Molecular surveillance of resistance mutations in invasive populations of Spodoptera frugiperda in Europe, for evidence‐based pest control DOI Creative Commons
Konstantinos Mavridis, Vasiliki Evangelοu,

Alexandra M Grigoriadou

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract BACKGROUND The invasive fall armyworm ( Spodoptera frugiperda , FAW), a highly destructive pest affecting more than 350 plant species, has recently invaded Europe raising urgent management concerns. Insecticide resistance profiling is essential to support evidence‐based control strategies. In this study, we analyzed target‐site insecticide mutations in FAW populations from Greece inform addition, DNA barcoding through cytochrome oxidase subunit 1 COI ) gene sequencing was used trace the pest's geographic origin and potential invasion pathways. RESULTS All specimens were identified as rice strain, exhibiting two almost balanced haplotypes (Haplotype 1: 58.6%; Haplotype 2: 41.4%), suggesting likely single, genetically diverse source population. Resistance‐associated ABCC2 (A > G single‐nucleotide polymorphism (SNP); up 80.9%) Ace‐1 (F290V: 37.5%; A201S: 3.85%), conferring Bacillus thuringiensis (Bt) organophosphates/carbamates, respectively. By contrast, no resistance‐associated detected for other key insecticides (diamides, pyrethroids, oxadiazines, spinosyns, avermectins), their current efficacy Greece. CONCLUSION This study provides critical baseline monitoring Europe, supporting development of sustainable integrated strategies line with European Union Green Deal. Continuous molecular diagnostics, alongside complementary bioassays, recommended mitigate impact on agriculture. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Practical resistance of fall armyworm to Cry1A.105+Cry2Ab2+Cry3Bb1 Bt maize in Colombia DOI
Oscar F. Santos‐Amaya,

Juan E. Hernandez-Plata,

Daniel A. Baron-Ortiz

et al.

Crop Protection, Journal Year: 2025, Volume and Issue: unknown, P. 107229 - 107229

Published: April 1, 2025

Language: Английский

Citations

0
Joint toxicity and control efficacy of chemical pesticides and insecticidal bacteria against Spodoptera frugiperda (Lepidoptera: Noctuidae) DOI

Huizi Wu,

Xinyan Pan,

Jun Shen

et al.

Journal of Asia-Pacific Entomology, Journal Year: 2025, Volume and Issue: unknown, P. 102414 - 102414

Published: April 1, 2025

Language: Английский

Citations

0
Contact toxicity, Electrophysiology, Anti-mating and Repellent effects of Piper guineense against Spodoptera frugiperda DOI
Mobolade D. Akinbuluma, Jacques A. Deere, Peter Roessingh

et al.

Published: April 28, 2025

Abstract The fall armyworm Spodoptera frugiperda (J. E. Smith) is a long-distance migratory pest, which invaded the African continent in 2016, causing enormous losses to agricultural crops, especially maize. Synthetic insecticides are primarily used management of S. frugiperda, but they leave residues on human food and animal feed also cause environmental hazards. Assessing toxicity plant extracts may offer more effective control can reduce excessive use synthetic insecticides. We evaluated crude ethanolic extract Piper guineense fruits for contact larvae determined lethal concentration (LC50) extract. Additionally, we conducted electrophysiological (EAG) experiment determine responses male female adult P. whether influenced mating, oviposition repellence frugiperda. found that caused significantly higher mortality than an ethanol control. Electrophysiologically, observed control, with some variations response between sexes. When checking repellent effect females wind tunnel, moved towards Taken together, our results confirm as potent could be incorporated integrated Future research should explore field scale.

Language: Английский

Citations

0
Screening and functional validation of the core detoxification genes conferring broad‐spectrum response to insecticides in Spodoptera frugiperda DOI
Zhihui Lu, Kai Lǚ, Yahong Li

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(7), P. 3491 - 3503

Published: March 1, 2024

Abstract Background Fall armyworm, Spodoptera frugiperda , a formidable agricultural pest, has developed resistance to various synthetic insecticides. However, how S. utilizes its limited energy and resources deal with insecticides remains largely unexplored. Results We utilized transcriptome sequencing decipher the broad‐spectrum adaptation mechanism of eight distinct modes‐of‐action. Analysis Venn diagram revealed that 1014 upregulated genes 778 downregulated were present in treated at least five different insecticides, compared control group. Exposure led significant upregulation cytochrome P450 monooxygenases (P450s), four UDP glucosyltransferases (UGTs), two glutathione‐ S ‐transferases (GSTs) ATP‐binding cassette transporters (ABCs). Among them, sfCYP340AD3 sfCYP4G74 demonstrated respond stress from six as evidenced by RNA interference toxicity bioassays. Furthermore, homology modeling molecular docking analyses showed possess strong binding affinities variety Conclusion Collectively, these findings battery core detoxification cope exposure This study also sheds light on identification efficient insecticidal targets gene development management strategies thereby facilitating sustainable this serious pest. © 2024 Society Chemical Industry.

Language: Английский

Citations

3
Molecular surveillance of resistance mutations in invasive populations of Spodoptera frugiperda in Europe, for evidence‐based pest control DOI Creative Commons
Konstantinos Mavridis, Vasiliki Evangelοu,

Alexandra M Grigoriadou

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract BACKGROUND The invasive fall armyworm ( Spodoptera frugiperda , FAW), a highly destructive pest affecting more than 350 plant species, has recently invaded Europe raising urgent management concerns. Insecticide resistance profiling is essential to support evidence‐based control strategies. In this study, we analyzed target‐site insecticide mutations in FAW populations from Greece inform addition, DNA barcoding through cytochrome oxidase subunit 1 COI ) gene sequencing was used trace the pest's geographic origin and potential invasion pathways. RESULTS All specimens were identified as rice strain, exhibiting two almost balanced haplotypes (Haplotype 1: 58.6%; Haplotype 2: 41.4%), suggesting likely single, genetically diverse source population. Resistance‐associated ABCC2 (A > G single‐nucleotide polymorphism (SNP); up 80.9%) Ace‐1 (F290V: 37.5%; A201S: 3.85%), conferring Bacillus thuringiensis (Bt) organophosphates/carbamates, respectively. By contrast, no resistance‐associated detected for other key insecticides (diamides, pyrethroids, oxadiazines, spinosyns, avermectins), their current efficacy Greece. CONCLUSION This study provides critical baseline monitoring Europe, supporting development of sustainable integrated strategies line with European Union Green Deal. Continuous molecular diagnostics, alongside complementary bioassays, recommended mitigate impact on agriculture. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Citations

0