Artificial miRNAs and target-mimics as potential tools for crop improvement
Physiology and Molecular Biology of Plants,
Journal Year:
2025,
Volume and Issue:
31(1), P. 67 - 91
Published: Jan. 1, 2025
Language: Английский
Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (Stagonosporopsis cucurbitacearum) of Melon Plant Introduction (PI) 420145
Horticulturae,
Journal Year:
2025,
Volume and Issue:
11(1), P. 31 - 31
Published: Jan. 2, 2025
Gummy
stem
blight
(GSB),
caused
by
Stagonosporopsis
cucurbitacearum
(Didymella
bryoniae),
poses
a
growing
threat
to
greenhouse
melon
production.
Despite
this,
the
defense
mechanism
of
against
S.
remains
poorly
understood.
In
this
study,
employing
electron
microscopy
and
transcriptome
sequencing,
we
investigated
cellular
ultrastructure
differences
gene
expression
dynamics
two
accessions,
PI
420145
(resistant
GSB)
‘Baipicui’
(susceptible),
pre-
post-
inoculation.
Our
results
revealed
that
exhibits
thicker
waxy
layer
on
leaf
surface
limited
conidial
germination
without
obvious
signs
cell
damage
compared
‘Baipicui’.
The
transcriptomic
analysis
identified
total
23,078
differentially
expressed
genes
(DEGs)
974
long
noncoding
RNAs
(DELs).
Specifically,
phenylpropanoid
biosynthesis,
cyanoamino
acid
metabolism,
MAPK
signaling–plant
plant–pathogen
interactions
were
enriched
in
420145,
while
displayed
enrichment
metabolism
autophagy.
Additionally,
through
lncRNA–mRNA
network
construction,
38
targeted
regulatory
relationships
four
most
significant
KEGG
pathways
for
disease
resistance.
Language: Английский
Transcriptional junk: Waste or a key regulator in diverse biological processes?
Anwesha Anyatama,
No information about this author
Tapasya Datta,
No information about this author
Shambhavi Dwivedi
No information about this author
et al.
Current Opinion in Plant Biology,
Journal Year:
2024,
Volume and Issue:
82, P. 102639 - 102639
Published: Sept. 26, 2024
Language: Английский
LTR retrotransposon-derived novel lncRNA2 enhances cold tolerance in Moso bamboo by modulating antioxidant activity and photosynthetic efficiency
PeerJ,
Journal Year:
2025,
Volume and Issue:
13, P. e19056 - e19056
Published: Feb. 26, 2025
In
Moso
bamboo,
the
mechanism
of
long
terminal
repeat
(LTR)
retrotransposon-derived
non-coding
RNA
(TElncRNA)
in
response
to
cold
stress
remains
unclear.
this
study,
several
Pe-TElncRNAs
were
identified
from
bamboo
transcriptome
data.
qRT-PCR
analysis
showed
that
expression
a
novel
Pe-TElncRNA2
seedlings
reached
its
highest
level
at
8
hours
treatment
4
°C
and
was
significantly
higher
stems
compared
leaves,
roots,
buds.
Furthermore,
cellular
localization
revealed
cytoplasm
than
nucleus.
overexpression
protoplasts
positively
regulated
FZR2
,
NOT3
ABCG44
AGD6
genes.
Further
validation
lncRNA
Arabidopsis
thaliana
enhanced
antioxidant
activities,
as
evidenced
by
increased
superoxide
dismutase
(SOD)
activity
proline
content,
well
maximum
photochemical
efficiency
PS
II
dark-adapted
leaves
(
F
v
/
m
),
transgenic
plants
wild-type
controls.
Conversely,
malondialdehyde
(MDA)
lipid
peroxidation
marker
(a
oxidative
stress),
reduced
plants.
Notably,
levels
both
genes
upregulated
after
two
days
treatment.
These
findings
elucidate
critical
role
LTR
lncRNAs
mediating
responses
bamboo.
Language: Английский
A translocation between chromosome 6 and 8 influences lncRNA_MYB114 and PpRPP13 expression and underpins red leaf trait and powdery mildew resistance in peach
New Phytologist,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 4, 2025
Summary
Red
leaf
peach
has
important
ornamental
value
owing
to
its
characteristic
coloration.
However,
this
species
is
highly
susceptible
powdery
mildew,
and
the
mechanisms
of
red
formation,
resistance
their
relationship
remain
unclear.
We
performed
population
genetic
analyses
peach,
revealing
that
translocation
chromosomes
6
8
genetically
linked
both
trait
mildew
resistance.
Bulk
segregant
analysis‐sequencing,
genome
resequencing,
expression
analysis
indicated
PpMYB114
gene
PpRPP13
are
responsible
for
phenotype
resistance,
respectively.
The
chromosomal
causes
a
promoter
fragment
on
chromosome
integrate
into
antisense
chain
6,
thereby
enhancing
inhibiting
.
Further,
lncRNA‐seq
identified
new
lncRNA,
lncRNA_MYB114
,
which
generated
by
can
activate
synthesize
anthocyanin.
Moreover,
overexpression
resulted
in
enhanced
mildew.
In
summary,
these
results
revealed
molecular
mechanism
altering
form
susceptibility.
Language: Английский
Multidimensional analysis of long noncoding RNAs function in Solanaceae plants
New Phytologist,
Journal Year:
2025,
Volume and Issue:
unknown
Published: June 1, 2025
Summary
Long
noncoding
RNAs
(lncRNAs)
have
been
gradually
verified
as
functional
regulators
in
plants,
yet
their
functions
remain
underexplored,
especially
the
Solanaceae
family.
While
substantial
progress
has
made
identifying
lncRNAs
different
species,
systematic
annotations
are
lacking.
In
this
study,
we
uniformly
identified
and
systematically
characterized
from
seven
Solanaceous
species
using
large‐scale
strand‐specific
RNA‐seq
(ssRNA‐seq).
About
113
700
lncRNA
genes
were
obtained
analyzed
for
sequence,
conservation,
expression
profile,
epigenetic
signals,
genetic
mutants.
tomato,
97.4%
of
annotated
with
basic
characteristics.
Also,
25.7%
further
predicted
to
be
involved
stress
response,
development,
metabolism.
Comparisons
between
protein‐coding
(PCGs)
highlighted
unique
characteristics
tissue
expression,
responses,
sequence
composition,
signal
distribution.
We
shared
our
datasets
on
at
http://solanaceae‐lncrna‐source.liu‐lab.com/
.
Taking
fruit
development
ripening
an
example,
mined
data
resources
a
total
1158
associated
process,
presenting
how
curated
can
utilized
discover
plant
lncRNAs.
Overall,
study
provides
comprehensive
multidimensional
framework
research,
which
serves
valuable
reference
understanding
other
plants.
Language: Английский
Comparative RNA-seq analysis of tomato (Solanum lycopersicum L.) provides insights into natural and postharvest ripening
Xiuming Zhao,
No information about this author
Fujun Li,
No information about this author
Jun Ding
No information about this author
et al.
Postharvest Biology and Technology,
Journal Year:
2024,
Volume and Issue:
216, P. 113079 - 113079
Published: July 1, 2024
Language: Английский
Metabolomic and Transcriptomic Analysis Revealed the Maturation Mechanism of White-Fleshed Strawberry
Lin-Lin Xu,
No information about this author
Xiaohong Chen,
No information about this author
Qinglian Wang
No information about this author
et al.
Agronomy,
Journal Year:
2024,
Volume and Issue:
14(12), P. 2860 - 2860
Published: Nov. 30, 2024
Strawberry
(Fragaria
ananassa)
is
a
widely
grown
horticultural
crop,
which
exists
in
red,
yellow,
and
white
varieties.
In
recent
years,
the
white-fleshed
strawberry
variety
gaining
more
attention
from
consumers
for
its
unique
taste
appearance,
but
comprehensive
understanding
of
molecular
processes
governing
ripening
remains
undisclosed.
this
study,
based
on
joint
analysis
physiology,
metabolome,
transcriptome,
we
screened
identified
key
metabolites
that
were
highly
correlated
to
maturation
(cv.
‘snow
white’,
SW
short)
fruits.
contrast
red-fleshed
strawberries,
fruits
exhibited
three
main
stages
during
maturation,
accompanied
by
increases
total
soluble
solid
sugar
declines
anthocyanin
acid.
Metabolomic
832
differential
accumulated
(DAMs)
at
secondary
level
LC-MS/MS,
further
investigations
suggested
increase
sucrose,
citric
acid,
epicatechin
levels
potentially
play
role
process
Furthermore,
abscisic
acid
methyl
jasmonate
recognized
as
primary
phytohormones
involved
production
these
metabolites.
The
enrichment
RNA-Seq
data
revealed
expressed
genes
(DEGs)
primarily
attributed
pathways
‘Starch
sucrose
metabolism’
‘Plant
hormone
signal
transduction’
undetected
‘Flavonoid
biosynthesis’
late
stage.
Moreover,
de
novo
biosynthesis
pathway,
WGCNA,
Pearson
correlation
indicated
direct
relationship
between
FaSPS1,
FaSPP1,
FaSPP2
with
FaPEPC1,
FaV-PPase2,
FaV-PPase3
Fa4CL2,
Fa4CL3,
FaANR1
anthocyanin.
Further
co-expression
MYBs,
bHLHs,
NACs,
WRKYs
structural
mentioned.
Overall,
our
findings
uncovered
mechanism
regulating
strawberry,
providing
valuable
insights
enhancing
flavor
through
gene-editing
technique.
Language: Английский
Long non-coding RNAs: a promising tool to improve horticultural quality traits
Current Plant Biology,
Journal Year:
2024,
Volume and Issue:
40, P. 100413 - 100413
Published: Nov. 6, 2024
Language: Английский
Localization is the key to action: regulatory peculiarities of lncRNAs
Frontiers in Genetics,
Journal Year:
2024,
Volume and Issue:
15
Published: Dec. 16, 2024
To
understand
the
transcriptomic
profile
of
an
individual
cell
in
a
multicellular
organism,
we
must
comprehend
its
surrounding
environment
and
cellular
space
where
distinct
molecular
stimuli
responses
are
located.
Contradicting
initial
perception
that
RNAs
were
nonfunctional
only
few
could
act
chromatin
remodeling,
over
last
decades,
research
has
revealed
they
multifaceted,
versatile
regulators
most
processes.
Among
various
RNAs,
long
non-coding
(LncRNAs)
regulate
multiple
biological
processes
can
even
impact
fate.
In
this
sense,
subcellular
localization
lncRNAs
is
primary
determinant
their
functions.
It
affects
behavior
by
limiting
potential
partner
which
process
it
affect.
The
fine-tuned
activity
also
tissue-specific
modulated
Language: Английский