Long noncoding RNAs contribute to DNA damage resistance in Arabidopsis thaliana

Genetics, Journal Year: 2023, Volume and Issue: 225(1)

Published: July 19, 2023

Abstract Efficient repair of DNA lesions is essential for the faithful transmission genetic information between somatic cells and genome integrity across generations. Plants have multiple, partially redundant, overlapping pathways, probably due to less constricted germline inevitable exposure light including higher energy wavelengths. Many proteins involved in their mode actions are well described. In contrast, a role damage-associated RNA components, evident from many other organisms, understood. Here, we challenged young Arabidopsis thaliana plants with two different types genotoxic stress performed de novo assembly transcriptome analysis. We identified three long noncoding RNAs (lncRNAs) that lowly or not expressed under regular conditions but up-regulated induced by damage. generated CRISPR/Cas deletion mutants found absence lncRNAs impairs recovery capacity stress. The loci highly conserved among world-wide distributed accessions within related species Brassicaceae group. Together, these results suggest function connection damage provide basis mechanistic analysis role.

Language: Английский

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The long noncoding RNA family gets bigger: thousands of new loci identified in naturalArabidopsisaccessions

The Plant Cell, Journal Year: 2023, Volume and Issue: 36(1), P. 6 - 7

Published: Sept. 19, 2023

Long noncoding RNAs (lncRNAs) are a class of RNA molecules that longer than 200 nucleotides and not translated into proteins.lncRNAs play important regulatory roles in various cellular processes, such as gene expression epigenetic regulation.They contribute to the complexity regulation involved shaping an organism's development, function, response its environment (Mattick et al., 2023).In plants, functions about two dozen lncRNAs have been elucidated (Palos 2023), but there is little doubt these cases only represent tip iceberg.

Language: Английский

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Transposable elements are prevalent vectors of transgenerational epigenetic inheritance in nature

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Loss of DNA methylation over transposable elements (TEs) can affect neighboring genes and be epigenetically inherited in plants, yet the determinants significance this additional system inheritance are unknown. Here, we demonstrate at thousands TE loci across Arabidopsis thaliana genome, that experimentally-induced hypomethylation transmitted transgenerationally reveal role small RNAs derived from related copies counteracting transmission. Using data >700 strains collected worldwide, uncover natural hundreds same loci, often situated near stress-responsive genes. Like their experimental counterparts, most epivariants tested without sequence changes therefore bona fide epialleles, although genetic factors modulate recurrence or persistence. Crucially, TE-mediated epiallelic variation associated with differential gene expression is generally causal may target selection specific environments, thus establishing its importance nature.

Language: Английский

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From Single nc-RNAs to Networks: Understanding the Complexity of Environmental Adaption

Published: Jan. 1, 2024

Language: Английский

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Meeting report: transposable elements at the crossroads of evolution, health and disease 2023

Mobile DNA, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 27, 2023

Language: Английский

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