MYB107 and MYB9 Homologs Regulate Suberin Deposition in Angiosperms

The Plant Cell, Journal Year: 2016, Volume and Issue: 28(9), P. 2097 - 2116

Published: Sept. 1, 2016

Suberin, a polymer composed of both aliphatic and aromatic domains, is deposited as rough matrix upon plant surface damage during normal growth in the root endodermis, bark, specialized organs (e.g., potato [Solanum tuberosum] tubers), seed coats. To identify genes associated with developmental control suberin deposition, we investigated chemical composition transcriptomes suberized tomato (Solanum lycopersicum) russet apple (Malus x domestica) fruit surfaces. Consequently, gene expression signature for assembly was revealed that highly conserved angiosperms. Seed permeability assays knockout mutants corresponding to regulatory proteins (i.e., AtMYB9 AtMYB107) required Arabidopsis thaliana coat. Seeds myb107 myb9 displayed significant reduction monomers altered levels other coat-associated metabolites. They also exhibited increased permeability, lower germination capacities under osmotic salt stress. AtMYB107 appear synchronize transcriptional induction monomer biosynthesis transport polymerization outer integument layer. Collectively, our findings establish system controlling developmentally suberin, which likely differs from one stress-induced recognized date.

Language: Английский

---

CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew

BMC Plant Biology, Journal Year: 2020, Volume and Issue: 20(1)

Published: June 19, 2020

Abstract Background The development of CRISPR/Cas9 technology has facilitated targeted mutagenesis in an efficient and precise way. Previously, RNAi silencing the susceptibility ( S ) gene P owdery M ildew R esistance 4 PMR4 tomato been shown to enhance resistance against powdery mildew pathogen Oidium neolycopersici On ). Results To study whether full knock-out would result a higher level than RNAi-silenced transgenic plants we generated CRISPR mutants. We used construct containing four single-guide RNAs (sgRNAs) targeting increase possibility large deletions After PCR-based selection sequencing transformants, identified five different mutation events, including from 900-bp, 1-bp insertion 892-bp inversion. These mutants all showed reduced based on visual scoring disease symptoms quantification relative fungal biomass. Histological observations revealed significantly occurrence hypersensitive response-like cell death at sites infection pmr4 compared wild-type plants. Both haustorial formation hyphal growth were diminished but not completely inhibited Conclusion CRISPR/Cas-9 resulted with complete loss PM On. Our demonstrates efficiency versatility system as powerful tool characterize -genes by generating types mutations.

Language: Английский

---

CRISPR/Cas9‐mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β‐1,2‐xylose and core α‐1,3‐fucose

Plant Biotechnology Journal, Journal Year: 2018, Volume and Issue: 17(2), P. 350 - 361

Published: July 3, 2018

Plants offer fast, flexible and easily scalable alternative platforms for the production of pharmaceutical proteins, but differences between plant mammalian N-linked glycans, including presence β-1,2-xylose core α-1,3-fucose residues in plants, can affect activity, potency immunogenicity plant-derived proteins. Nicotiana benthamiana is widely used transient expression recombinant proteins so it desirable to modify endogenous N-glycosylation machinery allow synthesis complex N-glycans lacking α-1,3-fucose. Here, we multiplex CRISPR/Cas9 genome editing generate N. lines deficient plant-specific α-1,3-fucosyltransferase β-1,2-xylosyltransferase reflecting mutation six different genes. We confirmed functional gene knockouts by Sanger sequencing mass spectrometry-based N-glycan analysis monoclonal antibody 2G12. Furthermore, compared CD64-binding affinity 2G12 glycovariants produced wild-type benthamiana, newly generated FX-KO line, Chinese hamster ovary (CHO) cells, confirming that glyco-engineered performed as well its CHO-produced counterpart.

Language: Английский

---

Mutation of a bHLH transcription factor allowed almond domestication

Science, Journal Year: 2019, Volume and Issue: 364(6445), P. 1095 - 1098

Published: June 13, 2019

How to make almonds palatable The domesticated almond tree has been feeding humans for millennia. Derivation from the wild, bitter, and toxic required loss of cyanogenic diglucoside amygdalin. Sánchez-Pérez et al. sequenced genome analyzed genomic region responsible this shift. key change turned out be a point mutation in transcription factor that regulates production P450 monooxygenases biosynthetic pathway compound. Science , issue p. 1095

Language: Английский

---

CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato against Tomato Yellow Leaf Curl Virus and Powdery Mildew

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(4), P. 1878 - 1878

Published: Feb. 13, 2021

Tomato is one of the major vegetable crops consumed worldwide. yellow leaf curl virus (TYLCV) and fungal Oidium sp. are devastating pathogens causing disease powdery mildew. Such viral reduce tomato crop yields cause substantial economic losses every year. Several commercial varieties include Ty-5 (SlPelo) Mildew resistance locus o 1 (SlMlo1) that carries susceptibility (S-gene) factors for TYLCV mildew, respectively. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) a valuable genome editing tool to develop disease-resistant varieties. In this regard, targeting encoded by host plant instead promising approach achieve pathogen without need stable inheritance CRISPR components. study, CRISPR/Cas9 system was employed target SlPelo SlMlo1 trait introgression in elite cultivar BN-86 confer host-mediated immunity against pathogens. SlPelo-knockout lines were successfully generated, carrying biallelic indel mutations. assays mutant confirmed suppressed accumulation restricted spread non-inoculated parts. Generated knockout showed complete mildew fungus. Overall, our results demonstrate efficiency introduce targeted mutagenesis rapid development pathogen-resistant tomato.

Language: Английский

---

Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress

The Plant Cell, Journal Year: 2022, Volume and Issue: 35(1), P. 67 - 108

Published: Aug. 26, 2022

We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these include the need better understand how plants detect water availability, temperature, salinity, rising carbon dioxide (CO2) levels; environmental signals interface endogenous signaling development (e.g. circadian clock flowering time); this integrated controls downstream responses stomatal regulation, proline metabolism, growth versus defense balance). The plasma membrane comes up frequently a site key transport events mechanosensing lipid-derived signaling, aquaporins). Adaptation extremes CO2 affects hydraulic architecture transpiration, well root shoot morphology, ways not fully understood. Environmental adaptation involves tradeoffs that limit ecological distribution crop resilience face changing increasingly unpredictable environments. Exploration diversity within among species can help us know which represent fundamental limits ones be circumvented bringing new trait combinations together. Better defining what constitutes beneficial resistance different contexts making connections between genes phenotypes, laboratory field observations, are overarching challenges.

Language: Английский

---

PRGdb 3.0: a comprehensive platform for prediction and analysis of plant disease resistance genes

Nucleic Acids Research, Journal Year: 2017, Volume and Issue: 46(D1), P. D1197 - D1201

Published: Oct. 25, 2017

The Plant Resistance Genes database (PRGdb; http://prgdb.org) has been redesigned with a new user interface, sections, tools and data for genetic improvement, allowing easy access not only to the plant science research community but also breeders who want improve disease resistance. home page offers an overview of easy-to-read search boxes that streamline queries directly show species which from candidate or cloned genes have collected. Bulk files curated resistance gene annotations are made available each hosted. Gene Model view detailed information on structure highlight shared attributes other genes. PRGdb 3.0 153 reference 177 072 annotated Pathogen Receptor (PRGs). Compared previous release, number putative increased 106 K 76 sequenced Viridiplantae algae genomes. DRAGO 2 tool, automatically annotates predicts (PRGs) DNA amino acid high accuracy sensitivity, added. BLAST implemented offer users opportunity annotate compare their own sequences. improved section diseases displays useful linked genomes connect complementary better address specific needs. Through, revised enlarged collection data, development renewed portal, engages in developing consensus plan knowledge strategies fight afflict main crops plants.

Language: Английский

---

Tomato R2R3-MYB Proteins SlANT1 and SlAN2: Same Protein Activity, Different Roles

PLoS ONE, Journal Year: 2015, Volume and Issue: 10(8), P. e0136365 - e0136365

Published: Aug. 26, 2015

Anthocyanins are water-soluble polyphenolic compounds with a high nutraceutical value. Despite the fact that cultivated tomato varieties do not accumulate anthocyanins in fruit, biosynthetic pathway can be activated vegetative organs by several environmental stimuli. Little is known about molecular mechanisms regulating anthocyanin synthesis tomato. Here, we carried out and functional characterization of two genes, SlAN2 SlANT1, encoding R2R3-MYB transcription factors. We show both induce ectopic transgenic lines, including fruit. However, only acts as positive regulator tissues under light or low temperature conditions.

Language: Английский

---

Genome sequences of horticultural plants: past, present, and future

Horticulture Research, Journal Year: 2019, Volume and Issue: 6(1)

Published: Oct. 7, 2019

Horticultural plants play various and critical roles for humans by providing fruits, vegetables, materials beverages, herbal medicines acting as ornamentals. They have also shaped human art, culture, environments thereby influenced the lifestyles of humans. With advent sequencing technologies, there has been a dramatic increase in number sequenced genomes horticultural plant species past decade. The are highly diverse complex, often with high degree heterozygosity ploidy due to their long complex history evolution domestication. Here we summarize advances genome plants, reconstruction pan-genomes, development databases. We discuss past, present, future studies related sequencing, data storage, quality, sharing, visualization provide practical guidance genomic plants. Finally, propose project well roadmap technical details toward three goals project.

Language: Английский

---

Genome-wide analysis of starch metabolism genes in potato (Solanum tuberosum L.)

BMC Genomics, Journal Year: 2017, Volume and Issue: 18(1)

Published: Jan. 5, 2017

Starch is the principle constituent of potato tubers and considerable importance for food non-food applications. Its metabolism has been subject extensive research over past decades. Despite its importance, a description complete inventory genes involved in starch their genome organization plants still missing. Moreover, mechanisms regulating expression leaves remain elusive with regard to differences between transitory storage metabolism, respectively. This study aimed at identifying mapping set genes, pattern using different sets transcriptome data. we wanted uncover transcription factors co-regulated accumulation order get insight into regulation metabolism. We identified 77 genomic loci encoding enzymes Novel isoforms many were found. Their analysis will help elucidate biosynthesis degradation. Expression led identification tissue-specific isoenzymes suggesting transcriptional leaf tuber tissues. Selection predominantly expressed developing exhibiting an indicative role enabled possible regulators by co-expression analysis. provides annotation metabolic localizations. Novel, so far undescribed, enzyme revealed. Comparative tuber- leaf-specific genes. finding suggests distinct regulatory Putative proteins have was verified quantitative RT-PCR.

Language: Английский

---