Reconstructing NOD-like receptor alleles with high internal conservation in Podospora anserina using long-read sequencing DOI Creative Commons
S. Lorena Ament‐Velásquez, Brendan Furneaux,

Sonia Dheur

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

NOD-like receptors (NLRs) are intracellular immune that detect pathogen-associated cues and trigger defense mechanisms, including regulated cell death. In filamentous fungi, some NLRs mediate heterokaryon incompatibility, a self/non-self recognition process prevents the vegetative fusion of genetically distinct individuals, reducing risk parasitism. The het-d het-e in Podospora anserina highly polymorphic incompatibility genes ( het genes) whose products recognize different alleles het-c gene via sensor domain composed WD40 repeats. These repeats display unusually high sequence identity maintained by concerted evolution. However, sites within individual hypervariable under diversifying selection. Despite extensive genetic studies, inconsistencies reported have hindered functional evolutionary analyses. Here we demonstrate can be accurately reconstructed from long-read sequencing (Oxford Nanopore PacBio) data, but not Illumina-based assemblies. Functional usually formed 11 conserved repeats, with repeat combinations underlying same phenotypic reactions. Protein structure models suggest their folds into two 7-blade β-propellers as well three cryptic divergent at C-terminus. We additionally show one particular allele does an reaction common alleles, despite being 11-repeats long. Our findings provide robust foundation for future research molecular mechanisms dynamics NLRs, while also highlighting both fragility flexibility β -propellers domains.

Language: Английский

Emergence of the fungal immune system DOI Creative Commons
Asen Daskalov

iScience, Journal Year: 2023, Volume and Issue: 26(6), P. 106793 - 106793

Published: May 3, 2023

Investigation of fungal biology has been frequently motivated by the fact that many species are important plant and animal pathogens. Such efforts have contributed significantly toward our understanding pathogenic lifestyles (virulence factors strategies) interplay with host immune systems. In parallel, work on allorecognition systems leading to characterization regulated cell death determinants pathways, instrumental for emergent concept immunity. The uncovered evolutionary

Language: Английский

Citations

23
The human fungal pathogen Aspergillus fumigatus can produce the highest known number of meiotic crossovers DOI Creative Commons
Ben Auxier, Alfons J. M. Debets, Felicia Adelina Stanford

et al.

PLoS Biology, Journal Year: 2023, Volume and Issue: 21(9), P. e3002278 - e3002278

Published: Sept. 14, 2023

Sexual reproduction involving meiosis is essential in most eukaryotes. This produces offspring with novel genotypes, both by segregation of parental chromosomes as well crossovers between homologous chromosomes. A sexual cycle for the opportunistic human pathogenic fungus Aspergillus fumigatus known, but genetic consequences have remained unknown. Among other Aspergilli, it known that A. flavus has a moderately high recombination rate an average 4.2 per chromosome pair, whereas nidulans contrast higher 9.3 pair. Here, we show cross strains they produce 29.9 pair and large variation total map length across additional strain crosses. more than twice seen any organism, which discuss relation to model systems. We validate this through mapping resistance laboratory antifungal acriflavine using standing undescribed ABC efflux transporter. then demonstrate sufficient one common multidrug resistant haplotypes found cyp51A gene (TR34/L98H) crosses among parents harboring either 2 nearby variants, possibly explaining early spread such haplotypes. Our results suggest genomic studies species should reassess assumptions about linkage regions. The finding unparalleled crossover provides opportunities understand why these rates are not generally

Language: Английский

Citations

14
Giant transposons promote strain heterogeneity in a major fungal pathogen DOI Creative Commons
Emile Gluck‐Thaler, Adrian Forsythe, Charles Puerner

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 28, 2024

Fungal infections are difficult to prevent and treat in large part due strain heterogeneity. However, the genetic mechanisms driving pathogen variation remain poorly understood. Here, we determined extent which

Language: Английский

Citations

5
Reconstructing NOD-like receptor alleles with high internal conservation in Podospora anserina using long-read sequencing DOI Creative Commons
S. Lorena Ament‐Velásquez, Brendan Furneaux,

Sonia Dheur

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

NOD-like receptors (NLRs) are intracellular immune that detect pathogen-associated cues and trigger defense mechanisms, including regulated cell death. In filamentous fungi, some NLRs mediate heterokaryon incompatibility, a self/non-self recognition process prevents the vegetative fusion of genetically distinct individuals, reducing risk parasitism. The het-d het-e in Podospora anserina highly polymorphic incompatibility genes ( het genes) whose products recognize different alleles het-c gene via sensor domain composed WD40 repeats. These repeats display unusually high sequence identity maintained by concerted evolution. However, sites within individual hypervariable under diversifying selection. Despite extensive genetic studies, inconsistencies reported have hindered functional evolutionary analyses. Here we demonstrate can be accurately reconstructed from long-read sequencing (Oxford Nanopore PacBio) data, but not Illumina-based assemblies. Functional usually formed 11 conserved repeats, with repeat combinations underlying same phenotypic reactions. Protein structure models suggest their folds into two 7-blade β-propellers as well three cryptic divergent at C-terminus. We additionally show one particular allele does an reaction common alleles, despite being 11-repeats long. Our findings provide robust foundation for future research molecular mechanisms dynamics NLRs, while also highlighting both fragility flexibility β -propellers domains.

Language: Английский

Citations

0