Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins DOI Creative Commons
Chanjae Lee, Rachael M. Cox, Ophelia Papoulas

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Dec. 2, 2020

Ciliary motility is driven by axonemal dyneins that are assembled in the cytoplasm before deployment to cilia. Motile ciliopathy can result from defects themselves or factors required for their cytoplasmic pre-assembly. Recent work demonstrates dyneins, specific assembly factors, and broadly-acting chaperones concentrated liquid-like organelles called DynAPs (Dynein Axonemal Particles). Here, we use

Language: Английский

ITGBL1 modulates integrin activity to promote cartilage formation and protect against arthritis DOI Open Access
Eun Kyung Song, Jimin Jeon, Dong Gil Jang

et al.

Science Translational Medicine, Journal Year: 2018, Volume and Issue: 10(462)

Published: Oct. 10, 2018

ITGBL1 promotes chondrogenesis and protects joint cartilage from osteoarthritis by inhibiting integrin-extracellular matrix interaction.

Language: Английский

Citations

50
Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support DOI Creative Commons
Marko E. Horb, Marcin Wlizla, Anita Abu‐Daya

et al.

Frontiers in Physiology, Journal Year: 2019, Volume and Issue: 10

Published: April 25, 2019

Two species of the clawed frog family, Xenopus laevis and tropicalis, are widely used as tools to investigate both normal disease-state biochemistry, genetics, cell biology developmental biology. To support specialist non-specialist scientists needing access these models for their research, a number centralised resources exist around world. These include centres that hold live frozen stocks transgenic, inbred mutant animals molecular resources. This infrastructure is supported by model organism database. Here we describe much this encourage community make best use it guide resource in developing new lines libraries.

Language: Английский

Citations

50
Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation DOI Creative Commons
Kitt Paraiso, Ira L. Blitz,

Masani Coley

et al.

Cell Reports, Journal Year: 2019, Volume and Issue: 27(10), P. 2962 - 2977.e5

Published: June 1, 2019

Language: Английский

Citations

47
An RNA-Seq Protocol for Differential Expression Analysis DOI Open Access
Nick Owens, Elena De Domenico, Michael J. Gilchrist

et al.

Cold Spring Harbor Protocols, Journal Year: 2019, Volume and Issue: 2019(6), P. pdb.prot098368 - pdb.prot098368

Published: April 5, 2019

Here we consider RNA-Seq, used to measure global gene expression through RNA fragmentation, capture, sequencing, and subsequent computational analysis. Xenopus, with its large number of RNA-rich, synchronously developing, accessible embryos, is an excellent model organism for exploiting the power high-throughput sequencing understand during development. present a standard RNA-Seq protocol performing two-state differential analysis (between groups replicates control treated embryos) using Illumina sequencing. Samples contain multiple whole polyadenylated mRNA measured under relative normalization. The divided into two parts: wet-lab processes prepare samples downstream including quality control, quantification expression, expression.

Language: Английский

Citations

46
Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins DOI Creative Commons
Chanjae Lee, Rachael M. Cox, Ophelia Papoulas

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Dec. 2, 2020

Ciliary motility is driven by axonemal dyneins that are assembled in the cytoplasm before deployment to cilia. Motile ciliopathy can result from defects themselves or factors required for their cytoplasmic pre-assembly. Recent work demonstrates dyneins, specific assembly factors, and broadly-acting chaperones concentrated liquid-like organelles called DynAPs (Dynein Axonemal Particles). Here, we use

Language: Английский

Citations

42