Effect of Liquid Marble 3D Culture System on In Vitro Maturation and Embryo Development of Prepubertal Goat Oocytes

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 188 - 188

Published: Jan. 12, 2025

Suboptimal culture conditions during in vitro maturation (IVM) affect oocyte developmental competence and the viability of resulting embryo. Three-dimensional (3D) systems provide a more biologically appropriate environment compared to traditional two-dimensional (2D) cultures. The aim this study was evaluate effect liquid marble (LM) microbioreactors as 3D system on IVM subsequent embryo development prepubertal goat oocytes. cumulus–oocyte complexes (COCs) recovered from ovaries underwent drops under oil (the 2D control group) LM group). After IVM, oocytes were parthenogenetically activated cultured until blastocyst stage. groups showed similar rates nuclear (52.17% 44.12%) formation (10.64% 10.10%). Reactive oxygen species glutathione levels density transzonal projections (TZPs) did not differ between groups. increased mitochondrial activity modified organization these organelles cytoplasm group. microbioreactor demonstrated ability improve status harmful for development. Therefore, could be used cost-effective

Language: Английский

Blastocyst exposure to plastic during mice in vitro fertilization impacts placental development

Reproductive Toxicology, Journal Year: 2025, Volume and Issue: 132, P. 108856 - 108856

Published: Feb. 12, 2025

Language: Английский

Assessment of bisphenol accumulation from disposable devices used sequentially in IVF routine procedures

Reproductive BioMedicine Online, Journal Year: 2024, Volume and Issue: 50(1), P. 104431 - 104431

Published: Aug. 29, 2024

Language: Английский