The LOV-domain blue-light receptor LreA of the fungus Alternaria alternata binds predominantly FAD as chromophore and acts as a light and temperature sensor DOI Creative Commons

Lars Schuhmacher,

Steffen Heck,

Michael Pitz

et al.

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(5), P. 107238 - 107238

Published: March 28, 2024

Light and temperature sensing are important features of many organisms. may provide energy but also be used by non-photosynthetic organisms for orientation in the environment. Recent evidence suggests that plant fungal phytochrome phototropin serve dual functions as light sensors. Here we characterized LOV-domain blue-light receptor LreA Alternaria alternata show it predominantly contains FAD chromophore. Blue-light illumination induced ROS production followed protein agglomeration vitro. In vivo control activity. acts photoreceptor triggered temperature-shift gene expression. Both responses required conserved amino acid cysteine 421. We therefore propose mimics photoresponse, which could ancient function chromoprotein. Temperature-dependent expression with was distinct from response suggesting fine-tuned, photoreceptor-specific regulation.

Language: Английский

Photoregulation of the biosynthetic activity of the edible medicinal mushroom Lentinula edodes in vitro DOI
Оksana Mykchaylova, Halyna Dubova, A.М. Negriyko

et al.

Photochemical & Photobiological Sciences, Journal Year: 2024, Volume and Issue: 23(3), P. 435 - 449

Published: Jan. 30, 2024

Language: Английский

Citations

4
Influence of low-intensity artificial light on the fatty acid profile of the biotechnologically important culinary mushroom Pleurotus eryngii in vitro DOI Creative Commons
Оksana Mykchaylova,

Anatole Besarab,

A.М. Negriyko

et al.

BMC Biotechnology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 24, 2025

The problem of searching for environmentally friendly regulators the biosynthetic activity edible and medicinal mushrooms is crucial creating highly effective biotechnologies. One such regulator light. This study aimed to compare evaluate fatty acid profile fat quality indices lipids from mycelial mass Pleurotus eryngii under various light-emitting diode (LED) laser light irradiation regimes. To determine effect artificial on P. eryngii, an lighting system based LED matrices with wavelengths 470 nm (blue), 530 (green), 650 (red), as well argon gas a coherent visible source at 488 nm, was used. For all experimental variants, energy density surface samples set same, providing dose 240 mJ/cm2. Irradiation carried continuously. Twenty-seven acids were identified in studied samples, including nine saturated (SFAs), eight monounsaturated (MUFAs) ten polyunsaturated (PUFAs). control sample (without irradiation) contained lowest number (fourteen). With modes, decrease amount SFA formation new MUFA PUFA chain length C20-C24, which absent control, observed. Blue stimulated synthesis significant amounts α-linolenic (C18:3ω-3). ratios ΣPUFA/ΣSFA, ΣPUFA/ΣMUFA ΣPUFAω-6/ω-3 mycelium irradiated blue within optimal values human diet. selected photoactivation modes using low-intensity different spectral composition coherence may have potential biotechnology submerged cultivation obtain improved profile, can be considered useful lipids.

Language: Английский

Citations

0
Recent advances in carotenoid absorption, distribution, and elimination DOI Creative Commons

Wafa'a Hajeer,

Amparo Blanco,

Anthony P. Miller

et al.

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Journal Year: 2025, Volume and Issue: unknown, P. 159619 - 159619

Published: April 1, 2025

Carotenoids are a class of pigments with antioxidant properties synthesized by photosynthetic and heterotrophic organisms. Humans can store carotenoids in their intact form or cleave them enzymatically to apocarotenoids such as vitamin A, hormone-like nutrient crucial roles gene expression vision. Clinical preclinical studies suggest that the consumption diets rich attenuate cardiometabolic diseases, some types cancer, neurodegenerative disorders, inflammatory conditions. The bioactive depend, at least part, on accumulation target tissues. However, pathways drive carotenoid absorption, delivery, tissues remain largely uncharacterized. This review provides critical overview experimental models utilized monitor homeostasis mammals. We also delve into recent findings concerning intestinal uptake, bodily distribution, cellular intracellular trafficking. Finally, we discuss physiological relevance fecal elimination pathway operates independently enzymatic cleavage. Establishing players governing biodistribution is essential maximize humans prevent chronic diseases.

Language: Английский

Citations

0
Comparative Lipidomics and Growth Response of Flammulina velutipes Mycelium under Blue and White LED Light Spectra and Various Intensities DOI
J L Fan, Dongmei Wang, Shuhei Kaneko

et al.

ACS Agricultural Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Language: Английский

Citations

0
Experimental Evidence of Fungi Sensing and Communication DOI

Samuel Ojo,

Xiong Yu

Published: Jan. 1, 2025

Language: Английский

Citations

0
UV-C treatment has a higher efficacy on reducing germination of spores rather than mycelium growth DOI Creative Commons

Aileen Hahlbohm,

Christine Struck,

Friederike de Mol

et al.

European Journal of Plant Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Language: Английский

Citations

0
Integrated transcriptome and metabolome analysis provides insights into blue light response of Flammulina filiformis DOI Creative Commons
Huan Wang, Shuting Zhao,

Zhiyang Han

et al.

AMB Express, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 13, 2024

Abstract Blue light promotes primordium differentiation and fruiting body formation of mushroom. However, the blue response mechanism mushroom remains unclear. In this study, mycelium Flammulina filiformis was exposed to light, red dark conditions, then comparative metabolome transcriptome analysis applied explore metabolic regulation F. under conditions. The yield condition much higher than that Metabolome showed treatment reduced concentrations many low molecular weight carbohydrates in pilei, but it promoted accumulation some stipes. also decreased organic acids levels tyrosine tryptophan stipes, largely lysine organ. stipes , shifted metabolite flow synthesis through inhibiting aromatic amino acids, thereby enhancing its nutritional medicinal values. displayed enhanced downregulation methyltransferase gene L-lysine 6-monooxygenase gene. Additionally, upregulated hydrolase genes improve ability stipe biodegrade medium elevated growth rate body.

Language: Английский

Citations

2
Red and far-red light improve the antagonistic ability of Trichoderma guizhouense against phytopathogenic fungi by promoting phytochrome-dependent aerial hyphal growth DOI Creative Commons
Tingting Sun, Yifan Li, Jie Li

et al.

PLoS Genetics, Journal Year: 2024, Volume and Issue: 20(5), P. e1011282 - e1011282

Published: May 20, 2024

Light as a source of information regulates morphological and physiological processes fungi, including development, primary secondary metabolism, or the circadian rhythm. signaling in fungi depends on photoreceptors downstream components that amplify signal to govern expression an array genes. Here, we investigated effects red far-red light mycoparasite Trichoderma guizhouense its mycoparasitic potential. We show invasion strategy T . attacked species increased aerial hyphal growth led faster overgrowth colonies. Molecular experiments transcriptome analyses revealed are sensed by phytochrome FPH1 further transmitted MAPK HOG pathway bZIP transcription factor ATF1. Overexpression red- light-induced fluffy gene fluG dark resulted abundant hyphae formation thereby improvement antagonistic ability against phytopathogenic fungi. Hence, is important for interaction. The aggressiveness -overexpressing strains was phenocopied four random mutants obtained after UV mutagenesis. Therefore, appears be trait potential

Language: Английский

Citations

2
Effects of Light on the Fruiting Body Color and Differentially Expressed Genes in Flammulina velutipes DOI Creative Commons
Ji-Hoon Im,

Che-Hwon Park,

Ju-Hyeon Shin

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(6), P. 372 - 372

Published: May 22, 2024

Light plays vital roles in fungal growth, development, reproduction, and pigmentation. In Flammulina velutipes, the color of fruiting body exhibits distinct changes response to light; however, underlying molecular mechanisms remain unknown. Therefore, this study, we aimed analyze F. velutipes transcriptome under red, green, blue light-emitting diode (LED) lights identify key genes affecting light fungus. Additionally, conducted protein–protein interaction (PPI) network analysis previously reported color-related gene, Fvpal1, hub genes. Phenotypic revealed that bodies exposed green were darker than those untreated or red light, with intensifying more after 48 h exposure compared 24 exposure. Differentially expressed gene (DEG) analyses all treatments for numbers DEGs 17, 74, 257 lights, respectively. Subsequently, functional enrichment was identified which influenced velutipes. total, 103 168 downregulated included analysis. Among enriched both treatments, four related monooxygenases, three annotated as cytochrome P450s are crucial various metabolic processes fungi. PPI Fvpal1 associations 11 genes, among expression one pyridoxal-dependent decarboxylase, upregulated light. These findings contribute our understanding involved offer potential markers further exploration light-mediated regulatory pathways.

Language: Английский

Citations

2
Effects of light quality on agronomic traits, antioxidant capacity and nutritional composition of Sarcomyxa Edulis DOI Creative Commons
Tian Tian,

Hui-Yue Hu,

Yongsheng Ma

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 21, 2024

Sarcomyxa edulis, a notable edible and medicinal mushroom indigenous to northeast China, is celebrated for its high nutritional value delightful taste. In this study, white light as control examined the effects of red, green, blue on agronomic traits, antioxidant capabilities, composition S. edulis. The results showed that different monochromatic qualities affected color edulis pileus, with demonstrating particular efficacy. Furthermore, also regulated pileus length, whereas red was instrumental in significantly increasing stalk length. Regarding capacity, compared green light, activities POD, SOD, CAT were improved by irradiation, decreased MDA levels, free radical scavenging potential. Additionally, exposure led an increase contents 15 amino acids. Green treatment reduced crude fat content. For first time, we found quality key factor controlling Blue effective way regulate size improve properties. photobiological characteristics response environment clarified.

Language: Английский

Citations

2