Comprehensive identification of GASA genes in sunflower and expression profiling in response to drought DOI Creative Commons
Muhammad Asad Ullah,

Muhammad Ahmed,

Latifa AlHusnain

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 14, 2024

Drought stress poses a critical threat to global crop yields and sustainable agriculture. The GASA genes are recognized for their pivotal role in tolerance plant growth, but little is known about how they function sunflowers. investigation aimed identify elucidate the of HaGASA conferring sunflowers with drought tolerance. Twenty-seven different gene family members were found this study that inconsistently located across eleven sunflower chromosomes. Phylogeny analysis revealed divided into five subgroups by comparing those from Arabidopsis, peanut, soybean, within each subgroup displaying similar conserved motifs structures. In-silico evaluation cis-regulatory elements indicated existence specific associated stress-responsiveness being most abundant, followed hormone, light, growth-responsive elements. Transcriptomic data NCBI database was utilized assess expression profile varieties under conditions. ten genotypes stress, 14 differentially expressed genes, implying active plant's response. organs HaGASA2, HaGASA11, HaGASA17, HaGASA19, HaGASA21 HaGASA26 displayed maximum stem. Our findings implicate mediating growth maintenance adaptation abiotic particularly drought. findings, taken together, provided basic understanding structure potential functions setting framework further functional investigations roles mitigation improvement strategies.

Language: Английский

A reference-grade genome of the xerophyte Ammopiptanthus mongolicus sheds light on its evolution history in legumes and drought-tolerance mechanisms DOI Creative Commons
Lei Feng, Fei Teng, Na Li

et al.

Plant Communications, Journal Year: 2024, Volume and Issue: 5(7), P. 100891 - 100891

Published: April 1, 2024

Plants that grow in extreme environments represent unique sources of stress-resistance genes and mechanisms. Ammopiptanthus mongolicus (Leguminosae) is a xerophytic evergreen broadleaf shrub native to semi-arid desert regions; however, its drought-tolerance mechanisms remain poorly understood. Here, we report the assembly reference-grade genome for A. mongolicus, describe evolutionary history within legume family, examine The assembled 843.07 Mb length, with 98.7% sequences successfully anchored nine chromosomes mongolicus. predicted contain 47 611 protein-coding genes, 70.71% composed repetitive sequences; these are dominated by transposable elements, particularly long-terminal-repeat retrotransposons. Evolutionary analyses revealed two whole-genome duplication (WGD) events at 130 58 million years ago (mya) shared genus other legumes, but no species-specific WGDs were found this genus. Ancestral reconstruction has undergone fewer rearrangements than genomes confirming status as "relict plant". Transcriptomic demonstrated involved cuticular wax biosynthesis transport highly expressed, both under normal conditions response polyethylene glycol-induced dehydration. Significant induction related ethylene signaling was also observed leaves dehydration stress, suggesting enhanced formation thick waxy cuticles major drought tolerance Ectopic expression AmERF2, an factor can markedly increase transgenic Arabidopsis thaliana plants, demonstrating potential application crop improvement.

Language: Английский

Citations

10
Haplotype-resolved T2T genome assemblies and pangenome graph of pear reveal diverse patterns of allele-specific expression and the genomic basis of fruit quality traits DOI Creative Commons

Qionghou Li,

Xin Qiao, Lanqing Li

et al.

Plant Communications, Journal Year: 2024, Volume and Issue: 5(10), P. 101000 - 101000

Published: June 10, 2024

Hybrid crops often exhibit increased yield and greater resilience, yet the genomic mechanism(s) underlying hybrid vigor or heterosis remain unclear, hindering our ability to predict expression of phenotypic traits in breeding. Here, we generated haplotype-resolved T2T genome assemblies two pear varieties, 'Yuluxiang' (YLX) 'Hongxiangsu' (HXS), which share same maternal parent but differ their paternal parents. We then used these explore genome-scale landscape allele-specific (ASE) create a pangenome graph for pear. ASE was observed close 6000 genes both cultivars. A subset related aspects fruit quality such as sugars, organic acids, cuticular wax were identified, suggesting important contributions heterosis. Specifically, Ma1, gene regulating acidity, is absent haplotypes HXS YLX. built based on seven published genomes. Resequencing data 139 cultivated genotypes (including 97 sequenced here) subsequently aligned graph, revealing numerous structural variant hotspots selective sweeps during diversification. As predicted, Ma1 allele found be varieties with low acid content, this association functionally validated by overexpression calli. Overall, results reveal fruit-quality provide robust reference high-resolution discovery mapping.

Language: Английский

Citations

8
Lignin synthesis plays an essential role in the adaptation of Haloxylon ammodendron to adverse environments DOI
Xin-Pei Lü,

Zhao‐Long Lü,

Yuming Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142321 - 142321

Published: March 1, 2025

Language: Английский

Citations

0
The salt bladder is essential for Atriplex canescens in response to salinity by regulating the ion homeostasis and water balance DOI
Shan Wang, Le Zhang, Huan Guo

et al.

Plant and Soil, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Language: Английский

Citations

0
HKT1;1 modulated by vacuolar Na+ compartmentation functions in maintaining the salt-accumulating trait in a xerophyte DOI

Wenying Wang,

Zhijie Ren,

Peiqin Li

et al.

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 226, P. 105921 - 105921

Published: July 29, 2024

Language: Английский

Citations

1
Comprehensive identification of GASA genes in sunflower and expression profiling in response to drought DOI Creative Commons
Muhammad Asad Ullah,

Muhammad Ahmed,

Latifa AlHusnain

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 14, 2024

Drought stress poses a critical threat to global crop yields and sustainable agriculture. The GASA genes are recognized for their pivotal role in tolerance plant growth, but little is known about how they function sunflowers. investigation aimed identify elucidate the of HaGASA conferring sunflowers with drought tolerance. Twenty-seven different gene family members were found this study that inconsistently located across eleven sunflower chromosomes. Phylogeny analysis revealed divided into five subgroups by comparing those from Arabidopsis, peanut, soybean, within each subgroup displaying similar conserved motifs structures. In-silico evaluation cis-regulatory elements indicated existence specific associated stress-responsiveness being most abundant, followed hormone, light, growth-responsive elements. Transcriptomic data NCBI database was utilized assess expression profile varieties under conditions. ten genotypes stress, 14 differentially expressed genes, implying active plant's response. organs HaGASA2, HaGASA11, HaGASA17, HaGASA19, HaGASA21 HaGASA26 displayed maximum stem. Our findings implicate mediating growth maintenance adaptation abiotic particularly drought. findings, taken together, provided basic understanding structure potential functions setting framework further functional investigations roles mitigation improvement strategies.

Language: Английский

Citations

1