Bone Marrow Stromal Cells Generate a Pro-Healing Inflammasome When Cultured on Titanium–Aluminum–Vanadium Surfaces with Microscale/Nanoscale Structural Features DOI Creative Commons
David J. Cohen,

Christine M. Van Duyn,

Jingyao Deng

et al.

Biomimetics, Journal Year: 2025, Volume and Issue: 10(1), P. 66 - 66

Published: Jan. 19, 2025

The surface topography and chemistry of titanium–aluminum–vanadium (Ti6Al4V) implants play critical roles in the osteoblast differentiation human bone marrow stromal cells (MSCs) creation an osteogenic microenvironment. To assess effects a microscale/nanoscale (MN) topography, this study compared MN-modified, anodized, smooth Ti6Al4V surfaces on MSC response, for first time, directly contrasted MN-induced with culture tissue polystyrene (TCPS) medium (OM). Surface characterization revealed distinct differences microroughness, composition, among substrates. MSCs MN exhibited enhanced osteoblastic differentiation, evidenced by increased expression RUNX2, SP7, BGLAP, BMP2, BMPR1A (fold increases: 3.2, 1.8, 1.4, 1.3, 1.2). also induced pro-healing inflammasome upregulation anti-inflammatory mediators (170–200% increase) downregulation pro-inflammatory factors (40–82% reduction). Integrin shifted towards osteoblast-associated integrins surfaces. RNA-seq analysis gene profiles between those OM, only 199 shared genes out over 1000 differentially expressed genes. Pathway showed that promoted formation, maturation, remodeling through non-canonical Wnt signaling, while OM stimulated endochondral development mineralization via canonical Wnt3a signaling. These findings highlight importance properties directing indicate MN-modified act signaling pathways differ from methods, more accurately mimicking peri-implant osteogenesis vivo.

Language: Английский

Transforming Clinical Research: The Power of High-Throughput Omics Integration DOI Creative Commons
Rui Vitorino

Proteomes, Journal Year: 2024, Volume and Issue: 12(3), P. 25 - 25

Published: Sept. 6, 2024

High-throughput omics technologies have dramatically changed biological research, providing unprecedented insights into the complexity of living systems. This review presents a comprehensive examination current landscape high-throughput pipelines, covering key technologies, data integration techniques and their diverse applications. It looks at advances in next-generation sequencing, mass spectrometry microarray platforms highlights contribution to volume precision. In addition, this critical role bioinformatics tools statistical methods managing large datasets generated by these technologies. By integrating multi-omics data, researchers can gain holistic understanding systems, leading identification new biomarkers therapeutic targets, particularly complex diseases such as cancer. The also electronic health records (EHRs) potential for cloud computing big analytics improve storage, analysis sharing. Despite significant advances, there are still challenges complexity, technical limitations ethical issues. Future directions include development more sophisticated computational application advanced machine learning techniques, which addressing heterogeneity datasets. aims serve valuable resource practitioners, highlighting transformative advancing personalized medicine improving clinical outcomes.

Language: Английский

Citations

20
Database resources of the National Center for Biotechnology Information in 2025 DOI Creative Commons
Eric W Sayers, Jeffrey Beck, Evan Bolton

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 53(D1), P. D20 - D29

Published: Nov. 11, 2024

The National Center for Biotechnology Information (NCBI) provides online information resources biology, including the GenBank® nucleic acid sequence repository and PubMed® of citations abstracts published in life science journals. NCBI search retrieval operations most these data from 31 distinct repositories knowledgebases. E-utilities serve as programming interface these. Resources receiving significant updates past year include PubMed, PubMed Central, Bookshelf, NIH Comparative Genomics Resource, BLAST, Sequence Read Archive, Taxonomy, iCn3D, Conserved Domain Database, Pathogen Detection, antimicrobial resistance PubChem. These can be accessed through home page at https://www.ncbi.nlm.nih.gov.

Language: Английский

Citations

20
The efflux pump ABCC1/MRP1 constitutively restricts PROTAC sensitivity in cancer cells DOI Creative Commons
Gernot Wolf, Conner Craigon, Shao Thing Teoh

et al.

Cell chemical biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Proteolysis targeting chimeras (PROTACs) are bifunctional molecules that induce selective protein degradation by linking an E3 ubiquitin ligase enzyme to a target protein. This approach allows scope for "undruggable" proteins, and several PROTACs have reached the stage of clinical candidates. However, roles cellular transmembrane transporters in PROTAC uptake efflux remain underexplored. Here, we utilized transporter-focused genetic screens identify ATP-binding cassette transporter ABCC1/MRP1 as key resistance factor. Unlike previously identified inducible exporter ABCB1/MDR1, ABCC1 is highly expressed among cancers various origins constitutively restricts bioavailability. Moreover, genome-wide screen, candidates involved processes such ubiquitination, mTOR signaling, apoptosis factors resistance. In summary, our findings reveal crucial active pump limiting efficacy cancer cells, offering insights overcoming drug

Language: Английский

Citations

4
The cell colony development is connected with the accumulation of embryogenesis-related proteins and dynamic distribution of cell wall components in in vitro cultures of Fagopyrum tataricum and Fagopyrum esculentum DOI Creative Commons
Magdalena Zaranek, Artur Piński, Bożena Skupień-Rabian

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 24, 2025

Language: Английский

Citations

1
16S rRNA sequencing reveals synergistic effects of silkworm feces and earthworms on soil microbial diversity and resilience under elevated temperatures DOI

Fuzhi Lu,

De‐Long Guan, Xiaoyan Zhang

et al.

Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 207, P. 105952 - 105952

Published: Feb. 10, 2025

Language: Английский

Citations

1
Evolution of sexual systems and regressive evolution in Riccia DOI Creative Commons
Jonathan Levins, Łukasz Paukszto, Katarzyna Krawczyk

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

The genus Riccia is the most speciose of Marchantiopsida, complex thalloid liverworts. In contrast to predicted ancestral liverwort and majority extant liverworts that are dioicous, species monoicous. Both gametophyte sporophyte generations have undergone regressive evolution in Riccia, with lacking many adaptations for spore dispersal. Phylogenetic reconstructions suggest multiple transitions between dioicy monoicy, vice versa, within Riccia. We analysed genomes two transcriptomes additional explore sexual systems investigate genomic signatures evolution. Genomic analyses a transition from monoicy at base clade subsequent reversion concomitant new sex chromosome, followed by further back monoicy. Additionally, exhibit significant losses transcription factors known control aspects development. Comparisons monotypic sister genus, Ricciocarpos, independent evolutions both reductive morphologies genera. morphology has facilitated their colonization extreme habitats but perhaps canalized them remain there.

Language: Английский

Citations

1
Applying the FAIR Principles to computational workflows DOI Creative Commons
Sean Wilkinson, Meznah Aloqalaa, Khalid Belhajjame

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Feb. 24, 2025

Recent trends within computational and data sciences show an increasing recognition adoption of workflows as tools for productivity reproducibility that also democratize access to platforms processing know-how. As digital objects be shared, discovered, reused, benefit from the FAIR principles, which stand Findable, Accessible, Interoperable, Reusable. The Workflows Community Initiative's Working Group (WCI-FW), a global open community researchers developers working with across disciplines domains, has systematically addressed application both software principles workflows. We present recommendations commentary reflects our discussions justifies choices adaptations. These are offered workflow users authors, management system developers, providers services guidelines fodder discussion. we propose in this paper will maximize their value research assets facilitate by wider community.

Language: Английский

Citations

1
A case for global standardisation of genomics and wastewater-based epidemiology DOI Creative Commons

Noemí Cajas-Corrales,

Diana Vallejo-Espín,

William Calero‐Cáceres

et al.

The Lancet Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 101092 - 101092

Published: March 1, 2025

Language: Английский

Citations

1
Bioactive Polyphenolic Compounds from Propolis of Tetragonula carbonaria in the Gibberagee Region, New South Wales, Australia DOI Creative Commons

Dylan W. Ebner,

D. L. Woods,

Trong D. Tran

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(6), P. 965 - 965

Published: March 12, 2025

Stingless bee propolis has emerged globally as a new source of bioactive molecules that can advance human health. However, limited research been conducted on Australian stingless propolis. This study investigated the chemical composition and biological activity produced by bees Tetragonula carbonaria from Gibberagee, distinct region New South Wales state in Australia. Using bioassay-guided fractionation, twelve compounds were isolated, including six A-ring methylated flavonoids. Nine these demonstrated strong scavenging against 2,2-diphenyl-1-picrylhydrazyl radicals, with five exhibiting greater potency than vitamin C. Chemical structures seven additional minor flavonoids determined through an intensive MS/MS data analysis. In silico screening 19 revealed all, except for gallic acid, displayed higher binding affinity to α-glucosidase antidiabetic drug, voglibose. showed Gibberagee is promising nutraceutical cosmeceutical applications owing its antioxidant properties. The unique profile C-methylated potentially provides valuable insights into botanical origin.

Language: Английский

Citations

1
Comparative genomics reveals genetic diversity and differential metabolic potentials of the species of Arachnia and suggests reclassification of Arachnia propionica E10012 (=NBRC_14587) as novel species DOI
R. Suresh,

Susanthika Jayachandiran,

Pratebha Balu

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(4)

Published: March 18, 2025

Language: Английский

Citations

1