Advances in mass spectrometry imaging for plant metabolomics—Expanding the analytical toolbox

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(5), P. 2168 - 2180

Published: July 11, 2024

SUMMARY Mass spectrometry imaging (MSI) has become increasingly popular in plant science due to its ability characterize complex chemical, spatial, and temporal aspects of metabolism. Over the past decade, as emerging unique features various MSI techniques have continued support new discoveries studies metabolism closely associated with function physiology, spatial metabolomics based on positioned it at forefront metabolic studies, providing opportunity for far higher resolution than was previously available. Despite these efforts, profound challenges levels resolution, sensitivity, quantitative ability, chemical confidence, isomer discrimination, multi‐omics integration, undoubtedly remain. In this Perspective, we provide a contemporary overview emergent widely used sciences, particular emphasis recent advances methodological breakthroughs. Having established detailed context MSI, outline both golden opportunities key currently facing metabolomics, presenting our vision how enormous potential technologies will contribute progress coming years.

Language: Английский

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Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 28, 2025

Abstract This study presents an investigation of the chemical composition Aloe vera leaf tissue with a focus on spatial distribution compounds. The was studied using two mass spectrometry imaging techniques: silver-109 nanoparticles assisted laser desorption/ionization ( 109 AgNPs-LDI-MSI) and ablation-remote atmospheric pressure photoionization/chemical ionization (LARAPPI/CI-MSI) identification aided by ultra-high-performance liquid chromatography ultra-high-resolution (UHPLC-UHRMS) analysis. results showed abundance phenolic compounds antioxidant, antimicrobial, anti-inflammatory properties, making it beneficial food additive packaging material. Analysis provided information about potential changes in metabolic pathway expression different regions leaf.

Language: Английский

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Multi‐omic analyses unveil contrasting composition and spatial distribution of specialized metabolites in seeds of Camelina sativa and other Brassicaceae

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(3)

Published: Feb. 1, 2025

SUMMARY Seeds of Brassicaceae produce a large diversity beneficial and antinutritional specialized metabolites (SMs) that influence their quality provide resistance to stresses. While SM distribution has been described in leaves root tissues, limited information is available about spatiotemporal accumulation seeds. Camelina sativa (camelina) an oilseed cultivated for human animal nutrition industrial uses. we previously explored plasticity, no expression related proteins genes camelina In this study, used multi‐omic approach, integrating untargeted metabolomics, proteomics, transcriptomics investigate the synthesis, modification, degradation SMs accumulated seed tissues (seed coat, endosperm, embryo) at six developmental two germination stages. Metabolomic results showed distinct patterns pathways, highlighting significant contrasts composition spatial defense‐related glucosinolate (GSL) compounds among camelina, Arabidopsis thaliana , Brassica napus three closely species. Notably, thanks metabolomic proteomic/transcriptomic techniques variation GSL distributions was primarily driven by differences structure (metabolomics data) transport (transcriptomic proteomic mechanisms. Long‐chain C8–C11 methylsulfinylalkyl GSLs were predominantly coat while mid‐ short‐chain C3–C7 embryo. Characterizing dynamics provides valuable insights can guide development crops with optimized toxic metabolites, improving nutritional profiles.

Language: Английский

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Metabolite-driven mechanisms reveal chemical ecology of Lehmann Lovegrass (Eragrostis lehmanniana) invasion in North American semi-arid ecosystems

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 4, 2025

Invasive plants threaten global ecosystems, yet traditional analyses of functional traits cannot fully explain their dominance over co-occurring natives. Metabolomics offers insights into plant invasions, but single-technique studies often miss critical biochemical mechanisms. We employ a multimodal metabolomics approach (¹H NMR, LC MS/MS, FT-ICR-MS, and MALDI-MSI) to investigate the basis Lehmann lovegrass (Eragrostis lehmanniana) invasion in semi-arid North America, comparing it with native grass, Arizona cottontop (Digitaria californica). Our analysis reveals three metabolomic compared cottontop: Enhanced nitrogen allocation shoots, reduced defensive metabolites root layers; increased exudate modulation under stress conditions. These suggest succeeds through adaptation increasing aridity rather than direct competition, demonstrating nutrient-poor environments high phenotypic plasticity response aridity. This integrated provides new mechanistic ecology environmental change.

Language: Английский

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Plant metabolomics

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(6), P. 1651 - 1653

Published: Feb. 22, 2024

Abstract Plants are a treasure trove of metabolic compounds. The chemical diversity plant cells has developed and been maintained through evolution regulation, plays crucial role in physiology, development, adaption to changing environmental situations. Metabolomics, when combined with genomics proteomics, opened up unprecedented opportunities address the biological importance diversity. It also provided an avenue for engineering produce particular compound interest meet societal economical demands, important effort achieve sustainable development. This Special Issue therefore focuses on current trends metabolomics research, providing examples development analytical technologies, functional study metabolism, applications synthetic biology.

Language: Английский

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Born of frustration: the emergence of Camelina sativa as a platform for lipid biotechnology.

PLANT PHYSIOLOGY, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract The emerging crop Camelina sativa (L.) Crantz (camelina) is a Brassicaceae oilseed with rapidly growing reputation for the deployment of advanced lipid biotechnology and metabolic engineering. recognised by agronomists its traits including yield, oil/protein content, drought tolerance, limited input requirements, plasticity resilience. Its utility as platform engineering was then quickly recognised, biotechnologists have benefited from short life cycle facile genetic transformation, producing numerous transgenic interventions to modify seed content generate novel products. desire work plant that both model has driven expansion research resources camelina, increased availability genome other “-omics” data sets. Collectively these established camelina an ideal study regulation metabolism improvement. Furthermore, unique characteristics enables design-build-test-learn be transitioned controlled environment field. Complex synthesize accumulate high levels fatty acids modified oils in seeds, can deployed, tested undergo rounds iteration agronomically relevant environments. Engineered are now increasingly being developed used sustainably supply, improved nutrition, feed, biofuels fossil fuel replacements high-value chemical In this review, we provide summary acid synthesis oil assembly highlighting how discovery supports advance towards predictive manipulation produce desirable bio-based Further examples innovation improvement provided, describing technologies (e.g., modification (GM), gene editing (GE), RNAi, alongside GM GE stacking) applied new products denude undesirable traits. Focusing on production long chain polyunsaturated omega-3 describe transition commercial prototype. prospects structured triacylglycerol specified stereospecific positions also discussed, future outlook agronomic uptake biotechnology.

Language: Английский

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Promising mass spectrometry imaging: exploring microscale insights in food

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 32

Published: Jan. 16, 2025

This review focused on mass spectrometry imaging (MSI), a powerful tool in food analysis, covering its ion source schemes and procedures their applications quality, safety, nutrition to provide detailed insights into these aspects. The presented introduction both commonly used emerging ionization sources, including nanoparticle laser desorption/ionization (NPs-LDI), air flow-assisted (AFAI), desorption with through-hole alumina membrane (DIUTHAME), plasma-assisted (PALDI), low-temperature plasma (LTP). In the MSI process, particular emphasis was placed quantitative (QMSI) super-resolution algorithms. These two aspects synergistically enhanced MSI's analytical capabilities: QMSI enabled accurate relative absolute quantification, providing reliable data for composition while algorithms improved molecular spatial resolution, facilitating biomarker trace substance detection. outperformed conventional methods comprehensively exploring functional factors, discovery, monitoring processing/storage effects by discerning species distributions. However, challenges such as immature techniques, complex processing, non-standardized instruments, high costs existed. Future trends instrument enhancement, multispectral integration, analysis improvement were expected deepen our understanding of chemistry highlighting revolutionary potential research.

Language: Английский

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Differentiation of genome‐wide DNA methylation between japonica and indica rice

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(2)

Published: Jan. 1, 2025

Rice (Oryza sativa L.) subspecies japonica and indica show distinct morphological genetic differentiation. However, the differences in genome-wide DNA methylation its effects on gene expression metabolic levels between rice remain unclear. In this study, we investigated methylation, transcriptomes metabolomes of 12 representative accessions, to reveal differentiation subspecies. We detected 83 327 differentially methylated regions (DMRs) 14 903 DMR-associated genes two Indica showed significantly lower CG, CHG, CHH compared with rice. Subsequently, identified 5596 expressed subspecies, predominantly enriched pathways related carbohydrate amino acid metabolism. By integrating transcriptomic data, a significant correlation was established patterns level key agronomic Furthermore, multi-omics analyses that metabolism pathways, especially tricarboxylic (TCA) cycle metabolites, are remarkable These results provide foundation for future studies domestication improvement.

Language: Английский

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Current insights into plant volatile organic compound biosynthesis

Current Opinion in Plant Biology, Journal Year: 2025, Volume and Issue: 85, P. 102708 - 102708

Published: March 28, 2025

Language: Английский

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OzMALDI: A Gas-Phase, In-Source Ozonolysis Reaction for Efficient Double-Bond Assignment in Mass Spectrometry Imaging with Matrix-Assisted Laser Desorption/Ionization

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Lipids make up an important class of biomolecules with diverse structures and varied chemical functions. This diversity is a major challenge in analysis limits our understanding biological functions regulation. A way lipid isomers differ by double-bond (db) position, analyzing db-isomers especially challenging for mass spectrometry imaging (MSI). Ozonolysis can be used to determine the db-position has been paired MSI before. However, previous techniques require increased time allow gas-phase reactions within ion trap or mobility cell additional sample preparation offline ozonation. Here, we introduce new ozonolysis method inside matrix-assisted laser desorption-ionization (MALDI) source, termed OzMALDI, that simultaneously produces ozonides from all unsaturated lipids. allows us db-positions without adding reaction while maintaining high resolution provided Orbitrap MS. technique effective at determining multiple lipids containing polyunsaturated fatty acids, which limitation many techniques. OzMALDI-MSI was applied rat brain genetically engineered Camelina soybean seed samples, demonstrating utility this uncovering novel information.

Language: Английский

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