The nasal mycobiome of individuals with allergic rhinitis and asthma differs from that of healthy controls in composition, structure and function DOI Creative Commons
Marcos Pérez‐Losada, Eduardo Castro‐Nallar,

Jenaro García-Huidobro

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 17, 2024

Allergic rhinitis (AR) and asthma (AS) are two of the most common chronic respiratory diseases a major public health concern. Multiple studies have demonstrated role nasal bacteriome in AR AS, but little is known about airway mycobiome its potential association to inflammatory diseases. Here we used internal transcriber spacers (ITS) 1 2 high-throughput sequencing characterize 339 individuals with AR, (ARAS), AS healthy controls (CT). Seven ten 14 abundant fungal genera ( Malassezia, Alternaria, Cladosporium, Penicillium, Wallemia, Rhodotorula, Sporobolomyces, Naganishia, Vishniacozyma , nd Filobasidium ) cavity differed significantly p ≤ 0.049) between or ARAS, CT. However, none same varied three disease groups. The mycobiomes ARAS patients showed highest intra-group diversity, while CT lowest. Alpha-diversity indices microbial richness evenness only 0.024) CT, all groups significant differences 0.0004) structure (i.e., beta-diversity indices) when compared samples. Thirty metabolic pathways (PICRUSt2) were differentially (Wald’s test) patients, them associated 5-aminoimidazole ribonucleotide (AIR) biosynthesis over (log2 Fold Change >0.75) group. AIR has been pathogenesis plants. Spiec-Easi networks among groups, more similar interactions their members than those mycobiome; this suggests allergic may disrupt connectivity cavity. This study contributes valuable data results understand relationships allergy-related conditions. It demonstrates for first time that mycobiota varies during (with without comorbid asthma) reveals specific taxa, relate disease.

Language: Английский

GATA1-deficient human pluripotent stem cells generate neutrophils with improved antifungal immunity that is mediated by the integrin CD18 DOI Creative Commons
Andrew S. Wagner, Frances M. Smith, David A. Bennin

et al.

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(2), P. e1012654 - e1012654

Published: Feb. 3, 2025

Neutrophils are critical for host defense against fungi. However, the short life span and lack of genetic tractability primary human neutrophils has limited in vitro analysis neutrophil-fungal interactions. Human induced pluripotent stem cell (iPSC)-derived (iNeutrophils) provide a genetically tractable system to study responses neutrophils. Here, we show that deletion transcription factor GATA1 from iPSCs results iNeutrophils with improved antifungal activity Aspergillus fumigatus. GATA1-knockout (KO) have increased maturation, pattern recognition receptor expression neutrophil effector functions compared wild-type iNeutrophils. also shift their metabolism following stimulation fungal β-glucan pentose phosphate pathway (PPP), similar Furthermore, integrin CD18 attenuates ability GATA1-KO kill A. fumigatus but is not necessary metabolic shift. Collectively, these findings support as robust immunity identified specific roles response.

Language: Английский

Citations

1
Influence of cell bioenergetics on host-pathogen interaction in the lung DOI Creative Commons
Gaurav Kumar Lohia, Sebastián A. Riquelme

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 3, 2025

Pulmonary diseases, arising from infections caused by bacteria, fungi, and viruses, or stemming underlying genetic factors are one of the leading causes mortality in humans, accounting for millions deaths every year. At onset pulmonary crucial roles played phagocytic immune cells, particularly tissue-resident macrophages, regulating response at mucosal barrier. Recent strides have illuminated pivotal role host bioenergetics modulated metabolites derived both pathogens hosts influencing pathophysiology major organs. Their influence extends to processes such as infiltration activation polarization phenomenon. Furthermore, host-derived metabolites, itaconate, contribute promotion anti-inflammatory responses, thereby preventing immunopathology facilitating preservation niches thrive long-term. This review explores recent advancements field immunometabolism, with a particular emphasis on intricacies disease progression bacteria P. aeruginosa, M. tuberculosis S. aureus fungi like C. albicans.

Language: Английский

Citations

0
Metabolic homeostasis in fungal infections from the perspective of pathogens, immune cells, and whole-body systems DOI
Harshini Weerasinghe, Helen Stölting, Adam J. Rose

et al.

Microbiology and Molecular Biology Reviews, Journal Year: 2024, Volume and Issue: 88(3)

Published: Sept. 4, 2024

SUMMARY The ability to overcome metabolic stress is a major determinant of outcomes during infections. Pathogens face nutrient and oxygen deprivation in host niches their encounter with immune cells. Immune cells require adaptations for producing antimicrobial compounds mounting antifungal inflammation. Infection also triggers systemic changes organ metabolism energy expenditure that range from an enhanced produce robust response reduced as infection progresses, which coincides dysfunction. Competition nutrients between hosts pathogens means successful survival recovery balance elimination the pathogen by systems (resistance), doing so minimal damage tissues organs (tolerance). Here, we discuss our current knowledge pathogen, cell fungal infections, impact disorders, such obesity diabetes. We put forward idea that, while use regulation proliferation responses (i.e., resistance) has been growing over years, need study mechanisms control tolerance pathogens. A comprehensive understanding how resistance interventions may provide insights into therapeutic strategies could be used adjunctly drugs improve patient outcomes.

Language: Английский

Citations

2
GATA1-deficient human pluripotent stem cells generate neutrophils with improved antifungal immunity that is mediated by the integrin CD18 DOI Creative Commons
Andrew S. Wagner, Frances M. Smith, David A. Bennin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Abstract Neutrophils are critical for host defense against fungi. However, the short life span and lack of genetic tractability primary human neutrophils has limited in vitro analysis neutrophil-fungal interactions. Human induced pluripotent stem cell (iPSC)-derived (iNeutrophils) a genetically tractable alternative to neutrophils. Here, we show that deletion transcription factor GATA1 from iPSCs results iNeutrophils with improved antifungal activity Aspergillus fumigatus . knockout (KO) have increased maturation, pattern recognition receptor expression more readily execute neutrophil effector functions compared wild-type iNeutrophils. also shift their metabolism following stimulation fungal β-glucan, including an upregulation pentose phosphate pathway (PPP), similar Furthermore, integrin CD18 attenuates ability GATA1-KO kill A. but is not necessary PPP. Collectively, these findings support as robust system study immunity identified specific roles response. Author Summary important first responders infections, understanding essential better elucidating disease dynamics. Primary lived do permit manipulation, limiting use interactions analyses. In this report GATA1-deficient generate mature than display pathogen We receptors cells at levels comparable Deletion blocks control growth , demonstrating role iNeutrophil activity. model immunity.

Language: Английский

Citations

1
The nasal mycobiome of individuals with allergic rhinitis and asthma differs from that of healthy controls in composition, structure and function DOI Creative Commons
Marcos Pérez‐Losada, Eduardo Castro‐Nallar,

Jenaro García-Huidobro

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 17, 2024

Allergic rhinitis (AR) and asthma (AS) are two of the most common chronic respiratory diseases a major public health concern. Multiple studies have demonstrated role nasal bacteriome in AR AS, but little is known about airway mycobiome its potential association to inflammatory diseases. Here we used internal transcriber spacers (ITS) 1 2 high-throughput sequencing characterize 339 individuals with AR, (ARAS), AS healthy controls (CT). Seven ten 14 abundant fungal genera ( Malassezia, Alternaria, Cladosporium, Penicillium, Wallemia, Rhodotorula, Sporobolomyces, Naganishia, Vishniacozyma , nd Filobasidium ) cavity differed significantly p ≤ 0.049) between or ARAS, CT. However, none same varied three disease groups. The mycobiomes ARAS patients showed highest intra-group diversity, while CT lowest. Alpha-diversity indices microbial richness evenness only 0.024) CT, all groups significant differences 0.0004) structure (i.e., beta-diversity indices) when compared samples. Thirty metabolic pathways (PICRUSt2) were differentially (Wald’s test) patients, them associated 5-aminoimidazole ribonucleotide (AIR) biosynthesis over (log2 Fold Change >0.75) group. AIR has been pathogenesis plants. Spiec-Easi networks among groups, more similar interactions their members than those mycobiome; this suggests allergic may disrupt connectivity cavity. This study contributes valuable data results understand relationships allergy-related conditions. It demonstrates for first time that mycobiota varies during (with without comorbid asthma) reveals specific taxa, relate disease.

Language: Английский

Citations

0