het-Ballorecognition inPodospora anserinais determined by pseudo-allelic interaction of genes encoding a HET and lectin fold domain protein and a PII-like protein DOI Creative Commons
Corinne Clavé,

Sonia Dheur,

S. Lorena Ament‐Velásquez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 19, 2023

Abstract Filamentous fungi display allorecognition genes that trigger regulated cell death (RCD) when strains of unlike genotype fuse. Podospora anserina is one several model species for the study this process termed heterokaryon or vegetative incompatibility. Incompatibility restricts transmission mycoviruses between isolates. In P. , genetic analyses have identified nine incompatibility loci, het loci. Here we set out to clone controlling het-B displays two incompatible alleles, het-B1 and het-B2 . We find locus encompasses adjacent genes, Bh Bp exist as highly divergent allelic variants ( Bh1/Bh2 Bp1/Bp2 ) in haplotypes. encodes a protein with an N-terminal HET domain, inducing domain bearing homology Toll/interleukin-1 receptor (TIR) domains C-terminal predicted lectin fold. The product homologous PII-like proteins, family small trimeric proteins acting sensors adenine nucleotides bacteria. show although system appears genetically allelic, fact determined by non-allelic Bh1/Bp2 interaction while reciprocal Bh2/Bp1 plays no role fold BH determines recognition specificity. Population studies genome indicate under balancing selection trans-species polymorphism, highlighting evolutionary significance addition emphasizing anew central TIR-like fungal RCD, identifies novel players completes characterization entire gene species. Author summary Many cellular life forms systems protect individuality discriminate conspecific self from non-self. filamentous fungi, fusion events are check specific upon detection restrict mycovirus parasitism. good form allorecognition, harbors Previous revealed these can be immune functions other phyla including bacteria, plants animals. cloned het-B, last remained found it complex comprising (related TIR domains) BP known bacterial metabolite sensors. Intriguingly, despite apparent allelism, dictated Bh/Bp interaction. This stresses reoccurring involvement RCD signs completion loci species, enabling comparative analysis different architectures underlying allorecognition.

Language: Английский

The conceptual foundations of innate immunity: Taking stock 30 years later DOI Creative Commons
Thomas Pradeu, Bart P. H. J. Thomma, Stephen E. Girardin

et al.

Immunity, Journal Year: 2024, Volume and Issue: 57(4), P. 613 - 631

Published: April 1, 2024

SummaryWhile largely neglected over decades during which adaptive immunity captured most of the attention, innate immune mechanisms have now become central to our understanding immunology. Innate provides first barrier infection in vertebrates, and it is sole mechanism host defense invertebrates plants. also plays a critical role maintaining homeostasis, shaping microbiota, disease contexts such as cancer, neurodegeneration, metabolic syndromes, aging. The emergence field has led an expanded view system, no longer restricted vertebrates instead concerns all metazoans, plants, even prokaryotes. study given rise new concepts language. Here, we review history definition core immunity, discussing their value fruitfulness long run.

Language: Английский

Citations

35
Conservation and similarity of bacterial and eukaryotic innate immunity DOI
Hannah E. Ledvina, Aaron T. Whiteley

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(7), P. 420 - 434

Published: Feb. 28, 2024

Language: Английский

Citations

24
What are the 100 most cited fungal genera? DOI

C.S. Bhunjun,

Yijun Chen, Chayanard Phukhamsakda

et al.

Studies in Mycology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

23
TIR signaling activates caspase-like immunity in bacteria DOI
François Rousset, Ilya А. Osterman, Tali Scherf

et al.

Science, Journal Year: 2025, Volume and Issue: 387(6733), P. 510 - 516

Published: Jan. 30, 2025

Caspase family proteases and Toll/interleukin-1 receptor (TIR)-domain proteins have central roles in innate immunity regulated cell death humans. We describe a bacterial immune system comprising both caspase-like protease TIR-domain protein. found that the TIR protein, once it recognizes phage invasion, produces previously unknown signaling molecule adenosine 5′-diphosphate-cyclo[N7:1′′]-ribose (N7-cADPR). This specifically activates protease, which then indiscriminately degrades cellular to halt replication. The TIR-caspase defense system, we denote as type IV Thoeris, is abundant bacteria efficiently protects against propagation. Our study highlights diversity of TIR-produced molecules demonstrates by caspase an ancient mechanism immunity.

Language: Английский

Citations

5
Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon DOI Creative Commons
Ying Zheng, Benjamin Moorlach, Désirée Jakobs‐Schönwandt

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 25, 2025

In vertebrates and plants, dsRNA plays crucial roles as PAMP a mediator of RNAi. How higher fungi respond to is not known. We demonstrate that Magnaporthe oryzae (Mo), globally significant crop pathogen, internalizes across broad size range 21 about 3000 bp. Incubation fungal conidia with 10 ng/µL dsRNA, regardless or sequence, induced aberrant germ tube elongation, revealing strong sequence-unspecific effect in this fungus. Accordingly, the synthetic analogue poly(I:C) various sizes sequences elicited canonical stress pathways, including nuclear accumulation marker mitogen-activated protein kinase Hog1p production ROS. Leaf application cereal model species Brachypodium distachyon suppressed progression leaf blast disease. Notably, depends on doses, while pure sequence-specific effects were observed at low concentrations ( < 0.03 ng/µL). The protective further enhanced by maintaining gap least seven days between inoculation, stabilising alginate-chitosan nanoparticles. Overall, our study opens up additional possibilities for development use pesticides agriculture.

Language: Английский

Citations

2
Gasdermins as evolutionarily conserved executors of inflammation and cell death DOI
Kaiwen W. Chen, Petr Brož

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(9), P. 1394 - 1406

Published: Aug. 26, 2024

Language: Английский

Citations

15
Cytoplasmic calcium influx mediated by plant MLKLs confers TNL-triggered immunity DOI Creative Commons

Qiaochu Shen,

Keiichi Hasegawa,

Nicole Oelerich

et al.

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(4), P. 453 - 465.e6

Published: March 20, 2024

The plant homolog of vertebrate necroptosis inducer mixed-lineage kinase domain-like (MLKL) contributes to downstream steps in Toll-interleukin-1 receptor domain NLR (TNL)-receptor-triggered immunity. Here, we show that Arabidopsis MLKL1 (AtMLKL1) clusters into puncta at the plasma membrane upon TNL activation and this sub-cellular reorganization is dependent on signal transducer, EDS1. We find AtMLKLs confer TNL-triggered immunity parallel with RPW8-type HeLo-domain-containing NLRs (RNLs) AtMLKL N-terminal HeLo indispensable for both clustering. mediates cytoplasmic Ca

Language: Английский

Citations

12
Symbioses between fungi and bacteria: from mechanisms to impacts on biodiversity DOI Creative Commons
Teresa E. Pawlowska

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 80, P. 102496 - 102496

Published: June 13, 2024

Symbiotic interactions between fungi and bacteria range from positive to negative. They are ubiquitous in free-living as well host-associated microbial communities worldwide. Yet, the impact of fungal-bacterial symbioses on organization dynamics is uncertain. There two reasons for this uncertainty: (1) knowledge gaps understanding genetic mechanisms underpinning (2) prevailing interpretations ecological theory that favor antagonistic drivers stabilizing biological despite existence models emphasizing contributions interactions. This review synthesizes information common soil polymicrobial biofilms. The interdomain partnerships considered context relevant community ecology models, which discussed critically.

Language: Английский

Citations

10
Unveiling the intratumoral microbiota within cancer landscapes DOI Creative Commons
Shusheng Che, Zhiyong Yan, Yugong Feng

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 109893 - 109893

Published: May 3, 2024

Recent advances in cancer research have unveiled a significant yet previously underappreciated aspect of oncology: the presence and role intratumoral microbiota. These microbial residents, encompassing bacteria, fungi, viruses within tumor tissues, been found to exert considerable influence on development, progression, efficacy therapeutic interventions. This review aims synthesize these groundbreaking discoveries, providing an integrated overview identification, characterization, functional roles microbiota biology. We focus elucidating complex interactions between microorganisms microenvironment, highlighting their potential as novel biomarkers targets. The purpose this is offer comprehensive understanding dimension cancer, paving way for innovative approaches diagnosis treatment.

Language: Английский

Citations

9
The immune modules conserved across the tree of life: Towards a definition of ancestral immunity DOI Creative Commons
Aude Bernheim, Jean Cury, Enzo Z. Poirier

et al.

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(7), P. e3002717 - e3002717

Published: July 15, 2024

Immune defence mechanisms exist across the tree of life in such diversity that prokaryotic antiviral responses have historically been considered unrelated to eukaryotic immunity. Mechanisms divergent eukaryotes were similarly believed be largely clade specific. However, recent data indicate a subset modules (domains and proteins) from prokaryote systems are conserved populate many stages innate immune pathways. In this Essay, we propose notion ancestral immunity, which corresponds set between prokaryotes eukaryotes. After offering typology speculate on selective pressures could led differential conservation specific domains life. The exploration immunity is its infancy appears full promises illuminate evolution, also identify decipher economic, ecological, therapeutic importance.

Language: Английский

Citations

9