Drug Resistance Updates, Journal Year: 2025, Volume and Issue: 79, P. 101204 - 101204
Published: Jan. 21, 2025
Language: Английский
Antibiotics, Journal Year: 2023, Volume and Issue: 12(2), P. 234 - 234
Published: Jan. 21, 2023
Klebsiella pneumoniae is a Gram-negative opportunistic pathogen responsible for variety of community and hospital infections. Infections caused by carbapenem-resistant K. (CRKP) constitute major threat public health are strongly associated with high rates mortality, especially in immunocompromised critically ill patients. Adhesive fimbriae, capsule, lipopolysaccharide (LPS), siderophores or iron carriers the main virulence factors which contribute to pathogenicity pneumoniae. Colistin tigecycline some last resorts treatment CRKP Carbapenemase production, carbapenemase (KPC) metallo-β-lactamase (MBL), constitutes basic molecular mechanism emergence. Knowledge appearance crucial, as it can determine selection most suitable antimicrobial agent among those recently launched. Plazomicin, eravacycline, cefiderocol, temocillin, ceftolozane–tazobactam, imipenem–cilastatin/relebactam, meropenem–vaborbactam, ceftazidime–avibactam aztreonam–avibactam potent alternatives treating The aim current review highlight pathogenesis provide recent updates on epidemiology options.
Language: Английский
Citations
152
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: May 31, 2022
Abstract Klebsiella pneumoniae is a major cause of opportunistic healthcare-associated infections, which are increasingly complicated by the presence extended-spectrum beta-lactamases (ESBLs) and carbapenem resistance. We conducted year-long prospective surveillance study K. clinical isolates in hospital patients. Whole-genome sequence (WGS) data reveals diverse pathogen population, including other species within complex (18%). Several infections were caused variicola/K. hybrids, one shows evidence nosocomial transmission. A wide range antimicrobial resistance (AMR) phenotypes observed, genetic mechanisms identified (mainly plasmid-borne genes). ESBLs correlated with acquired AMR genes (median n = 10). Bacterial genomic features associated onset (OR 2.34, p 0.015) rhamnose-positive capsules 3.12, < 0.001). Virulence plasmid-encoded (aerobactin, hypermucoidy) observed at low-prevalence (<3%), mostly community-onset cases. WGS-confirmed transmission implicated just 10% cases, but strongly 21, 1 × 10 −11 ). estimate 28% risk onward for ESBL-positive strains vs 1.7% ESBL-negative strains. These indicate that hospitalised patients due largely to strains, an additional burden from nosocomially-transmitted community-acquired hypervirulent
Language: Английский
Citations
117
Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(3), P. 814 - 829
Published: Feb. 29, 2024
Abstract Epidemiological knowledge of circulating carbapenem-resistant Klebsiella pneumoniae (CRKP) is needed to develop effective strategies against this public health threat. Here we present a longitudinal analysis 1,017 CRKP isolates recovered from patients 40 hospitals across China between 2016 and 2020. Virulence gene capsule typing revealed expansion type KL64 (59.5%) alongside decreases in KL47 prevalence. Hypervirulent increased prevalence 28.2% 45.7% Phylogenetic spatiotemporal Beijing Shanghai as transmission hubs accounting for differential geographical strains China. Moderate frequency or O-antigen loss was also detected among isolates. Non-capsular were more susceptible phagocytosis, attenuated during mouse infections, but showed serum resistance biofilm formation. These findings give insight into serotype dynamics, revealing the importance monitoring shifts future development immunological infections.
Language: Английский
Citations
54
Vaccine, Journal Year: 2024, Volume and Issue: 42(19), P. S125 - S141
Published: March 19, 2024
Klebsiella pneumoniae causes community- and healthcare-associated infections in children adults. Globally 2019, an estimated 1.27 million (95% Uncertainty Interval [UI]: 0.91-1.71) 4.95 UI: 3.62-6.57) deaths were attributed to associated with bacterial antimicrobial resistance (AMR), respectively. K. was the second leading pathogen AMR resistant bacteria. Furthermore, rise of both hospital-acquired is a concern for neonates infants who are at high risk invasive disease. There limited antibiotic pipeline new antibiotics treat multidrug infections, vaccines targeted against considered be priority by World Health Organization. Vaccination pregnant women could reduce K.pneumoniae disease their young offspring. In addition, vulnerable children, adolescents adult populations underlying diseases such as immunosuppression from hematologic malignancy, chemotherapy, patients undergoing abdominal and/or urinary surgical procedures, or prolonged intensive care management also potential target groups vaccine. A 'Vaccine Value Profile' (VVP) K.pneumoniae, which contemplates vaccination protect babies birth through least three months age other high-risk populations, provides high-level, holistic assessment available information inform public health, economic societal value preventatives therapeutics. This VVP developed working group subject matter experts academia, non-profit organizations, public-private partnerships, multi-lateral collaboration stakeholders WHO. All contributors have extensive expertise on various elements collectively aimed identify current research knowledge gaps. The using only existing publicly information.
Language: Английский
Citations
21
Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(1)
Published: Feb. 23, 2022
The Klebsiella pneumoniae species complex (KpSC) is a leading cause of multidrug-resistant human infections. To better understand the potential contribution food as vehicle KpSC, we conducted multicentric study to define an optimal culture method for its recovery from matrices and characterize isolates phenotypically genotypically. Chicken meat (n = 160) salad 145) samples were collected in five European countries screened presence KpSC using culture-based zur-khe intergenic region (ZKIR) quantitative PCR (qPCR) methods. Enrichment buffered peptone water followed by streaking on Simmons citrate agar with inositol (44°C 48 h) was defined most suitable selective recovery. A high prevalence found chicken (60% 52% ZKIR qPCR approach, respectively) (30% 21%, samples. Genomic analyses revealed genetic diversity dominance phylogroups Kp1 (91%) Kp3 (6%). total 82% presented natural antimicrobial susceptibility phenotype genotype, only four CTX-M-15-producing detected. Notably, identical genotypes across samples-same type same country (15 cases), different types (1), two (1)-suggesting rates transmission within sector. Our provides novel isolation strategy reinforces view source colonization humans. IMPORTANCE Bacteria are ubiquitous, K. antibiotic-resistant infections Despite urgent public health threat represented pneumoniae, there lack knowledge sources subsequent infection This partly due absence standardized methods characterizing matrices. implements shows that members highly prevalent salads meat, reinforcing large low levels resistance detected, occurrence suggests sector, which need be further explored possible control strategies.
Language: Английский
Citations
44
Biological Research, Journal Year: 2024, Volume and Issue: 57(1)
Published: March 12, 2024
Abstract Background The convergence of hypervirulence and carbapenem resistance in the bacterial pathogen Klebsiella pneumoniae represents a critical global health concern. Hypervirulent K. (hvKp) strains, frequently from sequence type 23 (ST23) having K1 capsule, have been associated with severe community-acquired invasive infections. Although hvKp were initially restricted to Southeast Asia primarily antibiotic-sensitive, carbapenem-resistant infections are reported worldwide. Here, within carbapenemase production Enterobacterales surveillance system headed by Chilean Public Health Institute, we describe isolation Chile high-risk ST23 dual-carbapenemase-producing strain, which genes encoded single conjugative plasmid. Results Phenotypic molecular tests this strain revealed an extensive at least 15 antibiotic classes KPC-2 VIM-1 carbapenemases. Unexpectedly, isolate lacked hypermucoviscosity, challenging commonly used identification criteria. Complete genome sequencing analysis confirmed capsular type, KpVP-1 virulence plasmid, GIE492 ICEKp10 genomic islands carrying factors strongly hvKp. belonged globally disseminated clonal group CG23-I, it is unique, as formed clade apart previously acquired IncN plasmid highly South America (absent other genomes), but now including class-I integron bla VIM−1 genes. Notably, was able conjugate double E. coli recipient, conferring 1st -5th generation cephalosporins (including combinations beta-lactamase inhibitors), penicillins, monobactams, carbapenems. Conclusions We transmissible encoding carbapenemases, most beta-lactams. Furthermore, lack hypermucoviscosity argues against trait reliable marker. These findings highlight rapid evolution towards multi-drug globally, well importance plasmids mobile genetic elements convergence. In regard, approaches provide valuable support monitor obtain essential information on these priority pathogens elements.
Language: Английский
Citations
9
Microbial Genomics, Journal Year: 2025, Volume and Issue: 11(1)
Published: Jan. 15, 2025
Phylogenetic analyses are crucial for understanding microbial evolution and infectious disease transmission. Bacterial phylogenies often inferred from SNP alignments, with SNPs as the fundamental signal within these data. alignments can be reduced to a ‘strict core’ by removing those sites that do not have data present in every sample. However, sample size genome diversity increase, strict core shrink markedly, discarding potentially informative Here, we propose provide evidence support use of ‘soft tolerates some missing data, preserving more information phylogenetic analysis. Using large datasets Neisseria gonorrhoeae Salmonella enterica serovar Typhi, assess different thresholds. Our results show cores drastically reduce compared soft cores. In 10 000-genome alignment 95% yielded ten times than 100% core. Similar patterns were observed N. . We further evaluated accuracy built strict- soft-core using strong temporal signals. Soft-core generally outperformed producing trees displaying clock-like behaviour; instance, phylogeny had root-to-tip regression R 2 0.50 0.21 strict-core phylogeny. This study suggests strategies preferable large, diverse datasets. To facilitate this, developed Core-SNP-filter ( https://github.com/rrwick/Core-SNP-filter ), an open-source software tool generating whole-genome based on user-defined
Language: Английский
Citations
1
EBioMedicine, Journal Year: 2025, Volume and Issue: 115, P. 105683 - 105683
Published: April 6, 2025
Language: Английский
Citations
1
Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(4)
Published: June 20, 2023
Klebsiella pneumoniae (Kp) is an ecologically generalist bacterium as well opportunistic pathogen that responsible for hospital-acquired infections and a major contributor to the global burden of antimicrobial resistance. In last decades, limited advances have been made in use virulent phages alternatives or complements antibiotics are used treat Kp infections.
Language: Английский
Citations
19
Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(3)
Published: April 26, 2023
Klebsiella pneumoniae sequence type 14 (ST14) and ST15 caused outbreaks of CTX-M-15 and/or carbapenemase producers worldwide, but their phylogeny global dynamics remain unclear. We clarified the evolution K. clonal group (CG14) CG15 by analyzing capsular locus (KL), resistome, virulome, plasmidome public genomes (n = 481) de novo sequences 9) representing main sublineages circulating in Portugal. CG14 evolved independently within 6 subclades defined according to KL accessory genome. The 65) clade was structured two large monophyletic subclades, CG14-I (KL2, 86%) CG14-II (KL16, 14%), whose emergences were dated 1932 1911, respectively. Genes encoding extended-spectrum β-lactamase (ESBL), AmpC, carbapenemases mostly observed (71% versus 22%). 170) segregated into CG15-IA (KL19/KL106, 9%), CG15-IB (variable types, 6%), CG15-IIA (KL24, 43%) CG15-IIB (KL112, 37%). Most carried specific GyrA ParC mutations emerged from a common ancestor 1989. especially prevalent (68% 38% CG14) (92%). Plasmidome analysis revealed 27 predominant plasmid groups (PG), including particularly pervasive recombinant F-type 10), Col new types. While blaCTX-M-15 acquired multiple times high diversity mosaic plasmids, other antibiotic resistance genes (ARGs) dispersed IncL (blaOXA-48) or IncC (blaCMY/TEM-24) plasmids. first demonstrate an independent evolutionary trajectory for how acquisition KL, quinolone-resistance determining region (QRDR) (CG15), ARGs highly plasmids could have shaped expansion diversification particular (CG14-I CG15-IIA/IIB). IMPORTANCE represents major threat burden (ABR). Available studies explain origin, diversity, certain ABR populations mainly been focused on few (CGs) using phylogenetic core genome, genome being overlooked. Here, we provide unique insights CG15, poorly characterized CGs which contributed dissemination responsible first-line antibiotics such as β-lactams. Our results point out these highlight existence different Moreover, contribution turbulent flux (especially multireplicon F Col) adaptive traits (antibiotic metal tolerance genes) pangenome reflect exposure adaptation under selective pressures.
Language: Английский
Citations
15