All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants DOI Creative Commons
Aziz Ul Ikram, Shoaib Khan, Faisal Islam

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Unlike animals, plants are unable to move and lack specialized immune cells circulating antibodies. As a result, they always threatened by large number of microbial pathogens harmful pests that can significantly reduce crop yield worldwide. Therefore, the development new strategies control them is essential mitigate increasing risk crops lost plant diseases. Recent developments in genetic engineering, including efficient gene manipulation transformation methods, editing synthetic biology, coupled with understanding pathogenicity immunity, both at molecular genomic levels, have enhanced capabilities develop disease resistance plants. This review comprehensively explains fundamental mechanisms underlying tug-of-war between hosts, provides detailed overview different for developing Additionally, it summary potential genes be employed breeding key combat wide range pests, fungi, oomycetes, bacteria, viruses, nematodes, insects. Furthermore, this addresses limitations associated these their possible solutions. Finally, discusses future perspectives producing durable broad-spectrum resistance.

Language: Английский

Regulatory networks in plant responses to drought and cold stress DOI Creative Commons
June‐Sik Kim, Satoshi Kidokoro, Kazuko Yamaguchi‐Shinozaki

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(1), P. 170 - 189

Published: March 21, 2024

Abstract Drought and cold represent distinct types of abiotic stress, each initiating unique primary signaling pathways in response to dehydration temperature changes, respectively. However, a convergence at the gene regulatory level is observed where common set stress-responsive genes activated mitigate impacts both stresses. In this review, we explore these intricate networks, illustrating how plants coordinate stress signals into collective transcriptional strategy. We delve molecular mechanisms perception, signaling, activation pathways, with focus on insights gained from model species. By elucidating shared aspects plant responses drought cold, provide insight adaptive strategies plants, paving way for engineering stress-resilient crop varieties that can withstand changing climate.

Language: Английский

Citations

61
AP2/ERF transcription factors and their functions in Arabidopsis responses to abiotic stresses DOI
Kai Wang, Hongqing Guo, Yanhai Yin

et al.

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 222, P. 105763 - 105763

Published: March 31, 2024

Language: Английский

Citations

24
The elite haplotype OsGATA8-H coordinates nitrogen uptake and productive tiller formation in rice DOI Creative Commons
Wei Wu, Oliver Xiaoou Dong, Gaoming Chen

et al.

Nature Genetics, Journal Year: 2024, Volume and Issue: 56(7), P. 1516 - 1526

Published: June 13, 2024

Abstract Excessive nitrogen promotes the formation of nonproductive tillers in rice, which decreases use efficiency (NUE). Developing high-NUE rice cultivars through balancing uptake and productive remains a long-standing challenge, yet how these two processes are coordinated elusive. Here we identify transcription factor OsGATA8 as key coordinator tiller rice. negatively regulates by repressing ammonium transporter gene OsAMT3.2 . Meanwhile, it OsTCP19 , negative modulator tillering. We -H haplotype with enhanced higher proportion tillers. The geographical distribution OsGATA8- H its frequency change historical accessions suggest adaption to fertile soil. Overall, this study provides molecular evolutionary insights into regulation NUE facilitates breeding NUE.

Language: Английский

Citations

11
Prime editing: Mechanism insight and recent applications in plants DOI Creative Commons
Tien Van Vu,

Ngan Thi Nguyen,

Jihae Kim

et al.

Plant Biotechnology Journal, Journal Year: 2023, Volume and Issue: 22(1), P. 19 - 36

Published: Oct. 4, 2023

Summary Prime editing (PE) technology utilizes an extended prime guide RNA (pegRNA) to direct a fusion peptide consisting of nCas9 (H840) and reverse transcriptase (RT) specific location in the genome. This enables installation base changes at targeted site using portion pegRNA through RT activity. The resulting product reaction forms 3′ flap, which can be incorporated into genomic series biochemical steps involving DNA repair synthesis pathways. PE has demonstrated its effectiveness achieving almost all precise gene editing, such as conversions (all types), sequence insertions deletions, chromosomal translocation inversion long insertion safe harbour sites within In plant science, could serve groundbreaking tool for allowing creation desired alleles improve crop varieties. Nevertheless, application encountered limitations due efficiency constraints, particularly dicotyledonous plants. this review, we discuss step‐by‐step mechanism PE, shedding light on critical aspects each step while suggesting possible solutions enhance efficiency. Additionally, present overview recent advancements future perspectives research specifically focused plants, examining key technical considerations applications.

Language: Английский

Citations

21
CRISPR/Cas-mediated genome editing for efficient tomato breeding: past achievements and future directions DOI
Muhammad Naeem, Wajid Zaman, Saddam Saqib

et al.

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 172, P. 277 - 288

Published: July 26, 2024

Language: Английский

Citations

7
Developing a CRISPR/FrCas9 system for core promoter editing in rice DOI Creative Commons
Hui Wang,

Jian Ding,

Jingyan Zhu

et al.

aBIOTECH, Journal Year: 2024, Volume and Issue: 5(2), P. 189 - 195

Published: April 22, 2024

Small mutations in the core promoter region of a gene may result substantial changes expression strengths. However, targeting TA-rich sequences promoters pose challenge for Cas9 variants such as SpCas9 and other G-rich PAM-compatible Cas9s. In this study, we engineered unique FrCas9 system derived from

Language: Английский

Citations

4
Rice Promoter Editing: An Efficient Genetic Improvement Strategy DOI Creative Commons
Bowen Wu,

Hangfei Luo,

Zhongbo Chen

et al.

Rice, Journal Year: 2024, Volume and Issue: 17(1)

Published: Aug. 30, 2024

Gene expression levels in rice (Oryza sativa L.) and other plant species are determined by the promoters, which directly control phenotypic characteristics. As essential components of genes, promoters regulate intensity, location, timing gene expression. They contain numerous regulatory elements serve as binding sites for proteins that modulate transcription, including transcription factors RNA polymerases. Genome editing can alter promoter sequences, thereby precisely modifying patterns specific ultimately affecting morphology, quality, resistance rice. This paper summarizes research on conducted recent years, focusing improvements yield, heading date, disease resistance. It is expected to inform application encourage further development crop genetic improvement with promote.

Language: Английский

Citations

4
CRISPR/Cas in Grapevine Genome Editing: The Best Is Yet to Come DOI Creative Commons
Chong Ren,

Mohamed Salaheldin Mokhtar Mohamed,

Nuremanguli Aini

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(9), P. 965 - 965

Published: Sept. 11, 2024

The advent of Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR-associated (Cas) proteins as a revolutionary innovation in genome editing has greatly promoted targeted modification and trait improvement most plant species. For grapevine (Vitis vinifera L.), perennial woody species, CRISPR/Cas is an extremely promising technique for genetic short period. Advances have been achieved by using CRISPR technology recent years, which promises to accelerate grapevine. In this review, we describe the development advances CRISPR/Cas9 its orthologs variants. We summarize applications discuss challenges facing well possible strategies that could be used improve addition, outline future perspectives model system, precise editing, accelerated improvement, transgene-free editing. believe will play more important role exciting bright expected economically significant

Language: Английский

Citations

4
Genome Editing: A Novel Breeding Strategy for Enhanced Stress Tolerance and Quality in Oilseed Crops DOI
V. Mohan Murali Achary, Sarah Hearne,

Anindya Bandyopadhyay

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Harnessing promoter elements to enhance gene editing in plants: perspectives and advances DOI Creative Commons
Nikita Gondalia, Luis Felipe Quiroz, Linyi Lai

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Summary Genome‐edited plants, endowed with climate‐smart traits, have been promoted as tools for strengthening resilience against climate change. Successful plant gene editing (GE) requires precise regulation of the GE machinery, a process controlled by promoters, which drives its transcription through interactions factors (TFs) and RNA polymerase. While constitutive promoters are extensively used in constructs, their limitations highlight need alternative approaches. This review emphasizes promise tissue/organ specific well inducible enable targeted spatiotemporal manner no effects on other tissues. Advances synthetic biology paved way creation offering refined control over expression augmenting potential GE. The integration these novel systems presents significant opportunities conditional genome editing. Moreover, advent bioinformatic artificial intelligence is revolutionizing characterization regulatory elements, enhancing our understanding roles plants. Thus, this provides insights into strategic use promoter to enhance precision, efficiency specificity GE, setting stage innovative crop improvement strategies.

Language: Английский

Citations

0