Reviewer #1 (Public Review): SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus DOI Open Access

Published: July 5, 2024

The epigenetic reader SntB was identified as an important transcriptional regulator of growth, development, and secondary metabolite synthesis in Aspergillus flavus. However, the underlying molecular mechanism is still unclear. In this study, sntB gene deletion (ΔsntB), complementary (Com-sntB), HA tag fused to (sntB-HA) strains were constructed by using homologous recombination method, respectively. Our results revealed that inhibited processes mycelia conidial production, sclerotia formation, aflatoxin synthesis, ability colonize host compared wild type (WT), defective phenotype knockout strain ΔsntB could be restored its Com-sntB. Chromatin immunoprecipitation sequencing (ChIP-seq) sntB-HA WT RNA (RNA-seq) played key roles oxidative stress response A. function catC (encode a catalase) further analyzed based on integration ChIP-seq RNA-seq. strain, relative expression level significantly higher than while secretory lipase encoding (G4B84_008359) down-regulated. Under oxidant menadione sodium bisulfite (MSB), obvious down-regulated catC. After gene, formation inhibited, ROS production increased strain. Results also showed inhibition rate MSB ΔcatC lower group AFB1 yield decreased under MSB. study potential machinery regulated fungal morphogenesis, mycotoxin anabolism, virulence through axle from bio-synthesis, i.e. H3K36me3 modification-SntB-Peroxisomes-Lipid hydrolysis-fungal bio-synthesis. shed light into mediated transcript regulation pathway anabolism virulence, which provided strategy for control contamination flavus aflatoxins.

Language: Английский

Molecular regulation of fungal secondary metabolism DOI
Wenbin Yu,

Rongqiang Pei,

Jingyi Zhou

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 39(8)

Published: May 20, 2023

Language: Английский

Citations

28
Aspergillus fumigatus mitogen-activated protein kinase MpkA is involved in gliotoxin production and self-protection DOI Creative Commons
Patrícia Alves de Castro, Camila Figueiredo Pinzan, Thaila Fernanda dos Reis

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 2, 2024

Abstract Aspergillus fumigatus is a saprophytic fungus that can cause variety of human diseases known as aspergillosis. Mycotoxin gliotoxin (GT) production important for its virulence and must be tightly regulated to avoid excess toxicity the fungus. GT self-protection by GliT oxidoreductase GtmA methyltransferase activities related subcellular localization these enzymes how sequestered from cytoplasm increased cell damage. Here, we show GliT:GFP GtmA:GFP are localized in vacuoles during production. The Mitogen-Activated Protein kinase MpkA essential self-protection, interacts physically with it necessary their regulation subsequent presence vacuoles. sensor histidine SlnA Sln1 modulation phosphorylation. Our work emphasizes importance compartmentalization cellular events self-defense.

Language: Английский

Citations

10
An Anatomy of Fungal Eye: Fungal Photoreceptors and Signalling Mechanisms DOI Creative Commons
Özlem Sarikaya Bayram, Özgür Bayram

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(5), P. 591 - 591

Published: May 19, 2023

Organisms have developed different features to capture or sense sunlight. Vertebrates evolved specialized organs (eyes) which contain a variety of photosensor cells that help them see the light aid orientation. Opsins are major photoreceptors found in vertebrate eye. Fungi, with more than five million estimated members, represent an important clade living organisms functions for sustainability life on our planet. Light signalling regulates range developmental and metabolic processes including asexual sporulation, sexual fruit body formation, pigment carotenoid production even secondary metabolites. Fungi adopted three groups photoreceptors: (I) blue receptors, White Collars, vivid, cryptochromes, F proteins DNA photolyases, (II) red sensors, phytochromes (III) green sensors microbial rhodopsins. Most mechanistic data were elucidated roles Collar Complex (WCC) fungal kingdom. The WCC acts as both photoreceptor transcription factor by binding target genes, whereas phytochrome initiates cascade using mitogen-activated protein kinases elicit its cellular responses. Although mechanism photoreception has been studied great detail, not compared vision. Therefore, this review will mainly focus findings derived from two model organisms, namely Aspergillus nidulans Neurospora crassa comparison some mechanisms Our be way is translated into changes gene expression, influences morphogenesis metabolism fungi.

Language: Английский

Citations

18
The KdmB-EcoA-RpdA-SntB (KERS) chromatin regulatory complex controls development, secondary metabolism and pathogenicity in Aspergillus flavus DOI Creative Commons
Betim Karahoda,

Brandon T. Pfannenstiel,

Özlem Sarikaya Bayram

et al.

Fungal Genetics and Biology, Journal Year: 2023, Volume and Issue: 169, P. 103836 - 103836

Published: Sept. 3, 2023

The filamentous fungus Aspergillus flavus is a plant and human pathogen predominantly found in the soil as spores or sclerotia capable of producing various secondary metabolites (SM) such carcinogenic mycotoxin aflatoxin. Recently, we have discovered novel nuclear chromatin binding complex (KERS) that contains JARID1-type histone demethylase KdmB, putative cohesion acetyl transferase EcoA, class I type deacetylase RpdA PHD ring finger reader protein SntB model nidulans. Here, show presence KERS A. by immunoprecipitation-coupled mass spectrometry constructed kdmBΔ rpdAΔ strains to study their roles fungal development, SM production post-translational modifications (HPTMs). We KdmB couple regulation gene clusters with light-responses HPTMs. opposing light-induced asexual conidiation, while both factors are positive regulators development through nsdC nsdD pathway. essential for productions aflatoxin (similar findings SntB) well cyclopiazonic acid, ditryptophenaline leporin B controlling respective biosynthetic clusters. further regulate H3K4me3 H3K9me3 levels, also acts on H3K14ac levels extracts. Therefore, modifiers key metabolism flavus.

Language: Английский

Citations

16
Genetic Regulation of Mycotoxin Biosynthesis DOI Creative Commons
Wenjie Wang, Xinle Liang, Yudong Li

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 9(1), P. 21 - 21

Published: Dec. 22, 2022

Mycotoxin contamination in food poses health hazards to humans. Current methods of controlling mycotoxins still have limitations and more effective approaches are needed. During the past decades years, variable environmental factors been tested for their influence on mycotoxin production leading elucidation a complex regulatory network involved biosynthesis. These regulators putative targets screening molecules that could inhibit synthesis. Here, we summarize mechanisms hierarchical regulators, including pathway-specific global epigenetic most critical (aflatoxins, patulin, citrinin, trichothecenes fumonisins). Future studies regulation will provide valuable knowledge exploring novel biosynthesis efficient way.

Language: Английский

Citations

20
SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus DOI Creative Commons
Dandan Wu, Chi Yang,

Yanfang Yao

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: March 4, 2024

The epigenetic reader SntB was identified as an important transcriptional regulator of growth, development, and secondary metabolite synthesis in Aspergillus flavus . However, the underlying molecular mechanism is still unclear. In this study, by gene deletion complementation, we found essential for mycelia conidial production, sclerotia formation, aflatoxin synthesis, host colonization. Chromatin immunoprecipitation sequencing (ChIP-seq) RNA (RNA-seq) analysis revealed that played key roles oxidative stress response A. , influencing related activity, especially catC encoding catalase. regulated expression activity with or without stress, to level secretory lipase (G4B84_008359). showed CatC participated regulation fungal morphogenesis, reactive oxygen species (ROS) level, significantly sensitive reaction AFB1 yield under stress. Our study potential machinery mycotoxin anabolism, virulence through axle from H3K36me3 modification biosynthesis. results shed light into SntB-mediated transcript pathways anabolism virulence, which provided strategy control contamination its aflatoxins.

Language: Английский

Citations

3
Locus-specific chromatin proteomics using dCas-guided proximity labelling in aspergillus nidulans DOI Creative Commons
Thomas Svoboda,

Dominik Niederdöckl-Loibl,

Andreas Schüller

et al.

Fungal Genetics and Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103973 - 103973

Published: March 1, 2025

Language: Английский

Citations

0
IDH1 regulates human erythropoiesis by eliciting chromatin state reprogramming DOI Open Access
Mengjia Li, Hengchao Zhang, Xiuyun Wu

et al.

Published: March 25, 2025

Isocitrate dehydrogenase 1 (IDH1) is the key enzyme that was involved in modulation of cellular metabolism, epigenetic modification and redox states homeostasis. Gain-of-function mutations decreased expression IDH1 have been demonstrated to be tightly associated with pathogenesis various myeloid malignancies characterized by ineffective erythropoiesis, such as acute leukemia (AML) myelodysplastic syndrome (MDS). However, function mechanism human erythropoiesis still remains further explored. Here, utilizing system, we present an evidence IDH1-mediated chromatin state reprogramming besides its well-characterized metabolism effects. We showed knockdown induced reorganization subsequently led abnormalities biological events erythroid precursors, which could not rescued addition reactive oxygen species (ROS) scavengers or supplementation α-ketoglutarate (α-KG). revealed deficiency induces genome-wide changes distribution intensity multiple histone marks, among H3K79me3 identified a critical factor reprogramming. Integrated analysis ChIP-seq, ATAC-seq RNA-seq recognized SIRT1 gene affected deficiency. Thus, our current work provided novel insights for clarifying fundamental has substantial implications in-depth understanding diseases dysfunction accordingly development therapeutic strategies.

Language: Английский

Citations

0
IDH1 regulates human erythropoiesis by eliciting chromatin state reprogramming DOI Creative Commons

Meng‐Jia Li,

Hengchao Zhang, Xiuyun Wu

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: April 29, 2025

Isocitrate dehydrogenase 1 (IDH1) is the key enzyme that can modulate cellular metabolism, epigenetic modification, and redox homeostasis. Gain-of-function mutations decreased expression of IDH1 have been demonstrated to be associated with pathogenesis various myeloid malignancies characterized by ineffective erythropoiesis, such as acute leukemia (AML) myelodysplastic syndrome (MDS). However, function mechanism in human erythropoiesis still remains unclear. Here, utilizing system, we present an evidence IDH1-mediated chromatin state reprogramming besides its well-characterized metabolism effects. We found knockdown induced reorganization subsequently led abnormalities biological events erythroid precursors, which could not rescued addition reactive oxygen species (ROS) scavengers or supplementation α-ketoglutarate (α-KG).We further revealed induces genome-wide changes distribution intensity multiple histone marks, among H3K79me3 was identified a critical factor reprogramming. Integrated analysis ChIP-seq, ATAC-seq, RNA-seq recognized SIRT1 gene affected deficiency. Thus, our current work provided novel insights for clarifying fundamental has substantial implications in-depth understanding diseases dysfunction accordingly development therapeutic strategies.

Language: Английский

Citations

0
Regulation of Histone Acetylation Modification on Biosynthesis of Secondary Metabolites in Fungi DOI Open Access

Xuwen Hou,

Liyao Liu,

Yu Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 25 - 25

Published: Dec. 24, 2024

The histone acetylation modification is a conservative post-translational epigenetic regulation in fungi. It includes and deacetylation at the lysine residues of histone, which are catalyzed by acetyltransferase (HAT) deacetylase (HDAC), respectively. plays crucial roles fungal growth development, environmental stress response, secondary metabolite (SM) biosynthesis, pathogenicity. One most important to regulate gene expression that responsible for SM biosynthesis This mini-review summarized HATs HDACs on SMs In cases, positively regulated SMs, while had their negative regulations. Some were revealed biosynthesis. Hda1 was found be efficient regulator affect species mainly from genera Aspergillus, Calcarisporium, Cladosporium, Fusarium, Monascus, Penicillium, Pestalotiopsis. With strategy modification, some harmful will inhibited, production useful bioactive promoted subsequent research should focus study regulatory mechanisms.

Language: Английский

Citations

2