Antifungal Natural Products Originating from Endophytic and Rhizospheric Microbes Isolated from Coastal Vegetation DOI Creative Commons
Sumali Lakmini Dissanayake Jayaweera, Thi Thu Hao Van, Daniel A. Dias

et al.

Journal of Xenobiotics, Journal Year: 2025, Volume and Issue: 15(1), P. 32 - 32

Published: Feb. 17, 2025

Candida infections severely impact patients who are immunocompromised. Currently, there limited options to treat fungal infections, especially drug-resistant-fungal infections. Therefore, investigating alternative or repurposed antifungals is paramount. Endophytic microbes (EMs) and rhizospheric (RMs) emerge as promising reservoirs of bioactive natural compounds. Interestingly, plants that have adapted various environmental conditions harbour a plethora producing variety products can be assessed for potential antifungal activity. To date, EMs RMs residing in coastal their associated not been extensively studied reviewed. this comprehensive review will focus on products, extracted from coastal-vegetation-associated microbiota draw the attention research field. A literature search was conducted by examining both Scopus Google Scholar databases during period 2013–2024 related following vegetation: mangroves, sand dune plants, salt marsh seagrasses. 65 novel compounds derived coastal-plant identified. Mangroves were found most prominent host harbouring antifungal-producing compared with other plants. Coastal-plant-associated partners producers bacterial counterparts. Fifty-four fungal-EM/RM reported demonstrate activities against plant pathogenic fungi well human pathogens. Most bacterial-derived (11 antifungals) previously activity albicans.

Language: Английский

The changing epidemiology of fungal infections DOI Creative Commons
Cornelia Lass‐Flörl, Stephan Steixner

Molecular Aspects of Medicine, Journal Year: 2023, Volume and Issue: 94, P. 101215 - 101215

Published: Oct. 5, 2023

Invasive fungal diseases are common complications in critically ill patients and those with significant underlying imbalanced immune systems. Fungal co-, and/or super-infections emerging have become a rising concern within the last few years. In Europe, cases of candidiasis aspergillosis dominate, followed by mucormycosis India. Epidemiological studies show an increasing trend incidence all three entities. Parallel to this, shift pathogens is observed. More non-albicans Candida infections cryptic species on rise; may cover intrinsic resistance azoles other antifungal drugs. The recent COVID-19 pandemic led significantly invasive among hospitalized patients.

Language: Английский

Citations

49
Phylogenomic analysis of the Candida auris- Candida haemuli clade and related taxa in the Metschnikowiaceae, and proposal of thirteen new genera, fifty-five new combinations and nine new species DOI
Fei Liu,

Z-D Hu,

X-M Zhao

et al.

Persoonia - Molecular Phylogeny and Evolution of Fungi, Journal Year: 2024, Volume and Issue: 52(1), P. 22 - 43

Published: April 7, 2024

is a polyphyletic genus of asexually reproducing yeasts in the

Language: Английский

Citations

20
Global guideline for the diagnosis and management of candidiasis: an initiative of the ECMM in cooperation with ISHAM and ASM DOI Creative Commons
Oliver A. Cornely, Rosanne Sprute, Matteo Bassetti

et al.

The Lancet Infectious Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

19
A conceptual framework for nomenclatural stability and validity of medically important fungi: a proposed global consensus guideline for fungal name changes supported by ABP, ASM, CLSI, ECMM, ESCMID-EFISG, EUCAST-AFST, FDLC, IDSA, ISHAM, MMSA, and MSGERC DOI Creative Commons
Sybren de Hoog, Thomas J. Walsh, Sarah Ahmed

et al.

Journal of Clinical Microbiology, Journal Year: 2023, Volume and Issue: 61(11)

Published: Oct. 26, 2023

The rapid pace of name changes medically important fungi is creating challenges for clinical laboratories and clinicians involved in patient care. We describe two sources change which have different drivers, at the species versus genus level. Some suggestions are made here to reduce number changes. urge taxonomists provide diagnostic markers taxonomic novelties. Given instability phylogenetic trees due variable taxon sampling, we advocate maintain genera largest possible size. Reporting identified complexes or series should where comprise both overarching that molecular sibling, often cryptic species. Because use names same will be unavoidable many years come, an open access online database all fungi, with proper nomenclatural designation synonymy, essential. further recommend while discovery continues, adaptation new by reviewed routinely a standing committee validation stability over time, reference database, wherein reasons listed transparent way.

Language: Английский

Citations

33
Secreted Aspartic Proteinases: Key Factors in Candida Infections and Host-Pathogen Interactions DOI Open Access
Grażyna Braś, Dorota Satała, Magdalena Juszczak

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4775 - 4775

Published: April 27, 2024

Extracellular proteases are key factors contributing to the virulence of pathogenic fungi from genus Candida. Their proteolytic activities crucial for extracting nutrients external environment, degrading host defenses, and destabilizing internal balance human organism. Currently, enzymes most frequently described in this context secreted aspartic (Saps). This review comprehensively explores multifaceted roles Saps, highlighting their importance biofilm formation, tissue invasion through degradation extracellular matrix proteins components coagulation cascade, modulation immune responses via impairment neutrophil monocyte/macrophage functions, contribution antifungal resistance. Additionally, diagnostic challenges associated with Candida infections potential Saps as biomarkers were discussed. Furthermore, we examined prospects developing vaccines based on use protease inhibitors adjunctive therapies candidiasis. Given complex biology central role pathogenicity, a multidisciplinary approach may pave way innovative strategies open new opportunities clinical interventions against

Language: Английский

Citations

15
Nucleic-Acid-Based Molecular Fungal Diagnostics: A Way to a Better Future DOI Creative Commons

Rajendra Gudisa,

Ritika Harchand, Shivaprakash M. Rudramurthy

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(5), P. 520 - 520

Published: Feb. 29, 2024

The world has seen a tremendous increase in the number of fungal infections during past two decades. Recently, World Health Organisation released pathogen priority list for infections, signifying importance these fields research and public health. Microbiology laboratories demand an upgrade diagnostic system to keep up with increased burden infections. Diagnosis using conventional techniques always faced limitations terms specificity, sensitivity, turnaround time. Although methods are core pillars diagnosis, there is need molecular approaches. Molecular have revolutionised field diagnostics. diverse array techniques, including like Polymerase Chain Reaction (PCR), emerged as cornerstone transformed diagnostics, providing powerful tools rapid accurate identification pathogens. As technologies continue evolve, their integration into routine clinical practice holds promise improving patient outcomes through timely targeted antifungal interventions. This review will cover approaches involved moving from basic advanced-level nucleic-acid-based high throughput decreased time diagnosis serious

Language: Английский

Citations

12
Re-estimation of the burden of serious fungal diseases in Uganda DOI Creative Commons
Felix Bongomin, Richard Kwizera, Martha Namusobya

et al.

Therapeutic Advances in Infectious Disease, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 1, 2024

It is of utmost importance to monitor any change in the epidemiology fungal diseases that may arise from a number at-risk population or availability local data.

Language: Английский

Citations

11
Identification and antifungal susceptibility patterns of reference yeast strains to novel and conventional agents: a comparative study using CLSI, EUCAST and Sensititre YeastOne methods DOI Creative Commons
Andrés Ceballos-Garzón, Marion Holzapfel,

Jo Anne Welsch

et al.

JAC-Antimicrobial Resistance, Journal Year: 2025, Volume and Issue: 7(2)

Published: March 4, 2025

The aim of this study was to identify and determine the MICs 13 antifungal drugs, including novel agents ibrexafungerp, manogepix rezafungin, against 22 laboratory reference strains from 14 different Candida spp. allied yeast genera using EUCAST, CLSI Sensititre™ YeastOne™ (SYO) methods. Complete agreement between molecular proteomics methods observed for identification. compounds with greatest in vitro activity, as indicated by lowest geometric mean MIC (GM), were (GM: 0.01), isavuconazole 0.05) rezafungin 0.03-0.07). overall essential (EA) (within ±0 ±2 2-fold dilutions) methods, EUCAST CLSI, 95%, results ranging 82% (ibrexafungerp) 100% (amphotericin B, anidulafungin, fluconazole, 5-flucytosine micafungin). Regarding EA compared SYO, values 91% 89%, respectively. Nevertheless, when transformed into log2, significant differences (e.g. ibrexafungerp 5-flucytosine). At species level, Candidozyma auris duobushaemulonii exhibited highest number cases comparing three techniques each antifungal. high reinforces reliability SYO guiding therapy. However, EA, particularly 5-flucytosine, highlight importance continued evaluation these methodologies ensure consistency. Given that susceptibility testing plays a critical role treatment decisions, understanding variations is prevent potential misclassification profiles, which could impact clinical outcomes.

Language: Английский

Citations

2
Host cell responses to Candida albicans biofilm-derived extracellular vesicles DOI Creative Commons
Kamila Kulig, Ewelina Wronowska, Magdalena Juszczak

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 14, 2025

Candida albicans is a prevalent fungal pathogen responsible for infections in humans. As described recently, nanometer-sized extracellular vesicles (EVs) produced by C. play crucial role the pathogenesis of infection facilitating host inflammatory responses and intercellular communication. This study investigates functional properties EVs released biofilms formed two strains—3147 (ATCC 10231) SC5314—in eliciting responses. We demonstrate capability to trigger reactions human epithelial immune cells. The involvement was evidenced from initial stages infection, specifically adherence further established capacity these induce cytokine production A549 cell line, THP-1 macrophage-like cells, blood-derived monocytes differentiated into macrophages. Internalization cells confirmed, identifying macropinocytosis phagocytosis as most probable mechanisms, demonstrated using various inhibitors that target potential vesicle uptake pathways Additionally, their cargo were identified chemoattractants neutrophils. After verification vivo effect biofilm-derived on host, Galleria mellonella larvae an alternative model, it SC5314 increased mortality injected larvae. In conclusion, both types predominantly pro-inflammatory observed, highlighting significant response during infection.

Language: Английский

Citations

1
An update on current and novel molecular diagnostics for the diagnosis of invasive fungal infections DOI
Jeffrey D. Jenks, P. Lewis White, Sarah Kidd

et al.

Expert Review of Molecular Diagnostics, Journal Year: 2023, Volume and Issue: 23(12), P. 1135 - 1152

Published: Oct. 6, 2023

Invasive fungal infections cause millions of annually, but diagnosis remains challenging. There is an increased need for low-cost, easy to use, highly sensitive and specific molecular assays that can differentiate between colonized pathogenic organisms from different clinical specimens.

Language: Английский

Citations

22