Graft incompatibility between pepper and tomato can be attributed to genetic incompatibility between diverged immune systems DOI Creative Commons
Hannah Rae Thomas, Alice Gevorgyan,

Alexandra Hermanson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Summary Graft compatibility is the capacity of two plants to form cohesive vascular connections. Tomato and pepper are incompatible graft partners; however, underlying cause rejection between these species remains unknown. We diagnosed incompatibility tomato diverse varieties based on weakened biophysical stability, decreased growth, persistent cell death using trypan blue TUNEL assays. Transcriptomic analysis in junction was performed RNA-sequencing, molecular signatures for response were characterized meta-transcriptomic comparisons with other biotic processes. show that broadly cultivars. These partners activate prolonged transcriptional changes highly enriched defense Amongst processes broad NLR upregulation hypersensitive response. Using transcriptomic datasets a variety stress treatments, we identified significant overlap genetic profile grafting plant parasitism. In addition, found over 1000 genes uniquely upregulated grafts. Based overactivity, DNA damage, have determined likely caused by incompatibility, which triggers hyperimmune-response.

Language: Английский

Oligomerization-mediated autoinhibition and cofactor binding of a plant NLR DOI Creative Commons
Ma ShouCai, Chunpeng An, Aaron W. Lawson

et al.

Nature, Journal Year: 2024, Volume and Issue: 632(8026), P. 869 - 876

Published: June 12, 2024

Abstract Nucleotide-binding leucine-rich repeat (NLR) proteins play a pivotal role in plant immunity by recognizing pathogen effectors 1,2 . Maintaining balanced immune response is crucial, as excessive NLR expression can lead to unintended autoimmunity 3,4 Unlike most NLRs, the required for cell death 2 (NRC2) belongs small group characterized constitutively high without self-activation 5 The mechanisms underlying NRC2 autoinhibition and activation are not yet understood. Here we show that Solanum lycopersicum (tomato) ( Sl NRC2) forms dimers tetramers higher-order oligomers at elevated concentrations. Cryo-electron microscopy shows an inactive conformation of these oligomers. Dimerization oligomerization only stabilize state but also sequester from assembling into active form. Mutations dimeric or interdimeric interfaces enhance pathogen-induced Nicotiana benthamiana cryo-electron structures unexpectedly inositol hexakisphosphate (IP 6 ) pentakisphosphate bound inner surface C-terminal domain NRC2, confirmed mass spectrometry. phosphate-binding site impair phosphate binding NRC2-mediated N. Our study indicates negative regulatory mechanism suggests phosphates cofactors NRCs.

Language: Английский

Citations

22
Plant HEM1 specifies a condensation domain to control immune gene translation DOI
Yulu Zhou,

Ruixia Niu,

Zhijuan Tang

et al.

Nature Plants, Journal Year: 2023, Volume and Issue: 9(2), P. 289 - 301

Published: Feb. 16, 2023

Language: Английский

Citations

28
Progress in Plant Nitric Oxide Studies: Implications for Phytopathology and Plant Protection DOI Open Access
Michaela Sedlářová,

Tereza Jedelská,

A. Lebeda

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2087 - 2087

Published: Feb. 27, 2025

Nitric oxide (NO) is a gaseous free radical known to modulate plant metabolism through crosstalk with phytohormones (especially ABA, SA, JA, and ethylene) other signaling molecules (ROS, H2S, melatonin), regulate gene expression (by influencing DNA methylation histone acetylation) as well protein function post-translational modifications (cysteine S-nitrosation, metal nitrosation, tyrosine nitration, nitroalkylation). Recently, NO has gained attention molecule promoting crop resistance stress conditions. Herein, we review innovations from the field nanotechnology on an up-to-date phytopathological background.

Language: Английский

Citations

1
Combination of PAMP‐induced peptide signaling and its regulator SpWRKY65 boosts tomato resistance to Phytophthora infestans DOI

Ruirui Yang,

Chenglin Su,

Zhiyuan Xue

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)

Published: March 1, 2025

SUMMARY Late blight, caused by Phytophthora infestans ( P. ), seriously compromises tomato growth and yield. PAMP‐induced peptides (PIPs) are secreted that act as endogenous elicitors, triggering plant immune responses. Our previous research indicated the exogenous application of PIP1 from Solanum pimpinelifolium L3708, named SpPIP1, enhances resistance to . However, little is known about roles additional family members in In addition, there remains a significant gap understanding receptors SpPIPs transcription factors (TFs) regulate signaling defense, combination TFs defending against pathogens rarely studied. This study demonstrates SpPIP‐LIKE1 (SpPIPL1) also strengthens affecting phenylpropanoid biosynthesis pathway. Both SpPIP1 SpPIPL1 trigger defense responses manner dependent on RLK7L. Tomato plants overexpressing precursors (SpprePIP1 SpprePIPL1) exhibited enhanced expression pathogenesis‐related genes, elevated H 2 O ABA levels, increased lignin accumulation. Notably, SpWRKY65 was identified transcriptional activator SpprePIP1 SpprePIPL1 Disease assays gene analyses revealed overexpression (OEWRKY65) confers , while wrky65 knockout led opposite effect. Intriguingly, transgenic studies showed either spraying OEWRKY65 with or co‐overexpressing further augmented resistance, underscoring potential stacking enhancing disease resistance. summary, this offers new perspectives controlling late blight developing varieties improved The results emphasize an eco‐friendly strategy for crop protection, laying theoretical foundation advancing breeding.

Language: Английский

Citations

1
Concerted actions of PRR- and NLR-mediated immunity DOI
Jack Rhodes, Cyril Zipfel, Jonathan D. G. Jones

et al.

Essays in Biochemistry, Journal Year: 2022, Volume and Issue: 66(5), P. 501 - 511

Published: June 28, 2022

Plants utilise cell-surface immune receptors (functioning as pattern recognition receptors, PRRs) and intracellular nucleotide-binding leucine-rich repeat (NLRs) to detect pathogens. Perception of pathogens by these activates signalling resistance infections. PRR- NLR-mediated immunity have primarily been considered parallel processes contributing disease resistance. Recent studies suggest that two pathways are interdependent converge at multiple nodes. This review summarises provides a perspective on convergent points.

Language: Английский

Citations

30
Climate change reshaping plant-fungal interaction DOI
Abdul Waheed,

Yakoopjan Haxim,

Waqar Islam

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 238, P. 117282 - 117282

Published: Sept. 30, 2023

Language: Английский

Citations

17
The NLR immune receptor ADR1 and lipase-like proteins EDS1 and PAD4 mediate stomatal immunity in Nicotiana benthamiana and Arabidopsis DOI
Hanling Wang, Susheng Song, Shang Gao

et al.

The Plant Cell, Journal Year: 2023, Volume and Issue: 36(2), P. 427 - 446

Published: Oct. 18, 2023

Abstract In the presence of pathogenic bacteria, plants close their stomata to prevent pathogen entry. Intracellular nucleotide-binding leucine-rich repeat (NLR) immune receptors recognize effectors and activate effector-triggered responses. However, regulatory molecular mechanisms stomatal immunity involving NLR are unknown. Here, we show that Nicotiana benthamiana RPW8-NLR central receptor ACTIVATED DISEASE RESISTANCE 1 (NbADR1), together with key proteins ENHANCED SUSCEPTIBILITY (NbEDS1) PHYTOALEXIN DEFICIENT 4 (NbPAD4), plays an essential role in bacterial pathogen- flg22-induced by regulating expression salicylic acid (SA) abscisic (ABA) biosynthesis or response-related genes. NbADR1 recruits NbEDS1 NbPAD4 form a response complex. The transcription factor NbWRKY40e, association NbPAD4, modulates SA ABA genes influence immunity. NbADR1, NbEDS1, required for infection-enhanced binding NbWRKY40e ISOCHORISMATE SYNTHASE promoter. Moreover, ADR1-EDS1-PAD4 module regulates Arabidopsis (Arabidopsis thaliana). Collectively, our findings pivotal core intracellular immunity, which enables limit

Language: Английский

Citations

15
An Overview of PRR- and NLR-Mediated Immunities: Conserved Signaling Components across the Plant Kingdom That Communicate Both Pathways DOI Open Access
Candy Y. Ramírez-Zavaleta, Laura J. García‐Barrera, Lizette Liliana Rodríguez‐Verástegui

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 12974 - 12974

Published: Oct. 26, 2022

Cell-surface-localized pattern recognition receptors (PRRs) and intracellular nucleotide-binding domain leucine-rich repeat (NLRs) are plant immune proteins that trigger an orchestrated downstream signaling in response to molecules of microbial origin or host origin. Historically, PRRs have been associated with pattern-triggered immunity (PTI), whereas NLRs involved effector-triggered (ETI). However, recent studies reveal such binary distinction is far from being applicable the real world. Although perception pathogens final mounting achieved by different means, central hubs shared between PTI ETI, blurring zig-zag model immunity. In this review, we not only summarize our current understanding PRR- NLR-mediated immunities plants, but also highlight those components evolutionarily conserved across kingdom. Altogether, attempt offer overview how plants mediate integrate induction defense responses comprise emphasizing need for more evolutionary molecular plant-microbe interactions (EvoMPMI) will pave way a better emergence core machinery so-called arms race microbes.

Language: Английский

Citations

20
Nuclear transport receptor KA120 regulates molecular condensation of MAC3 to coordinate plant immune activation DOI Creative Commons
Min Jia, Xuanyi Chen, Xuetao Shi

et al.

Cell Host & Microbe, Journal Year: 2023, Volume and Issue: 31(10), P. 1685 - 1699.e7

Published: Sept. 15, 2023

Language: Английский

Citations

12
Lack of AtMC1 catalytic activity triggers autoimmunity dependent on NLR stability DOI Creative Commons
Jose Salguero‐Linares, Laia Armengot, Joel Ayet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

ABSTRACT Plants utilize cell surface-localized pattern recognition receptors (PRRs) and intracellular nucleotide-binding leucine-rich repeat (NLR) to detect non-self elicit robust immune responses. Fine-tuning the homeostasis of these is critical prevent their hyperactivation. Here, we show that Arabidopsis plants lacking metacaspase 1 ( At MC1) display autoimmunity dependent on signalling components downstream NLR PRR activation. Overexpression a catalytically inactive MC1 in an atmc1 background triggers severe partially same components. E3 ligase SNIPER1, master regulator homeostasis, fully reverts AtMC1-dependent phenotype, inferring broad defect turnover may underlie phenotype observed. Catalytically localizes punctate structures are degraded through autophagy. Altogether considering previous evidence proteostatic functions MC1, speculate Wt either directly or indirectly control protein levels, thereby preventing autoimmunity.

Language: Английский

Citations

0