed
PmALMT1
is
primarily
malic
acid
accumulation,
whereas
PmALMT8
related
citric
accumulation.
Further
transient
Nicotiana
benthamiana
validated
above
conclusion.
This
comprehensive
provides
valuable
insights
evolution,
function,
regulation
Horticulture Research,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 4, 2024
Brassica
rapa
includes
a
variety
of
important
vegetable
and
oilseed
crops,
yet
it
is
significantly
challenged
by
clubroot
disease.
Notably,
the
majority
genotypes
B.
with
published
genomes
exhibit
high
susceptibility
to
The
present
study
presents
high-quality
chromosome-level
sequence
genome
DH40
clubroot-resistant
(CR)
line,
doubled
haploid
line
derived
from
hybrid
progeny
European
turnip
(ECD01)
two
lines
Chinese
cabbage.
assembled
spans
420.92
Mb,
contig
N50
size
11.97
Mb.
Comparative
genomics
studies
revealed
that
more
closely
related
cabbage
Chiifu
than
ECD04.
provided
direct
reference
greatly
facilitate
map-based
cloning
resistance
gene
Crr5,
encoding
nucleotide-binding
leucine-rich
repeat
(NLR)
protein.
Further
functional
analysis
demonstrated
Crr5
confers
in
both
transgenic
Arabidopsis.
It
responds
inoculation
Plasmodiophora
brassicae
expressed
roots
leaves.
Subcellular
localization
shows
nucleus.
Toll/interleukin-1
receptor
(TIR)
domain
can
autoactivate
trigger
cell
death.
In
addition,
we
developed
Crr5-specific
Kompetitive
allele-specific
PCR
(KASP)
markers
showcased
their
successful
application
breeding
CR
through
marker-assisted
selection.
Overall,
our
research
offers
valuable
resources
for
genetic
genomic
deepens
understanding
molecular
mechanisms
underlying
against
P.
brassicae.
