Physiologia Plantarum,
Journal Year:
2024,
Volume and Issue:
177(1)
Published: Dec. 27, 2024
Abstract
The
classic
plant
growth‐promoting
phytohormone
cytokinin
has
been
identified
and
established
as
a
mediator
of
pathogen
resistance
in
different
species.
However,
the
effect
structurally
cytokinins
appears
to
vary
may
regulate
diverse
mechanisms
establish
resistance.
Hence,
we
comparatively
analysed
impact
six
adenine‐
phenylurea‐type
on
well‐established
pathosystem
Nicotiana
tabacum
‐
Pseudomonas
syringae
.
efficiency
effects
was
evaluated
based
impacts
host
defence
response
by
scoring
infection
symptoms
direct
assessment
proliferation
planta
To
identify
common
cytokinin‐specific
components
involved
effects,
transcriptome
profiling
targeted
metabolomics
were
conducted
leaves
treated
with
cytokinins.
We
observed
clearly
potentials
tested
either
suppressing
or
proliferation.
Gene
regulation
metabolite
analyses
revealed
cytokinin‐type
specific
components,
such
salicylic
acid
related
signalling,
expression
PR
proteins,
specialised
metabolism.
Cytokinins
also
strongly
affected
cell
physiological
parameters,
remarkable
decrease
amino
pools.
this
study
provides
comparative
information
mediating
one
well‐studied
insights
into
mediated
molecules.
This
is
particularly
relevant
for
studies
function
other
phytohormones
compounds
interacting
activities
context
infections
stress
scenarios,
considering
present
plants.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 10, 2024
ABSTRACT
Plant
immunity
activation
often
results
in
suppression
of
plant
growth,
particularly
the
case
constitutive
immune
activation.
We
discovered
that
signaling
phytohormone
cytokinin
(CK),
known
to
regulate
growth
through
control
cell
division
and
shoot
apical
meristem
(SAM)
activity,
can
be
suppressed
by
negative
crosstalk
with
defense
phytohormones
jasmonic
acid
(JA),
most
evidently,
salicylic
(SA).
show
changing
SA
on
CK
autoimmunity
mutants
targeted
increase
endogenous
levels
plants
resistant
pathogens
from
diverse
lifestyles,
relieves
reproductive
growth.
Moreover,
such
changes
result
a
novel
phenotype,
suggesting
role
for
SAM,
likely
regulation
nitrogen
response
cellular
redox
status.
Our
data
suggest
engineering
used
achieve
increased
pathogen
resistance.
SIGNIFICANCE
STATEMENT
Plants
constantly
integrate
environmental
stimuli
developmental
programs
optimize
their
fitness.
Excessive
system
leads
decreased
process
as
growth-defense
tradeoff.
Here,
we
adapted
Arabidopsis
tissues
change
phytohormonal
diminish
tradeoff,
resulting
broad
resistance
suppression.
Similar
approaches
could
different
contexts
outcomes
higher
stress
resilience
yield.
Biochemistry and Biophysics Reports,
Journal Year:
2024,
Volume and Issue:
39, P. 101756 - 101756
Published: June 16, 2024
Lonely
guy
(LOG)
proteins
are
phosphoribohydrolases
(PRHs)
that
key
cytokinin
(CK)-activating
enzymes
in
plant
and
non-plant
CK-producing
organisms.
During
CK
biosynthesis,
LOGs
catalyze
the
conversion
of
precursor
CK-nucleotides
(CK-NTs)
to
biologically
active
free
base
forms.
LOG/PRH
activity
has
been
detected
bacteria,
archaea,
algae,
fungi.
However,
these
organisms,
for
CK-NTs
non-CK-NTs
(e.g.,
adenine-NTs)
not
assessed
simultaneously,
which
leaves
limited
knowledge
about
substrate
specificity
LOGs.
Thus,
we
performed
bioinformatic
analyses
a
biochemical
characterization
LOG
ortholog
from
Stress Biology,
Journal Year:
2024,
Volume and Issue:
4(1)
Published: Aug. 13, 2024
Abstract
Cytokinin
oxidase/dehydrogenase
(CKX)
is
the
key
enzyme
that
has
been
observed
to
catalyze
irreversible
inactivation
of
cytokinins
and
thus
modulate
cytokinin
levels
in
plants.
CKX
gene
family
known
have
few
members
which
are,
expanded
genome
mainly
due
duplication
events.
A
total
nine
MiCKXs
were
identified
Morus
indica
cv
K2
with
almost
similar
structures
conserved
motifs
domains.
The
cis-
elements
along
expression
analysis
these
revealed
their
contrasting
specific
role
plant
development
across
different
developmental
stages.
localization
enzymes
ER
Golgi
bodies
signifies
functional
specification
property
getting
modified
post-translationally
carry
out
activities.
overexpression
MiCKX4
,
an
ortholog
AtCKX4
displayed
longer
primary
root
higher
number
lateral
roots.
Under
ABA
stress
also
transgenic
lines
showed
roots
tolerance
against
drought
as
compared
wild-type
In
this
study,
analyzed
bioinformatically
for
roles
under
abiotic
stresses.
Physiologia Plantarum,
Journal Year:
2024,
Volume and Issue:
177(1)
Published: Dec. 27, 2024
Abstract
The
classic
plant
growth‐promoting
phytohormone
cytokinin
has
been
identified
and
established
as
a
mediator
of
pathogen
resistance
in
different
species.
However,
the
effect
structurally
cytokinins
appears
to
vary
may
regulate
diverse
mechanisms
establish
resistance.
Hence,
we
comparatively
analysed
impact
six
adenine‐
phenylurea‐type
on
well‐established
pathosystem
Nicotiana
tabacum
‐
Pseudomonas
syringae
.
efficiency
effects
was
evaluated
based
impacts
host
defence
response
by
scoring
infection
symptoms
direct
assessment
proliferation
planta
To
identify
common
cytokinin‐specific
components
involved
effects,
transcriptome
profiling
targeted
metabolomics
were
conducted
leaves
treated
with
cytokinins.
We
observed
clearly
potentials
tested
either
suppressing
or
proliferation.
Gene
regulation
metabolite
analyses
revealed
cytokinin‐type
specific
components,
such
salicylic
acid
related
signalling,
expression
PR
proteins,
specialised
metabolism.
Cytokinins
also
strongly
affected
cell
physiological
parameters,
remarkable
decrease
amino
pools.
this
study
provides
comparative
information
mediating
one
well‐studied
insights
into
mediated
molecules.
This
is
particularly
relevant
for
studies
function
other
phytohormones
compounds
interacting
activities
context
infections
stress
scenarios,
considering
present
plants.
