Clinical & Translational Oncology, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 25, 2024
Language: Английский
Life Sciences, Journal Year: 2024, Volume and Issue: 348, P. 122691 - 122691
Published: May 5, 2024
Language: Английский
Citations
8
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 14
Published: Jan. 9, 2024
Introduction Abdominal obesity increases the risk of developing ovarian cancer but molecular mechanisms how supports development remain unknown. Here we investigated impact on immune cell and gene expression profiles distinct abdominal tissues, focusing peritoneal serous fluid (PSF) omental fat band (OFB) as critical determinants for dissemination metastases early metastatic events within cavity. Methods Female C57BL/6 mice were fed a low-fat (LFD) or high-fat diet (HFD) 12 weeks until body weights in HFD group significantly higher displayed an impaired glucose tolerance. Then injected with murine cells (MOSE-LTICv) while remaining their diets. After 21 days, sacrificed, tumor burden was evaluated tissues harvested. The composition changes PSF OFB by flow cytometry qPCR RT2-profiler PCR arrays confirmed qRT-PCR, respectively. Other adipose including parametrial retroperitoneal white well blood also investigated. Results While limited effects observed other feeding led to OFB: depletion B increase myeloid-derived suppressor (MDSC) mono/granulocytes, generating pro-inflammatory environments increased cyto- chemokines, genes supporting adhesion, survival, growth, suppression apoptosis. This associated compared LFD. Changes cellular genetic often exacerbated HFD. There large overlap that modulated both cells, suggesting this ‘genetic fingerprint’ is important OFB. Discussion In accordance ‘seed soil’ theory, our studies show contributes generation environment survival disseminating enhances adhesion through extracellular matrix proteins modulators such fibronectin 1 collagen I growth invasion Tenacin C. identified could potentially be used targets prevention strategies lower women obesity.
Language: Английский
Citations
4
Medicine, Journal Year: 2024, Volume and Issue: 103(11), P. e37460 - e37460
Published: March 15, 2024
Cholangiocarcinoma (CHOL) is a race malignant cancer arising from bile duct epithelial cells in clinical practice. C-X-C motif chemokine ligand 3 (CXCL3) member of chemokines family, which participates the pathogenesis various tumors. However, association between CXCL3 and CHOL unclear. This present study was to assess role expression progress CHOL. TIMER, GEPIA, UALCAN, GSCA, LinkedOmics, Metascape STRING databases were performed evaluate biological significances for with patients including expression, clinicopathological factors, immune cell infiltration, GO enrichment KEGG pathway analyses, as well PPI network analysis. The immunohistochemistry analysis tissue microarray conducted detect protein level, subcellular localization, factors prognosis mRNA levels markedly increased tissues. overexpression strongly associated maximum tumor diameter Additionally, there negative correlations monocyte Th17. Low infiltration neutrophil indicated significantly shorter cumulative survival patients. And arm-level deletion CD8+ T cell. Furthermore, functional suggested that its genes mainly enriched chemotaxis, secretory granule membrane, cytokine activity IL-17 signaling pathway. might potentially participate carcinogenesis CHOL, provided direction future research on mechanism
Language: Английский
Citations
4
iScience, Journal Year: 2024, Volume and Issue: 27(7), P. 110224 - 110224
Published: June 13, 2024
Cancer-associated fibroblasts (CAFs) play a significant role in tumor development and treatment failure, yet the precise mechanisms underlying their contribution to renal cell carcinoma (RCC) remains underexplored. This study explored interaction between CAFs cells, related mechanisms. isolated from tissues promoted progression drugs resistance both
Language: Английский
Citations
4
Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)
Published: April 9, 2025
Language: Английский
Citations
0
Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: unknown, P. 155978 - 155978
Published: April 1, 2025
Language: Английский
Citations
0
Heliyon, Journal Year: 2024, Volume and Issue: 10(15), P. e35200 - e35200
Published: July 26, 2024
Breast cancer has been reported to transcend lung as the most commonly diagnosed in women all over world. Adipocytes, serving energy storage and endocrine cells, are major stromal cells breast. Cancer-associated adipocytes (CAAs) adjacent dedifferentiated located at invasive front of human breast tumors. Adipocytes can transform into CAA phenotype with morphological biological changes under remodeling cells. CAAs play an essential role progression, including tumor microenvironment (TME), regulating immunity, interacting possess peculiar secretomes accordingly capable promote proliferation, invasiveness, angiogenesis, metastasis, immune escape, drug resistance There is a complex coordinated crosstalk among CAAs, release variety cytokines, IL-6, IL-8, IL-1β, CCL5, CCL2, VEGF, G-CSF, IGF-1, IGFBP, thereby promoting cell recruitment macrophage polarization, ultimately stimulating malignant behaviors Here, we aim provide comprehensive description CAA-derived their impact on behaviors, regulation, diagnosis, treatment. A deeper understanding performance interactions specific TME populations will better strategies for treatment reconstruction after mastectomy.
Language: Английский
Citations
3
Cancers, Journal Year: 2024, Volume and Issue: 16(16), P. 2910 - 2910
Published: Aug. 22, 2024
This study evaluated the paracrine signaling between breast carcinoma-associated fibroblasts (CAFs) and cancer (BCa) cells. Resolving cell–cell communication in BCa tumor microenvironment (TME) will aid development of new therapeutics. Here, we utilized our patented TAME (tissue architecture engineering) 3D culture microphysiological system, which is a suitable pathomimetic avatar for TME. We cultured cells CAFs either alone or together cocultures found that when cocultured, enhanced invasive characteristics cells, as shown by increased proliferation spread into surrounding matrix. Secretome analysis from cultures revealed relatively high secretion IL-6 CAFs. A marked increase granulocyte macrophage-colony stimulating factor (GM-CSF) carcinoma were coculture was also observed. theorized CAF-secreted functions manner to induce GM-CSF expression confirmed evaluating activation STAT3 gene exposed CAF-conditioned media (CAF-CM). In addition, treatment with cell-CM yielded brief upregulation followed decrease, indicating tightly regulated control Secretion drives turn GM-CSF. As result, cell-secreted upregulate inflammation-associated genes such IL-6, IL-6R IL-8, thereby forming positive feedback loop. propose tight regulation may be novel regulatory pathway target disrupting CAF:BCa cell symbiotic relationship. These data provide yet another piece network governing
Language: Английский
Citations
2
Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 170, P. 116016 - 116016
Published: Dec. 20, 2023
Ovarian cancer (OC) is the most lethal gynecological malignancy. Frequent peritoneal dissemination main cause of low survival rate. Guizhi-Fuling Wan (GZFL) a classical traditional Chinese herbal formula that has been clinically used for treating ovarian with good outcome. However, its therapeutic mechanism OC not clearly elucidated.
Language: Английский
Citations
4
Published: Jan. 1, 2024
Breast cancer has been reported to transcend lung as the most commonly diagnosed in women all over world. Adipocytes, serving energy storage and endocrine cells, are major stromal cells breast. Cancer-associated adipocytes (CAAs) adjacent dedifferentiated located at invasive front of human breast tumors. Adipocytes can transform into CAA phenotype with morphological biological changes under remodeling cells. CAAs play an essential role progression, including tumor microenvironment (TME), regulating immunity, interacting possess peculiar secretomes accordingly capable promote proliferation, invasiveness, angiogenesis, metastasis, immune escape, drug resistance There is a complex coordinated crosstalk among CAAs, release variety cytokines, IL-6, IL-8, IL-1β, CCL5, CCL2, VEGF, G-CSF, IGF-1, IGFBP, thereby promoting cell recruitment macrophage polarization, ultimately stimulating malignant behaviors Here, we aim provide comprehensive description CAA-derived their impact on behaviors, regulation, diagnosis, treatment. A deeper understanding performance interactions specific TME populations will better strategies for treatment reconstruction after mastectomy.
Language: Английский
Citations
0